Compounds for RNA Synthesis
L L • November 30, 2017
RNA Synthesis
See the full list of in-house RNA Compoundsused in our synthesis
Improving RNA Synthesis
The synthesis of RNA is conventionally more sensitive than DNA. This poses challenges in developing very similar synthesis compounds such as amidites and supports, but with the right chemistry to counter RNA-specific hurdles. Stability and structural confirmation are routine offenders, and both can be countered by utilizing backbone phosphorothioation and reverse synthesis techniques. β-L-RNA-Spiegelmers and minor RNA phosphoramidites offer novel solutions to end-user specificity. While TOM-amidites are highly suitable for large scale therapeutic grade RNA synthesis, long RNA sequences, aptamers, and biologically significant RNA molecules.
See the full list of in-house RNA Compounds used in our synthesis below:
RNA Synthesis
A: CE Phophoroamidites: TBDMS Protected
| (1)-TBDMS Protected |
|---|
| Adenosine ( N -Ac)-DMTr-3'-CEP |
| Adenosine ( N -Bz)-DMTr-3'-CEP |
| Adenosine (N-Bz)-MMTr-3'-CEP |
| Cytidine ( N -Ac)-DMTr-3'-CEP |
| Cytidine ( N -Bz)-DMTr-3'-CEP |
| Cytidine ( N -Bz)-MMTr-3'-CEP |
| Guanosine ( N -Ac)-DMTr-3'-CEP |
| Guanosine ( N -DMF)-DMTr-3'-CEP |
| Guanosine ( N -iBu)-DMTr-3'-CEP |
| Guanosine ( N -iBu)-MMTr-3'-CEP |
| Uridine-DMTr-3'-CEP |
| Uridine-MMTr-3'-CEP |
| (2)-TBDMS Protected (Ultramild base protection) |
|---|
| Adenosine ( N -PAC)-DMTr-3'-CEP |
| Adenosine ( N -PAC)-MMTr-3'-CEP |
| Cytidine ( N -Ac)-DMTr-3'-CEP |
| Cytidine ( N -PAC)-DMTr-3'-CEP |
| Cytidine ( N -tBPAC)-MMTr-3'-CEP |
| Guanosine ( N -iprPAC)-DMTr-3'-CEP |
| Guanosine ( N -PAC)-DMTr-3'-CEP |
| Guanosine ( N -PAC)-MMTr-3'-CEP |
| Guanosine ( N -Phenyl-Ac)-DMTr-3'-CEP |
| (3)-TOM Protected |
|---|
| 2'-TOM-Adenosine ( N -Ac)-DMTr-3'-CEP |
| 2'-TOM-Cytidine ( N -Ac)-DMTr-3'-CEP |
| 2'-TOM-Guanosine ( N -Ac)-DMTr-3'-CEP |
| 2'-TOM-Uridine-DMTr-3'-CEP |
| (4)-Acetal levulinyl ester Protected |
|---|
| 2'-ALE-Adenosine ( N -PAc)-5'-NPPOC-3'-CEP |
| 2'-ALE-Cytidine ( N -iBu)-5'-NPPOC-3'-CEP |
| 2'-ALE-Guanosine ( N -PAc)-5'-NPPOC-3'-CEP |
| 2'-ALE-Uridine-5'-NPPOC-3'-CEP |
| (5)-Pivaloyloxymethyl Protected |
|---|
| 2'-Pivom-Adenosine ( N -PAc)-DMTr-3'-CEP |
| 2'-Pivom-Cytidine ( N -Ac)-DMTr-3'-CEP |
| 2'-Pivom-Guanosine ( N -iPrPAC)-DMTr-3'-CEP |
| 2'-Pivom-Uridine-DMTr-3'-CEP |
B: Supports
| (1)-TBDMS Protected 3'-CPG |
|---|
| Adenosine ( N -Bz)-DMTr-3'-Icaa CPG |
| Cytidine ( N -Ac)-DMTr-3'-Icaa CPG |
| Cytidine ( N -Bz)-DMTr-3'-Icaa CPG |
| Guanosine ( N -iBu)-DMTr-3'-Icaa CPG |
| Uridine-DMTr-3'-Icaa CPG |
| (2)-TBDMS protected 3'-CPG (Ultramild base protection) |
|---|
| Adenosine ( N -PAC)-DMTr-3'-Icaa CPG |
| Adenosine ( N -tBPAC)-DMTr- 3'-Icaa CPG |
| Cytidine ( N -PAC)-DMTr-3'-Icaa CPG |
| Guanosine ( N -PAC)-DMTr-3'-Icaa CPG |
| Guanosine ( N -tBPAC)-DMTr-3'-Icaa CPG |
| Uridine-DMTr-3'-Icaa CPG |
| (3)-TBDMS protected 3'-Oxalyl CPG |
|---|
| Adenosine ( N -PAC)-DMTr-3'-oxalyl Icaa-CPG |
| Ammonia Free Oligo Deprotection Solution |
| Cytidine ( N -PAC)-DMTr-3'-oxalyl Icaa CPG |
| Guanosine ( N -iBu)-DMTr-3'-oxalyl Icaa CPG |
| Guanosine ( N -PAC)-DMTr-3'-oxalyl Icaa CPG |
| Uridine-DMTr-3'-oxalyl Icaa CPG |
| (4)-TBDMS protected 3'-Polystyrene |
|---|
| Adenosine ( N -Bz)-DMTr-3'-succinyl Polystyrene |
| Cytidine ( N -Ac)-DMTr-3'-succinyl Polystyrene |
| Cytidine ( N -Bz)-DMTr-3'-succinyl Polystyrene |
| Guanosine ( N -iBu)-DMTr-3'-succinyl Polystyrene |
| Uridine-DMTr-3'-succinyl Polystyrene |
| (5)-TBDMS protected-3'-Non-clevable long chain spacer |
|---|
| 2'-TBDMS Guanosine ( N -iBu)-DMTr-3'-non-clevable long chain space |
| (6)-TOM protected 3'-CPG |
|---|
| 2'-TOM-Adenosine ( N -Ac)-DMTr-3'-Icaa CPG |
| 2'-TOM-Cytidine ( N -Ac)-DMTr-3'-Icaa CPG |
| 2'-TOM-Guanosine ( N -Ac)-DMTr-3'-Icaa CPG |
| 2'-TOM-Uridine-DMTr-3'-Icaa CPG |
C: Backbone modification:Methyl Phosphoramidite
| P-O -Methyl-adenosine ( N -Bz)-DMTr-3'-CEP |
|---|
| P-O -Methyl-adenosine ( N -Bz)-MMTr-3'-CEP |
| P-O -Methyl-adenosine ( N -PAC)-DMTr-3'-CEP |
| P-O -Methyl-adenosine ( N -PAC)-MMTr-3'-CEP |
| P-O -Methyl-cytidine ( N -Bz)-DMTr-3'-CEP |
| P-O -Methyl-cytidine ( N -PAC)-DMTr-3'-CEP |
| P-O -Methyl-guanosine ( N -iBu)-DMTr-3'-CEP |
| P-O -Methyl-guanosine ( N -iBu)-MMTr-3'-CEP |
| P-O -Methyl-guanosine ( N -PAC)-DMTr-3'-CEP |
| P-O-Methyl-guanosine ( N -PAC)-MMTr-3'-CEP |
| P-O -Methyl-uridine-DMTr-3'-CEP |
RNA: 5'→ 3' Synthesis
A: CE Phophoroamidites: TBDMS Protected
| A: CE Phosphoramidites: TBDMS Protected |
|---|
| Adenosine ( N -Bz)-DMTr-5'-CEP |
| Cytidine ( N -Ac)-DMTr-5'-CEP |
| Cytidine ( N -Bz)-DMTr-5'-CEP |
| Guanosine ( N -iBu)-DMTr-5'-CEP |
| Guanosine ( N -iprPAC)-DMTr-5'-CEP |
| Uridine-DMTr-5'-CEP |
| B: Supports-TBDMS protected 5'-CPG |
|---|
| Adenosine ( N -Bz)-DMTr-5'-Icaa CPG |
| Cytidine ( N -Ac)-DMTr-5'-Icaa CPG |
| Cytidine ( N -Bz)-DMTr-5'-Icaa CPG |
| Guanosine ( N -iBu)-DMTr-5'-Icaa CPG |
| Uridine-DMTr-5'-Icaa CPG |
Minor RNA Bases
A: Minor RNA Phosphoramidites
| (1)-TBDMS Protected RNA Phosphoramidites |
|---|
| 2,2'-Anhydro-uridine-DMTr-3'-CEP |
| 2-Amino-adenosine-DMTr-3'-CEP, (2,6-Diamino Purine DMTr-3'-CEP) |
| 2-Amino-purine ( N -DMF)-DMTr-3'-CEP |
| 2-Amino-purine ( N -iBu)-DMTr-3'-CEP |
| 2-One-pyridine-DMTr-3'-CEP; Zebularine CEP |
| 4-Triazolyl-uridine-DMTr-3'-CEP |
| 4-Thio-uridine-DMTr-3'-CEP |
| 2-Thio-uridine-DMTr-3'-CEP |
| 5-Bromo-cytidine ( N -Bz)-DMTr-3'-CEP |
| 5-Bromo-uridine-DMTr-3'-CEP |
| 5-Fluoro-2'-deoxyuridine-DMTr-3'-CEP |
| 5-Iodo-cytidine ( N -Bz)-DMTr-3'-CEP |
| 5-Iodo-uridine-DMTr-3'-CEP |
| 5-Methyl-cytidine ( N -Bz)-DMTr-3'-CEP |
| 8-Bromo-adenosine ( N -DBF)-DMTr-3'-CEP |
| 8-Bromo-adenosine ( N -DMF)-DMTr-3'-CEP |
| 8-Bromo-guanosine ( N -DMF)-DMTr-3'-CEP |
| 8-methyl-guanosine ( N -iBu)-DMTr-3'-CEP |
| 8-Oxo-7-allyl-guanosine ( N -iBu)-DMTr-3'-CEP |
| 8-Oxo-guanosine ( N -DMF)-DMTr-3'-CEP |
| Inosine-DMTr-3'-CEP |
| N 3 -Methyl-thymidine-DMTr-3'-CEP |
| N 3 -Methyl-uridine-DMTr-3'-CEP |
| N 3 -Thiobenzoylethyl-uridine-DMTr-3'-CEP |
| N 6 , N 6 -Dimethyl-adenosine-5'-DMTr-3'-CEP |
| N 6 -Benzyl-adenosine-DMTr-3'-CEP |
| N 6 -isopentenyl-adenosine-DMTr-3'-CEP |
| N 4 -Ethyl-cytidine-DMTr-3'-CEP |
| Purine-riboside-DMTr-3'-CEP; Nebularine CEP |
| (2)Amino TFA Protected RNA Phosphoramidites |
|---|
| 2'-Amino(TFA)-cytidine ( N -Bz)-DMTr-3'-CEP |
| 2-Amino-2'-Amino (TFA)-uridine-DMTr-3'-CEP |
| 5'-Amino (TFA)-cytidine ( N -Ac)-3'-CEP |
| (3)-Acetyl Protected RNA Phosphoramidites |
| 2',3'-Diacetyl-guanosine ( N -iBu)-5'-CEP |
| 2',3'-Diacetyl-uridine-5'-CEP |
B: Supports-Minor-RNA-TBDMS Protected 3'-CPG
| (1)-TBDMS Protected RNA 3'-CPG |
|---|
| 2,2'-Anhydro-uridine-DMTr-3'-Icaa CPG |
| 2-Amino-adenosine-DMTr-3'-Icaa CPG |
| 2-Amino-purine-DMTr-3'-Icaa CPG |
| 2-One-pyridine-DMTr-3'-Icaa CPG; Zebularine CPG |
| 5-Bromo-cytidine ( N -Bz)-DMTr-3'-Icaa CPG |
| 5-Bromo-uridine-DMTr-3'-Icaa CPG |
| 5-Fluoro-uridine-DMTr-3'-Icaa CPG |
| 5-Iodo-cytidine ( N -Bz)-DMTr-3'-Icaa CPG |
| 5-Iodo-uridine-DMTr-3'-Icaa CPG |
| 5-Methyl-cytidine ( N -Bz)-DMTr-3'-Icaa CPG |
| 7-Deaza-adenosine ( N -Bz)-DMTr-3'-Icaa CPG; Tubercidin CPG |
| 7-Deaza-guanosine ( N -iBu)-DMTr-3'-Icaa CPG |
| 8-Bromo-adenosine ( N -DBF)-DMTr-3'-Icaa CPG |
| 8-Methyl-guanosine ( N -iBu)-DMTr-3'-Icaa CPG |
| Adenosine ( N -Ac)-DMTr-3'-Icaa CPG |
| Adenosine ( N -Bz)-DMTr-3'-Icaa CPG |
| Cytidine ( N -Ac)-DMTr-3'-Icaa CPG |
| Cytidine ( N -Bz)-DMTr-3'-Icaa CPG |
| Guanosine ( N -iBu)-DMTr-3'-Icaa CPG |
| Inosine-DMTr-3'-Icaa CPG |
| Iso-Cytidine ( N -DBF)-DMTr-3'-Icaa CPG |
| Iso-Guanosine ( N -DBF)-DMTr-3'-Icaa CPG |
| N^3 -Methyl-thymidine-DMTr-3'-Icaa CPG |
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