Welcome to the kbDNA Publishing Lab

Information plays a structural role in scientific research. At kbDNA, we perceive information as the most valuable material any organization can offer. That is why we dedicate our efforts to continuously providing novel, relevant data and insight to all researchers. Our pieces present organizational theories via opinion-editorials and trend analysis white papers. Technical notes, protocols and figures are all generated straight from the lab team to support design of experiments and application technique. The kbDNA Publishing Lab is designed to bridge research labs with the often-unfamiliar knowledge of the manufacturing lab that could be a valuable resource.

Technical Note

The Rise of Cryo-EM Among Structural Characterization Methods: NMR, X-Ray Crystallography

Characterization plays a comprehensive role throughout discovery. For structural biologists, this entails gaining a better understanding of biophysical and dynamic properties relative to their research molecules. While various disciplines continue to help innovate methods used for structural studies-the recent introduction of cryo-electron microscopy (cryo-EM) has been a significant addition to the experimental workflow. In this note, we review and compare primary methods for structural characterization, while exploring the true application potential for Cryo-EM.
12/01/2020

White Paper

Optimizing CRO Strategies For Discovery Research Projects

Current trends in outsourcing are relatively uncommon in early stage research and present exclusive challenges for discovery teams. Our paper explores the role of data uncertainty, concept validation and technical consistency in defining discovery stage objectives. We outline four major approaches for better data management and communication to optimize a laboratory’s CRO strategy
11/23/2020

White Paper

Biochemical Overview of Key Post-Translational Modifications

Many of the research complications that bridge gaps between molecule and mechanism involve the post-translational modifications (PTMs) of the proteins of interest. PTMs also play a unique role in manufacturing these proteins as reagents. In order to understand their process, we've generated a comprehensive white paper - outlining the up-to-date biochemistry of key PTMs disrupting research to date.
11/23/2020

Op Ed

Aptamers & Antibodies: A Cooperation, Not a Competition

Reviewing the conventional aptamer : antibody side by side comparison. By utilizing our professional experiences with both reagents to highlight some essential oversights between the two, we make a case for implementing both reagents in your experimental workflow to reinforce your discovery platform. Read more about it in this op-ed article!
10/21/2020

Technical Note

Top Ten Modifications for Nucleotide Synthesis

To further bridge the gap between designing a DNA or RNA sequence and the process of synthesizing it - this review offers a fundamental break-down of the core modifications that help support novel synthesis.
10/14/2020

Technical Note

Utilizing Nucleotide-Based Methods for Optimal Detection

Oligonucleotide aptamer assays have been proven tactical in accelerating drug discovery platforms. Single-site or dual-site binding, the utility of aptamer science offers significant advantages in detecting your target molecules. Learn about oligo-aptamer assays & consider their application in your experiment workflow.
09/22/2020

Technical Note

Addressing the Poor Stability Issues in Fusion Protein Cleavage

Protein instability analysis screening enzymatic stability activity, efficiency removing GST fusion partner and proteolytic specificity cleaving a diverse range of fusion protein tags using PreScission, factor Xa, and thrombin protease.
09/08/2020

Protocol

Cleavage of GST Fusion Proteins With PreScission Protease

Highly specific and optimized protocol for cleavage of GST fusion protein tag to a protein of interest or target reagent using PreScission Protease on column or cleavage in solution method.
09/08/2020

Technical Note

Viral RNA Isolation Methods Reviewed: Spin vs. Magnetic

The upsurge in infectious disease research prompts immense RNA isolation techniques for sample preparation. High purity extraction of the viral RNA is critical for characterization analysis and drug discovery. Learn how spin-column and magnetic bead-based method compare when it comes to your viral RNA extraction.
05/22/2020

Op Ed

Can the Application of siRNA Help Improve Our Targeting of Coronavirus?

Explore the topic of drug targeting selectivity in coronaviruses. Analysis of key genomic & structural elements of the virus provides an understanding of the research challenges while making a case for siRNA synthetic reagents for antisense target optimization.
04/07/2020

Op Ed

Application of Covalent Bonds Between Small Molecules and Proteins for Improved Drug Discovery

Recent success in targeting covalent inhibitors has increased consideration of covalent molecules in target discovery. Gain insight on the current nucleophile modalities, electrophile warheads & innovative methods helping drug hunters advance small-molecule research.
02/23/2020

Protocol

Characterization of Multi-Protein Complexes (MPCs) by BN-PAGE

Multi-protein complexes (MPCs) play a crucial role in signaling. Blue native polyacrylamide gel electrophoresis (BN-PAGE) can be used for one-step isolation of MPCs from biological membranes/organelles and total cell and tissue homogenates under native conditions.
12/17/2019

Op Ed

The Synthetic Dogma

Relative to the central dogma of molecular biology, our synthetic dogma explains the profiles of DNA, RNA & Protein as analyte-specific research reagents and their applications throughout the workflow of experiments for drug discovery; ssDNA, dsRNA, recombinants
12/03/2019

Protocol

Microarray Spotting & Hybridization

In this piece, we present a series of protocols that suggest optimal preparation standards during the process of spotting and arraying microarrays relative to epoxy, aldehyde, and amine coated slides.
11/19/2019

Technical Note

DNA Modifiers

See the full list of in-house DNA Modifiers used in our synthesis
11/17/2019

Op Ed

How to Improve Your Search for Reagents

Sick of chasing material data sheets & using reagent search tools that are either too specific or not specific enough? kbDNA matches laboratories with the right reagents for their research. Learn about the limitations of search functionality & the relevant information to consider when sourcing recombinant protein.
06/04/2019

Op Ed

Obsessing Over CRISPR Isn’t Making It Any Safer, but RNA Might

CRISPR/CAS9 is innovating drug discovery & laboratory research methods, but off-targeting remains an issue. Modifying the single guide RNA emerges as an effective solution. Rational design & engineering secondary structure offer capabilities to apply diverse gRNA types as hairpins to optimize specificity.
05/01/2019

Technical Note

An Updated Overview of Protein Tags

A helpful guide for the variety of different protein tags - trending in recent growth
04/23/2019

Op Ed

It May Seem like It’s Raining Reagents, but It’s Only Companies with the Same Catalogs

Commercializing synthetic research reagents is very complex in the life science industry. Companies are attempting to address the challenges of sourcing proper reagents by way of ecommerce innovation and are faced with the same bottleneck. Discover what factors are limiting product diversity in research.
04/16/2019

Op Ed

Difficult-To-Express Proteins Are Not Going to Solve Themselves

Reagents seem abundant in the marketplace, until you need to source difficult-to-express recombinant protein, thus exposing the limited manufacturing capacity of many vendors. Demand for research is growing beyond basic protein catalogs. Learn how kbDNA's versatile synthesis offers multi-dimer & low molecular weight proteins.
04/09/2019

Technical Note

Rethink Modifications

A guide to modifying sequences for synthesis
03/20/2019

Op Ed

Oligos: How the Lego Pieces of DNA Are Shaping Research

ssDNA, siRNA, gRNA, LNA, BNA & more Novel breakthroughs in oligonucleotides for life science research and drug discovery. Discover how versatile synthesis is innovating automated synthesis and shaping the future of biotechnology.
03/06/2019

Op Ed

Bootstrapping a Biotech

Developing a bio-website and the ironic value in small failures throughout the process.
06/30/2018