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DNA Modifiers
L L • December 1, 2021
DNA Modifiers
See the full list of in-house DNA Modifiers used in our synthesis below:
Modifying DNA During Synthesis
Modifying DNA during synthesis involves a bit of creativity along with untraditional chemistry throughout the synthesis workflow. Different classes of modifiers have grown over time, but the most tried and true begins with 3’ or 5’ amino modifiers and the use of spacers and “branching” modifications. This paved the way for introducing more elaborate modifiers into sequences, such as compounded functional groups and a wide array of labels.
RNA DNA Modifiers
| A: Chemical Phosphorylation and Supports |
|---|
| 5'- Phosphorylation; Bis-cyanoethyl- N , N -diisopropyl CEP |
| 3'-Phosphate-ON Icaa CPG |
| 5-Phosphorylation (generates DMTr color)-(5-Phosphate-ON Reagent) |
| B: Amino Modifiers and Supports |
|---|
| 3'-Amino (Fmoc)-modifier DMTr CEP |
| 3'-Amino (Fmoc)-modifier-C3 CPG |
| 3'-Amino (Fmoc)-modifier-C7 CPG |
| 3'-Amino (TFA)-modifier-spacer 6 Polystyrene |
| 3'-Amino (TFA)-modifier-spacer 7-Icaa CPG |
| 3'-Amino (trityl)-modifier-dT CPG |
| 3'-Amino-modifier-C6-phthalamido CPG |
| 5'-Amino (Fmoc)-modifier-C6 CEP |
| 5'-Amino (Fmoc)-modifier-C7 CEP |
| 5'-Amino (MMTr)-modifiers-C6 CEP |
| 5'-Amino (TFA)-modifier-C12 CEP |
| 5'-Amino (TFA)-modifiers-C4 CEP |
| 5'-Amino (TFA)-modifiers-C5 CEP |
| 5'-Amino (TFA)-modifiers-C6 CEP |
| 5'-Amino(MMT)-modifier-C12 CEP |
| Amino-modifier-13-dC CE; deoxy Cytidine N 4 Amino Linker CEP |
| Amino-modifier-13-dC CPG |
| Amino-modifier-C6-dT CPG |
| Amino-modifier-C6-phthalamido CEP |
| Amino-modifiers-C6-dT CEP |
| DMT-free Amine C-7 Icaa CPG |
| ssR-Me Amino Linker |
| ssR-H Amino Linker |
| C: Spacer Modifiers and Supports |
|---|
| Butyl CPG (for 3’-Butanol Spacer) |
| DMTr-Butane-diol phosphoramidite; C4 Spacer |
| DMTr-Butanol polystyrene |
| DMTr-C3 lcaa CPG |
| DMTr-Dodecane-diol phosphoramidite; C12 Spacer |
| DMTr-Ethane-diol phosphoramidite; C2 Spacer |
| DMTr-Hexa-Ethyloxy-glycol phosphoramidite; Spacer 18 |
| DMTr-Hexane-diol phosphoramidite; C6 Spacer |
| DMTr-Nonane-diol phosporamidite; C9 Spacer |
| DMTr-Propane-diol phosphoramidite; C3 Spacer |
| DMTr-Tetraethyloxy-glycol phosphoramidite; Spacer 12 |
| DMTr-Triethyloxy-glycol phosphoramidite; Spacer 9 |
| Glyceryl CPG; 1-DMTr-2-OAc-Glyceryl Succinate attached to lcaa CPG |
| Palmitoyl polystyrene support |
| Pentane diol; C5 Spacer |
| Polyethyleneglycol 2000 CEP |
| Polyethylenglycol 4500 CEP, (Avg. M.W.~4500) |
| Polyethylenglycol 4500 CEP, (Avg. M.W.~4500) |
| TOM C6 CEP |
| D: Thiol Modifiers and Supports |
|---|
| 3'-Thiol-modifiers-C3-S-S CPG |
| 5'-Thiol (trityl)-modifiers-C6 CEP, 5’-Thiol (trityl)-modifiers-C6 CPG |
| 5'-Thiol (trityl)-modifiers-dC ( N -Ac) CEP |
| 5'-Thiol C3 SS modifier |
| 5'-Thiol-modifiers C6 CEP; 1-Thiobenzoyl-hexanol-6 Cyanoethyl CEP |
| Thiol-modifiers-C6-S-S CEP |
| Thiol-modifiers-C6-S-S CPG; DMTr-C6-Disulfide Solid Support |
| Cyclic Dithiolane Disulfide Phosphoramidite, Cyclic Dithiolane Disulfide lcaa CPG |
| E: Branching phosphoramidites and Supports |
|---|
| Asymmetrical Branching CEP |
| Asymmetrical Branching lcaa CPG |
| NPPOC-DMT Asymmetrical Branching CEP |
| Symmetrical Branching CEP |
| Symmetrical Branching Polystyrene Support |
| Symmetrical Branching Support lcaa CPG |
Labelling
| A: Biotin Labelling |
|---|
| Biotin (BB) -TEG CEP |
| Biotin (BB)TM CEP |
| Biotin (BB)TM CPG; D-(+) Biotin lcaa CPG |
| Biotin dT CEP |
| Biotin TEG lcaa CPG |
| B: Psoralen labelling |
|---|
| Psoralen C-18 Hexaethyloxyglycol CEP |
| Psoralen C-2 CEP |
| Psoralen C-4 CEP |
| Psoralen C-4 lcaa CPG |
| Psoralen C-6 CEP |
| Psoralen CEP |
| Psoralen Support |
| C: Fluorescein Labelling |
|---|
| 3'-Fluorescein-dT CPG |
| 3'-Fluorescein-Icaa CPG |
| 5-FAM CPG |
| 5-Fluorescein CEP |
| 5'-Fluorescein CPG, 6-FAM lcaa CPG (DMTr) |
| 5'-Fluorescein-phosphoramidite; 6-FAM |
| 5'-Hexachloro-fluorescein Phosphoramidite |
| 5'-Tetrachloro-fluorescein Phosphoramidite |
| 6-FAM CPG |
| DMTr-5-FAM Phosphoramidite |
| DMTr-6-FAM Phosphoramidite |
| Etheno-adenosine 2'/3'-lcaa CPG |
| Etheno-adenosine 2'-TBDMS 3'-lcaa CPG |
| Etheno-adenosine CEP; Etheno-2'-O-TBDMS Adenosine CEP |
| Etheno-cytidine 2'/3'-lcaa CPG |
| Etheno-cytidine 2’-TBDMS 3’-lcaa CPG |
| Etheno-cytidine CEP; Etheno-2'-O-TBDMS Cytidine CEP |
| Etheno-deoxyadenosine 3'-lcaa CPG |
| Etheno-deoxyadenosine CEP |
| Etheno-deoxycytidine 3'-lcaa CPG |
| Etheno-deoxycytidine CEP |
| Fluorescein dT CEP |
| Hexachloro-fluorescein Cyclohexyl CEP |
| Tetrachloro-fluorescein Cyclohexyl CEP |
| D: DNP Labelling |
|---|
| DNP-TEG CEP |
| DNP-TEG-lcaa-CPG |
| Naphthyl Red phosphoramidite |
| E: Rhodamine (Tamra) labelling |
|---|
| 5'-TAMRA CEP |
| 6-TAMRA Supports & Columns |
| Cocktail for TAMRA |
| N 4-6-TAMRA Cytidine-3’- lcaa CPG |
| Rox Supports & Columns |
| F: Dabcyl Labelling |
|---|
| 3'-Dabcyl CEP |
| 3'-Dabcyl CPG |
| Dabcyl Succinyl diamido Polystyrene Support |
| Dabsyl-dT CEP |
| G: Cholesterol |
|---|
| Cholesterol (Plant) TEG CEP |
| Cholesterol (TEG) CEP (Will Generate DMT color) |
| Cholesterol C4 lcaa CPG |
| Cholesterol TEG lcaa CPG |
| TEG Cholesterol (Tetra Ethylene Glycol) CEP |
| TEG Cholesterol (Tetra Ethylene Glycol) Phosphonate |
| TEG Cholesterol (Tetra Ethylene Glycol) -H- Phosphonate TEA Salt\" |
| H: Tocopherol |
|---|
| (+)- a -Tocopherol-Phosphoramidite |
| I: Carboxyl Generating reagents |
|---|
| 5'-Carboxyl Group Generating Reagent; Thymidine-Succinyl Hexamide CEP |
| C-5 Carboxy Introducing Amidite; Deoxy Uridine 5-Methylacrylate CEP |
| Hexane-6-Carboxyamido (p-benzaldehyde)- 1-ol CEP |
| Undecanoic CEP |
| J: Puromycin Labelling |
|---|
| 3'-L-(O-Methyl)-Tyrosine-puromycin-2'- Icaa CPG |
| Puromycin-2'-Succinyl Icaa CPG |
| A: Chemical Phosphorylation and Supports |
|---|
| 5'- Phosphorylation; Bis-cyanoethyl- N , N -diisopropyl CEP |
| 3'-Phosphate-ON Icaa CPG |
| 5-Phosphorylation (generates DMTr color)-(5-Phosphate-ON Reagent) |
| B: Amino Modifiers and Supports |
|---|
| 3'-Amino (Fmoc)-modifier DMTr CEP |
| 3'-Amino (Fmoc)-modifier-C3 CPG |
| 3'-Amino (Fmoc)-modifier-C7 CPG |
| 3'-Amino (TFA)-modifier-spacer 6 Polystyrene |
| 3'-Amino (TFA)-modifier-spacer 7-Icaa CPG |
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