Welcome to the kbDNA Publishing Lab

Information plays a structural role in scientific research. At kbDNA, we perceive information as the most valuable material any organization can offer. That is why we dedicate our efforts to continuously providing novel, relevant data and insight to all researchers. Our pieces present organizational theories via opinion-editorials and trend analysis white papers. Technical notes, protocols and figures are all generated straight from the lab team to support design of experiments and application technique. The kbDNA Publishing Lab is designed to bridge research labs with the often-unfamiliar knowledge of the manufacturing lab that could be a valuable resource.

The latest from kbDNA
Utilizing Nucleotide-Based Methods for Optimal Detection
Technical Note
09/22/2020 3:09 am
The application of oligonucleotide-aptamer assays have been proven tactical in accelerating drug discovery platforms. Whether it is through single-site or dual-site binding functionality, learn the advantages of aptamers in detecting your target molecules
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Addressing the Poor Stability Issues in Fusion Protein Cleavage
Technical Note
09/08/2020 6:09 pm
By analyzing the performance of 3 major proteases, this review covers the issues surrounding instability and fusion protein cleavage along with suggesting technical countermeasures. Analysis screened the enzymatic stability, efficiency in removing the GST-fusion partner and proteolytic specificity cleaving a diverse range of fusion proteins.
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Cleavage of GST Fusion Proteins With PreScission Protease
Protocol
09/08/2020 6:09 pm
The following protocol outlines specified steps for optimized cleavage of GST-fusion proteins tagged to a protein of interest using PreScission Protease
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Viral RNA Isolation Methods Reviewed: Spin vs. Magnetic
Technical Note
05/22/2020 1:05 pm
COVID19 research has prompted an upsurge in routine RNA methods for analyzing a "nonroutine" (novel) disease. Whether it's for sequencing or PCR methods; preparation of the target RNA is critical for driving the downstream application results. In this technical note, we revisit nucleic acid extraction prep and review mainstream methods in the context of isolating VIRAL RNA from its sample. Consider our insight in your lab and see if it can help optimize your sample prep or maybe save on material costs!
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Can the Application of siRNA Help Improve Our Targeting of Coronavirus?
Op Ed
04/07/2020 9:04 pm
As the focus on COVID19 continues to accelerate, we explore the topic of drug targeting selectivity with respect to coronaviruses. By reviewing key genomic and structural elements of the virus; this piece provides an understanding about the research challenges and makes a case for siRNA tools as a means of target optimization.
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Application of Covalent Bonds Between Small Molecules and Proteins for Improved Drug Discovery
Op Ed
02/23/2020 8:02 pm
The application of covalent binders in mainstream drugs sheds light on the value of the reactivity relationships for activating specific covalent bonds between nucleophilic proteins & electrophilic small molecules. This understanding has led drug hunters to further investigate the potential of employing covalent molecules earlier in the process to support their drug discovery processes. This piece aims to survey key information needed to start understanding these concepts & support your drug discovery.
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Characterization of Multi-Protein Complexes (MPCs) by BN-PAGE
Protocol
12/17/2019 11:12 am
Multi-protein complexes (MPCs) play a crucial role in signaling. Blue native polyacrylamide gel electrophoresis (BN-PAGE) can be used for one-step isolation of MPCs from biological membranes/organelles and total cell and tissue homogenates under native conditions.
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The Synthetic Dogma
Op Ed
12/03/2019 11:12 pm
How natures molecules flow in bioresearch
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Microarray Spotting & Hybridization
Protocol
11/19/2019 7:11 pm
In this piece, we present a series of protocols that suggest optimal preparation standards during the process of spotting and arraying microarrays relative to epoxy, aldehyde, and amine coated slides.
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Compounds for DNA Synthesis
Technical Note
11/17/2019 2:11 pm
See the full list of in-house DNA Compounds used in our synthesis
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DNA Modifiers
Technical Note
11/17/2019 2:11 pm
See the full list of in-house DNA Modifiers used in our synthesis
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Compounds for RNA Synthesis
Technical Note
11/17/2019 2:11 pm
See the full list of in-house RNA Compounds used in our synthesis
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RNA Modifiers
Technical Note
11/17/2019 2:11 pm
See the full list of in-house RNA Modifiers used in our synthesis
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Reagents for Nucleic Acid Chemistry
Technical Note
11/17/2019 2:11 pm
See the full list of in-house Reagents for Nucleic Acid Chemistry used in our synthesis
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Nucleosides & Amidites
Technical Note
11/17/2019 2:11 pm
See the full list of in-house Amidites used in our synthesis
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Molecular-Weight Standards Chart : Proteins
Technical Note
06/17/2019 2:06 am
REFERENCE PROTEINS AS MOLECULAR-WEIGHT STANDARDS
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How to Improve Your Search for Reagents
Op Ed
06/04/2019 6:06 pm
Key takeaways from our experience building and advancing a reagent-specific search tool followed by our decision to drop the entire approach. In this editorial we provide helpful insight about the limitations of search functionality and the relevant information to consider when sourcing your next recombinant protein.
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Top Ten Modifications for Nucleotide Synthesis
Technical Note
05/08/2019 8:05 pm
To further bridge the gap between designing a DNA or RNA sequence and the process of synthesizing it - this review offers a fundamental break-down of the core modifications that help support novel synthesis.
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Obsessing Over CRISPR Isn’t Making It Any Safer, but RNA Might
Op Ed
05/01/2019 5:05 pm
As CRISPR continues to innovate our lab methods and dominate our news feeds; its issues with off-targeting continue to limit downstream potential. Progress may be moving at a less favorable pace, but recent methods in novel modifications to the guide RNA (gRNA) structure may provide the necessary traction to move toward the right solutions.
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An Updated Overview of Protein Tags
Technical Note
04/23/2019 1:04 pm
A helpful guide for the variety of different protein tags - trending in recent growth
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It May Seem like It’s Raining Reagents, but It’s Only a Flood of Companies with the Same Catalogs
Op Ed
04/16/2019 2:04 pm
Understanding the back-end phenomenon of commercial reagents and the factors limiting their success in ecommerce.
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Difficult-To-Express Proteins Are Not Going to Solve Themselves
Op Ed
04/09/2019 10:04 pm
It always seems like it’s raining recombinant proteins, until an experiment requires a difficult target. Then it just seems like an endless loop of identical catalogs from different companies. This has been a growing source of frustration for both manufacturers and researchers. Read our break-down about the difficult-to-express situation and what technology we believe bring hope to solutions...
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Rethink Modifications
Technical Note
03/20/2019 12:03 am
A guide to modifying sequences for synthesis
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Oligos: How the Lego Pieces of DNA Are Shaping Research
Op Ed
03/06/2019 2:03 pm
Discussing current progress in oligonucleotides and why synthesis technology will play a key role toward future innovation
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Synthesis Attachment Chemistry
Technical Note
02/20/2019 3:02 pm
Review of the technical components that drive attachment chemistry in nucleotide synthesis
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2018 Featured Blogs
Op Ed
06/30/2018 11:06 am
Developing a bio-website and the reqarding irony in failure throughout the process
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