Protocols, Technical Notes, & Op-Ed

Welcome to the kbDNA Publishing Lab

Information plays a structural role in scientific research. At kbDNA, we perceive information as the most valuable material any organization can offer. That is why we dedicate our efforts to continuously providing novel, relevant data and insight to all researchers. Our pieces present organizational theories via opinion-editorials and trend analysis white papers. Technical notes, protocols and figures are all generated straight from the lab team to support design of experiments and application technique. The kbDNA Publishing Lab is designed to bridge research labs with the often-unfamiliar knowledge of the manufacturing lab that could be a valuable resource.

Utilizing Nucleotide-Based Methods for Optimal Detection

Oligonucleotide aptamer assays have been proven tactical in accelerating drug discovery platforms. Single-site or dual-site binding, the utility of aptamer science offers significant advantages in detecting your target molecules. Learn about oligo-aptamer assays & consider their application in your experiment workflow.

Addressing the Poor Stability Issues in Fusion Protein Cleavage

Protein instability analysis screening enzymatic stability activity, efficiency removing GST fusion partner and proteolytic specificity cleaving a diverse range of fusion protein tags using PreScission, factor Xa, and thrombin protease.

Cleavage of GST Fusion Proteins With PreScission Protease

Highly specific and optimized protocol for cleavage of GST fusion protein tag to a protein of interest or target reagent using PreScission Protease on column or cleavage in solution method.

Viral RNA Isolation Methods Reviewed: Spin vs. Magnetic

The upsurge in infectious disease research prompts immense RNA isolation techniques for sample preparation. High purity extraction of the viral RNA is critical for characterization analysis and drug discovery. Learn how spin-column and magnetic bead-based method compare when it comes to your viral RNA extraction.

Can the Application of siRNA Help Improve Our Targeting of Coronavirus?

Explore the topic of drug targeting selectivity in coronaviruses. Analysis of key genomic & structural elements of the virus provides an understanding of the research challenges while making a case for siRNA synthetic reagents for antisense target optimization.

Application of Covalent Bonds Between Small Molecules and Proteins for Improved Drug Discovery

Recent success in targeting covalent inhibitors has increased consideration of covalent molecules in target discovery. Gain insight on the current nucleophile modalities, electrophile warheads & innovative methods helping drug hunters advance small-molecule research.

Characterization of Multi-Protein Complexes (MPCs) by BN-PAGE

Multi-protein complexes (MPCs) play a crucial role in signaling. Blue native polyacrylamide gel electrophoresis (BN-PAGE) can be used for one-step isolation of MPCs from biological membranes/organelles and total cell and tissue homogenates under native conditions.

The Synthetic Dogma

Relative to the central dogma of molecular biology, our synthetic dogma explains the profiles of DNA, RNA & Protein as analyte-specific research reagents and their applications throughout the workflow of experiments for drug discovery; ssDNA, dsRNA, recombinants

Microarray Spotting & Hybridization

In this piece, we present a series of protocols that suggest optimal preparation standards during the process of spotting and arraying microarrays relative to epoxy, aldehyde, and amine coated slides.

Compounds for DNA Synthesis

See the full list of in-house DNA Compounds used in our synthesis

See the full list of in-house DNA Modifiers used in our synthesis

Compounds for RNA Synthesis

See the full list of in-house RNA Compounds used in our synthesis

See the full list of in-house RNA Modifiers used in our synthesis

Reagents for Nucleic Acid Chemistry

See the full list of in-house Reagents for Nucleic Acid Chemistry used in our synthesis

Nucleosides & Amidites

See the full list of in-house Amidites used in our synthesis

Molecular-Weight Standards Chart : Proteins

REFERENCE PROTEINS AS MOLECULAR-WEIGHT STANDARDS

How to Improve Your Search for Reagents

Sick of chasing material data sheets & using reagent search tools that are either too specific or not specific enough? kbDNA matches laboratories with the right reagents for their research. Learn about the limitations of search functionality & the relevant information to consider when sourcing recombinant protein.

Top Ten Modifications for Nucleotide Synthesis

To further bridge the gap between designing a DNA or RNA sequence and the process of synthesizing it - this review offers a fundamental break-down of the core modifications that help support novel synthesis.

Obsessing Over CRISPR Isn’t Making It Any Safer, but RNA Might

CRISPR/CAS9 is innovating drug discovery & laboratory research methods, but off-targeting remains an issue. Modifying the single guide RNA emerges as an effective solution. Rational design & engineering secondary structure offer capabilities to apply diverse gRNA types as hairpins to optimize specificity.

An Updated Overview of Protein Tags

A helpful guide for the variety of different protein tags - trending in recent growth

It May Seem like It’s Raining Reagents, but It’s Only Companies with the Same Catalogs

Commercializing synthetic research reagents is very complex in the life science industry. Companies are attempting to address the challenges of sourcing proper reagents by way of ecommerce innovation and are faced with the same bottleneck. Discover what factors are limiting product diversity in research.

Difficult-To-Express Proteins Are Not Going to Solve Themselves

Reagents seem abundant in the marketplace, until you need to source difficult-to-express recombinant protein, thus exposing the limited manufacturing capacity of many vendors. Demand for research is growing beyond basic protein catalogs. Learn how kbDNA's versatile synthesis offers multi-dimer & low molecular weight proteins.

Rethink Modifications

A guide to modifying sequences for synthesis

Oligos: How the Lego Pieces of DNA Are Shaping Research

ssDNA, siRNA, gRNA, LNA, BNA & more Novel breakthroughs in oligonucleotides for life science research and drug discovery. Discover how versatile synthesis is innovating automated synthesis and shaping the future of biotechnology.

Synthesis Attachment Chemistry

Review of the technical components that drive attachment chemistry in nucleotide synthesis

Bootstrapping a Biotech

Developing a bio-website and the ironic value in small failures throughout the process.