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Protein Concentration SOP: Bradford Assay

Shalaka Samant, PhD • January 21, 2020

SOP: Protein Reagent Concentration

Determining Sample Consistency

Whether your lab already has an SOP in place for new protein analysis or would benefit from a revised procedure, we provide a simplified version of kbDNAs in-house Bradford Assay protocol for concentration testing commercial protein reagents.

Why concentration and why now?

> LAB REPLENISHING

Many researchers have begun ordering recombinant reagents as part of their material replenishing & it's important to revisit the role of concentration

> COVID IMPACT

Due to the unique disruption in the manufacturing sector, the likelihood of receiving proteins from the same batch (or lot) prior to COVID is low

> CONCENTRATION FACTOR

Measuring sample concentration and comparing it to the data on the certificate of analysis is the most direct way to determine whether any quality was impacted

> VERIFYING QUALITY

We suggest running Bradford assay tests on both same and different lot samples since the disruption could have occurred during the production or storage period

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