A guide to recent and growing trends in protein tags
Protein tagging has been around for decades, but their traditional uses are not frequent enough to make them common lab knowledge. Recent improvements in biochemical techniques have been increasing their frequency as creative design and analysis tools. In this overview, we offer a guide for the variety of different protein tags that are trending in recent growth.
This guide will cover:
Overview | |
---|---|
Function | Immobilize functional proteins on flat surface |
Application | Studying interactions with ligands |
Feature | GFP with Arg-tag can be bound to mica surfaces |
Application | Scanning probe microscopy applications |
Terminus Attachment | C-term |
Compatible Domain | Bacterial |
Measured Biochemistry | |
---|---|
Purity | 95% |
Yield | 44% |
Overview | |
---|---|
Function | Immobilization, linear recognition motif |
Application | Purification (most common method) |
Feature | Strongest interaction with immobilized metal ion matrice |
Application | Immobilized metal-affinity chromatography (IMAC) |
Terminus Attachment | N or C-terminal |
Compatible Domain | Bacterial; Yeast; Mammalian; Viral |
Measured Biochemistry | |
---|---|
Purity | 95% |
Yield | 44% |
Overview | |
---|---|
Function | Increased solubility, highly antigenic |
Application | Purification |
Feature | Amplified detection |
Application | Protein expression |
Terminus Attachment | N or C-terminal |
Overview | |
---|---|
Function | Matrix regenerable, recognition motif |
Application | Purification and detection |
Feature | Eukaryotic cell surface display, surface immobilization |
Application | SPR CHIPS, NMR, Crystallization, Protein interactions |
Terminus Attachment | N or C-terminal |
Compatible Domain | Bacterial; Yeast; Mammalian; Insects; Plants; Synthetic |
Overview | |
---|---|
Function | Increase proteolytic stability, high affinity immobilization |
Application | Detection and Expression support |
Feature | Newer low affinity versions |
Application | Purification |
Terminus Attachment | N or C-terminal |
Compatible Domain | Bacterial; Yeast; Mammalian; Insects; Plants; Synthetic |
Overview | |
---|---|
Function | 9E10 immunological recognition |
Application | Immunoblotting/WB, Immunoprecipitation, Flow Cytometry |
Feature | Convertible Reagent |
Application | Protein engineering |
Terminus Attachment | N or C-terminal |
Compatible Domain | Bacterial; Yeast; Mammalian; Insects |
Overview | |
---|---|
Function | Peptide bound to antibody (M1) |
Application | Purification |
Feature | Consistent non-denaturing conditions |
Application | Purifying active fusion proteins |
Terminus Attachment | N or C-terminal |
Compatible Domain | Bacterial; Yeast; Mammalian |
Measured Biochemistry | |
---|---|
Purity | 90% |
Overview | |
---|---|
Function | Viral antibody recognition |
Application | Detection |
Feature | Anti-HA antibodies specific |
Application | Expression |
Terminus Attachment | N or C-terminal |
Compatible Domain | Mammalian |
Measured Biochemistry | |
---|---|
Yield | Low |
Overview | |
---|---|
Function | Luminescent dye |
Application | Detection |
Feature | Serve as a reporter immediately upon translation |
Application | In-situ hybridization, RNA processing, RNA transfection |
Terminus Attachment | N-terminal |
Compatible Domain | Bacterial; Yeast; Mammalian; Insects |
Overview | |
---|---|
Function | Intrinsic fluorescence can permit native detection |
Application | Detection |
Feature | No antibody needed for detection |
Application | Monitor gene expression, protein folding, and targeting |
Terminus Attachment | N-terminal |
Compatible Domain | Bacterial; Yeast; Mammalian; Insects |
Overview | |
---|---|
Function | Specific linear recognition motif |
Application | Detection |
Feature | RNase S specificity |
Application | Diverse assays; quantitative, colorimetric no antibody |
Terminus Attachment | N or C-terminal |
Compatible Domain | Bacterial; Yeast; Mammalian |
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