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Reagents for Nucleic Acid Chemistry
L L • November 24, 2017
Reagents for Nucleic Acid Chemistry
See the full list of in-house Reagents used in our synthesis
Optimized Nucleotide Synthesis
Our nucleotide synthesis is modeled to introduce technical & chemical optimization from start to finish. We achieve that by utilizing our precision arsenal of formulations to aid & improve very specific moments throughout our solid-phase production. Activation, sulfurizing, phosphorylating and capping reagents help add an extra layer of customization to every project we synthesize. NHS Esters enhance affinity and fluorescence and our mono-,di-, triphosphates offer more control to certain chemical reactions.
Reagents for nucleic acid chemistry
| A: Activation Reagents |
|---|
| 1 H -1,2,4-Triazole, (1,2,4-Triazole) |
| 1 H -Imidazole-4,5-dicarbonitrile, (Dicyanoimidazole (DCI)) |
| 5-Benzyl-thio-1-H-tetrazole (BMT) |
| 5-Ethyl-thio-1-H-tetrazole (ETT) |
| Diisopropyl-ammonium-1 H -tetrazolide |
| Dimethoxytrityl-Chloride; DMTr-Chloride |
| Mesitylene Sulphonyl-3-Nitro-1,2,4-Triazole (MSNT)) |
| Monoimethoxytrityl-Chloride; MMTr-Chloride |
| Trimethoxytrityl-Chloride; TMTr-Chloride |
| B: Beaucage Reagents & Sufurizing reagents |
|---|
| Phenylacetyl-Disulfide (PADS) |
| Silanized Bottle (for Beaucage Reagent) |
| DDTT ((Dimethylamino-methylidene)amino)-3 H -1,2,4-dithiazoline-3-thione / Sulfurizing Reagent |
| 3 H -1,2-Benzodithiol-3-one-1,1-dioxide/ Sulfurizing Reagent / Sulfurizing Reagent (Beaucage Reagent) |
| C: Phosphorylating Reagents |
|---|
| Dichloromethoxy-phosphite |
| 2-Cyanoethyl- N , N , N , N -teraisopropylphosphane |
| Bis (diisopropylamino)-chlorophosphine |
| Cyanoethyl-dichlorophosphite |
| Diethoxy- N , N -diisopropyl-phosphoramidite |
| Diethyl-phosphorochloride |
| Ethylene-glycol-morpholino phosphoramidite |
| Methoxy- N , N , N , N -tetraisopropylphosphane |
| N , N -Diisopropylamino-Cyanoethyl phosphonamidic-chloride |
| N , N -Diisopropylamino-methyl-phosphonamidic-chloride |
| N , N -Diisopropyl-methoxy-phosphonamidic-Cl |
| O -Chlorophenyl-phosphorodichloridate |
| P -Ethoxy-Chloro-Reagent |
| D: Nucleoside Synthesis Reagents |
|---|
| 4-Isopropyl-Phenoxy-Acetyl-Chloride |
| Bis-Silyating Reagent |
| E: Sugars, Purines & Pyrimidines |
|---|
| 1,3,5-Tri – O -acetyl-2-deoxy- β -L-ribose |
| 1,3,5-Tri– O -acetyl-2-deoxy- β -D-ribose |
| 1-Alpha-Chloro-3,5-ditoluoyl-2-deoxy- β -D-ribose |
| 1- O -Methyl-2,3,5-tri- O -benzoyl- β -D-ribose |
| 2,3,5- O -Tribenzoyl- β -D-Ribofuranosyl-1-Acetate |
| 2-Deoxy- β -D-ribose |
| 2-Deoxy- β -L-ribose |
| 6-Chloro-7-deaza-Guanine |
| 6-Chloro-7-deaza-Purine |
| 6-Chloro-Guanine |
| 6-Chloro-Purine |
NHS ester/Monophosphate/Diphosphate: NHS esters
| A: Fluorescein |
|---|
| 5-Fluorescein-Dipivalate-NHS Ester (5-FAM Dipivalate NHS Ester)\" |
| 5-Fluorescein-NHS Ester(5-FAM NHS Ester) |
| 6-Fluorescein-Dipivalate-NHS Ester (6-FAM Dipivalate NHS Ester) |
| 6-Fluorescein-NHS Ester (6-FAM NHS Ester) |
| B: Biotin |
|---|
| Biotin-Amido-Caproate- N -Hydroxy |
| Biotin-Ester ( N -Hydroxysuccinimide) |
| Biotin-X-Y-SSE (Biotinamidohexanoyl-amidobutanoyl Succinimidyl ester) |
Monophosphates
| A: Deoxy N -Alkylated monophosphates |
|---|
| N 1 -Methyl-deoxyadenosine-5’-monophosphate |
| N 3 -Methyl-deoxycytidine-5’-monophosphate |
| N 6 -Methyl-deoxyadenosine-5’-monophosphate |
| B: Etheno deoxy & ribo monophosphates |
|---|
| Etheno-deoxycytidine-5’-monophosphate |
| Etheno-ribocytidine-5’-monophosphate |
| C: 7-Deaza deoxymonophosphate |
|---|
| 7-Deaza-deoxyadenosine-5’-monophosphate |
| D: 2’- O -Methyl-ribo monophosphates |
|---|
| 2’- O -Methyl-adenosine ( N 6 -Benzyl)-5’-monophosphate |
| 2’- O -Methyl-cytidine-5’-monophosphate |
| 2’- O -Methyl-guanosine-5’-monophosphate |
| E: β -L-ribo monophosphates |
|---|
| β -L-Adenosine-5’-monophosphate sodium salt |
| β -L-Uridine-5’-monophosphate sodium salt |
| F: 3'-Substituted-ribo monophosphates |
|---|
| 3'-Amino-guanosine-5’-monophosphate |
| 3'-Methyl-guanosine-5’-monophosphate |
| G: ribo monophosphate |
|---|
| Thymidine-5’-monophosphate |
| H: Iso ribo monophosphate |
|---|
| Iso-guanosine ( N -iBu)-5’-monophosphate |
Diphosphates
| A: 2'- O -Methyl ribo 3’,5’-diphosphates |
|---|
| 2'- O -Methyl-adenosine-3’,5’-diphosphate disodium salt |
| 2'- O -Methyl-cytidine-3’,5’-diphosphate disodium salt |
| 2'- O -Methyl-guanosine-3’,5’-diphosphate disodium salt |
| 2'- O -Methyl-uridine-3’,5’-diphosphate disodium salt |
| B: Ribo U 5'-diphoosphates |
| Uridine-5’-diphosphate |
| Uridine-5’-diphosphate disodium salt |
Triphosphates
| A: Etheno deoxy 5’-triphosphates |
|---|
| Etheno-deoxyadenosine-5’-triphosphate disodium salt |
| Etheno-deoxycytidine-5’-triphosphate disodium salt |
| B: 7-Deaza-deoxy 5’-triphosphates |
|---|
| 7-Deaza-deoxyadenosine-5’-triphosphate disodium salt |
| 7-Deaza-deoxyguanosine-5’-triphosphate disodium salt |
| C: Iso deoxy Triphosphate |
|---|
| Iso-deoxyguanosine-5’-triphosphate disodium salt |
| D: 7-Deaza-7-Propargylamine, dideoxy Triphosphates |
|---|
| 7-Deaza-7-propargylamine-2',3'-dideoxyadenosine-5’-triphosphate |
| 7-Deaza-7-propargylamine-2',3'-dideoxyadenosine-5’-triphosphate disodium salt |
| 7-Deaza-7-propargylamine-2',3'-dideoxyguanosine-5’-triphosphate disodium salt |
| E: 5-Propargyl amine-2',3'-Dideoxy Triphosphates |
|---|
| 5-Propargylamine-2',3'-dideoxycytidine-5’-triphosphate |
| 5-Propargylamine-2',3'-dideoxycytidine-5’-triphosphate disodium salt |
| 5-Propargylamine-2',3'-dideoxyuridine-5’-triphosphate disodium salt |
| F: 2',3'-Dideoxy G Triphosphate |
|---|
| 2',3'-Dideoxyguanosine-5’-triphosphate |
| G: 2'- O -Methyl-ribo 5’-triphosphates |
|---|
| 2'- O -Methyl-adenosine-5’-triphosphate disodium salt |
| 2'- O -Methyl-cytidine-5’-triphosphate disodium salt |
| 2'- O -Methyl-guanosine-5’-triphosphate disodium salt |
| 2'- O -Methyl-uridine-5’-triphosphate disodium salt |
| H: Ara-ribo Triphosphates |
|---|
| Ara-adenosine-5’-triphosphate disodium salt |
| Ara-cytidine-5’-triphosphate disodium salt |
| Ara-guanosine-5’-triphosphate disodium salt |
| Ara-uridine-5’-triphosphate disodium salt |
| I: Ara-2'- O -Methyl-ribo Triphosphates |
|---|
| Ara-(2'- O -Methyl)-adenosine-5’-triphosphate disodium salt |
| Ara-(2'- O -Methyl)-cytidine-5’-triphosphate disodium salt |
| Ara-(2'- O -Methyl)-guanosine-5’-triphosphate disodium salt |
| Ara-(2'- O -Methyl)-uridine-5’-triphosphate disodium salt |
| J: Iso ribo triphosphate |
|---|
| Iso-Guanosine-5’-triphosphate disodium salt |
Solvents/Reagents-DNA Synthesis
| A: PolyPlex Oligo-Synthesizer |
|---|
| Acetonitrile Anhydrous (Phosphoramidite Diluent & Washings), PolyPlex Oligonucleotide Synthesizer |
| Acetonitrile Wash Grade,PolyPlex Oligonucleotide Synthesizer |
| Activation Reagent (0.25M 4,5-Dicyanoimidazole/Acetonitrile), PolyPlex Oligonucleotide Synthesizer |
| Activation Reagent (0.25M 5-Ethylthio Tetrazole/Acetonitrile), PolyPlex Oligonucleotide Synthesizer |
| Activation Reagent (0.45M 5-Ethylthio Tetrazole/Acetonitrile), PolyPlex Oligonucleotide Synthesizer |
| Activation Reagent (BMT Solution) (0.3M Benzylthio Tetrazole/Acetonitrile) PolyPlex Oligonucleotide Synthesizer |
| AMA Reagent, PolyPlex Oligonucleotide Synthesizer |
| CAP A (Acetic Anhydride/Pyridine/THF),PolyPlex Oligonucleotide Synthesizer |
| CAP B (10% N -Methylimidazole/THF),PolyPlex Oligonucleotide Synthesizer |
| CAP B (16% N -Methylimidazole/THF), PolyPlex Oligonucleotide Synthesizer |
| DDTT Solution 1 [0.05M] (Sulfurizing Reagent), PolyPlex Oligonucleotide Synthesizer |
| DDTT Solution 1 [0.10M] (Sulfurizing Reagent), PolyPlex Oligonucleotide Synthesizer |
| DMTr Removal Reagent (3% Dichloracetic Acid/Dichloromethane), PolyPlex Oligonucleotide Synthesizer |
| DMTr Removal Reagent (3% Trichloracetic Acid/Dichloromethane), PolyPlex Oligonucleotide Synthesizer |
| Oxidation Solution (0.02M Iodine/Pyridine/H2O/THF), PolyPlex Oligonucleotide Synthesizer |
| Oxidation Solution (0.1M Iodine/Pyridine/H2O/THF), PolyPlex Oligonucleotide Synthesizer |
| Silanized Bottles for Beaucage Reagent, PolyPlex Oligonucleotide Synthesizer |
| Sulfurizing Reagent (Beaucage Reagent) for Introduction of Phosphorothioate (S-oligonucleotide), PolyPlex Oligonucleotide Synthesizer |
| Thiophenol/Triethylamine/Dioxane, PolyPlex Oligonucleotide Synthesizer |
| B: MerMade Oligo-Synthesizer |
|---|
| Acetonitrile Anhydrous (Phosphoramidite Diluent & Washings), MerMade Oligonucleotide Synthesizer |
| Acetonitrile Wash Grade, MerMade Oligonucleotide Synthesizer |
| Activation Reagent (0.25M 4,5-Dicyanoimidazole/Acetonitrile), MerMade Oligonucleotide Synthesizer |
| Activation Reagent (0.25M 5-Ethylthio Tetrazole/Acetonitrile), MerMade Oligonucleotide Synthesizer |
| Activation Reagent (0.45M 5-Ethylthio Tetrazole/Acetonitrile), MerMade Oligonucleotide Synthesizer |
| Activation Reagent (BMT Solution) (0.3M Benzylthio Tetrazole/Acetonitrile) MerMade Oligonucleotide Synthesizer |
| AMA Reagent, MerMade Oligonucleotide Synthesizer |
| CAP A (Acetic Anhydride/Pyridine/THF),MerMade Oligonucleotide Synthesizer |
| CAP B (10% N -Methylimidazole/THF), MerMade Oligonucleotide Synthesizer |
| CAP B (16% N -Methylimidazole/THF), MerMade Oligonucleotide Synthesizer |
| DDTT Solution 1 [0.05M] (Sulfurizing Reagent), MerMade Oligonucleotide Synthesizer |
| DDTT Solution 1 [0.10M] (Sulfurizing Reagent), MerMade Oligonucleotide Synthesizer |
| DMTr Removal Reagent (2.5% Dichloracetic Acid/Dichloromethane), MerMade Oligonucleotide Synthesizer |
| DMTr Removal Reagent (3% Trichloracetic Acid/Dichloromethane), MerMade Oligonucleotide Synthesizer |
| Oxidation Solution (0.02M Iodine/Pyridine/H2O/THF), MerMade Oligonucleotide Synthesizer |
| Oxidation Solution (0.1M Iodine/Pyridine/H2O/THF), MerMade Oligonucleotide Synthesizer |
| Silanized Bottles for Beaucage Reagent, MerMade Oligonucleotide Synthesizer |
| Sulfurizing Reagent (Beaucage Reagent) for Introduction of Phosphorothioate (S-oligonucleotide), MerMade Oligonucleotide Synthesizer |
| Thiophenol/Triethylamine/Dioxane, MerMade Oligonucleotide Synthesizer |
| C: Expedite Oligo-Synthesizer |
|---|
| Acetonitrile Anhydrous (Phosphoramidite Diluent & Washings), Expedite Oligonucleotide Synthesizer |
| Acetonitrile Wash Grade, Expedite Oligonucleotide Synthesizer |
| Activation Reagent (0.25M 4,5-Dicyanoimidazole/Acetonitrile), Expedite Oligonucleotide Synthesizer |
| Activation Reagent (0.25M 5-Ethylthio Tetrazole/Acetonitrile), Expedite Oligonucleotide Synthesizer |
| Activation Reagent (0.45M 5-Ethylthio Tetrazole/Acetonitrile), Expedite Oligonucleotide Synthesizer |
| Activation Reagent (BMT Solution) (0.3M Benzylthio Tetrazole/Acetonitrile) Expedite Oligonucleotide Synthesizer |
| AMA Reagent, Expedite Oligonucleotide Synthesizer |
| CAP A (Acetic Anhydride/Pyridine/THF), Expedite Oligonucleotide Synthesizer |
| CAP B (10% N -Methylimidazole/THF), Expedite Oligonucleotide Synthesizer |
| CAP B (16% N -Methylimidazole/THF), Expedite Oligonucleotide Synthesizer |
| DMTr Removal Reagent (2.5% Dichloracetic Acid/Dichloromethane), Expedite Oligonucleotide Synthesizer |
| DMTr Removal Reagent (3% Trichloracetic Acid/Dichloromethane), Expedite Oligonucleotide Synthesizer |
| Oxidation Solution (0.02M Iodine/Pyridine/H2O/THF), Expedite Oligonucleotide Synthesizer |
| Oxidation Solution (0.1M Iodine/Pyridine/H2O/THF), Expedite Oligonucleotide Synthesizer |
| Silanized Bottles for Beaucage Reagent, Expedite Oligonucleotide Synthesizer |
| Sulfurizing Reagent (Beaucage Reagent) for Introduction of Phosphorothioate (S-oligonucleotide), Expedite Oligonucleotide Synthesizer |
| Thiophenol/Triethylamine/Dioxane, Expedite Oligonucleotide Synthesizer |
| D: Expedite Oligo-Synthesizer |
|---|
| Activator (Benzyl thio tetrazole; 0.2M in acetonitrile) Akta Oligonucleotide Synthesizer |
| Cap A ( N -Methyl imidazole; 20% in Acetonitrile) Akta Oligonucleotide Synthesizer |
| Cap B1 (Acetic Anhydride; 40% (v:v) in Acetonitrile) Akta Oligonucleotide Synthesizer |
| Cap B2 (Symmetrical Collidine; 60% (v:v) in Acetonitrile, Akta Oligonucleotide Synthesizer |
| Deblock (3% DCA in Toluene) Akta Oligonucleotide Synthesizer |
| Oxidizer 4 Liter 50 mM Iodine in 10% Water/Pyridine) Akta Oligonucleotide Synthesizer |
| E: ABI 3900 Oligo-Synthesizer |
|---|
| Acetonitrile Anhydrous (Phosphoramidite Diluent & Washings), ABI 3900 Oligonucleotide Synthesizer |
| Acetonitrile Wash Grade, ABI 3900 Oligonucleotide Synthesizer |
| Activation Reagent (0.25M 4,5-Dicyanoimidazole/Acetonitrile), ABI 3900 Oligonucleotide Synthesizer |
| Activation Reagent (0.25M 5-Ethylthio Tetrazole/Acetonitrile) |
| Activation Reagent (0.45M 5-Ethylthio Tetrazole/Acetonitrile), ABI 3900 Oligonucleotide Synthesizer |
| Activation Reagent (BMT Solution) (0.3M Benzylthio Tetrazole/Acetonitrile) |
| AMA Reagent, ABI 3900 Oligonucleotide Synthesizer |
| CAP A (Acetic Anhydride/Pyridine/THF), ABI 3900 Oligonucleotide Synthesizer |
| CAP B (10% N -Methylimidazole/THF), ABI 3900 Oligonucleotide Synthesizer |
| CAP B (16% N -Methylimidazole/THF), ABI 3900 Oligonucleotide Synthesizer |
| DDTT Solution 1 [0.05M] (Sulfurizing Reagent), ABI 3900 Oligonucleotide Synthesizer |
| DDTT Solution 1 [0.10M] (Sulfurizing Reagent), ABI 3900 Oligonucleotide Synthesizer |
| DMT Removal Reagent (3% Dichloracetic Acid/Dichloromethane), ABI 3900 Oligonucleotide Synthesizer |
| DMT Removal Reagent (3% Trichloracetic Acid/Dichloromethane) |
| Oxidation Solution (0.02M Iodine/Pyridine/H2O/THF), ABI 3900 Oligonucleotide Synthesizer |
| Oxidation Solution (0.1M Iodine/Pyridine/H2O/THF), ABI 3900 Oligonucleotide Synthesizer |
| Silanized Bottles for Beaucage Reagent, ABI 3900 Oligonucleotide Synthesizer |
| Sulfurizing Reagent (Beaucage Reagent) for Introduction of Phosphorothioate (S-oligonucleotide), ABI 3900 Oligonucleotide Synthesizer |
| Thiophenol/Triethylamine/Dioxane, ABI 3900 Oligonucleotide Synthesizer |
| F: ABI Oligo-Synthesizer |
|---|
| Acetonitrile Anhydrous (Phosphoramidite Diluent & Washings), ABI Oligonucleotide Synthesizer |
| Acetonitrile Wash Grade, ABI Oligonucleotide Synthesizer |
| Activation Reagent ((0.25M 5-Ethylthio Tetrazole/Acetonitrile), ABI Oligonucleotide Synthesizer |
| Activation Reagent (0.25M 4,5-Dicyanoimidazole/Acetonitrile), ABI Oligonucleotide Synthesizer |
| Activation Reagent (0.45M 5-Ethylthio Tetrazole/Acetonitrile), ABI Oligonucleotide Synthesizer |
| Activation Reagent (BMT Solution) (0.3M Benzylthio Tetrazole/Acetonitrile), ABI Oligonucleotide Synthesizer |
| AMA Reagent, ABI Oligonucleotide Synthesizer |
| CAP A (Acetic Anhydride/Pyridine/THF), ABI Oligonucleotide Synthesizer |
| CAP B (10% N-Methylimidazole/THF), ABI Oligonucleotide Synthesizer |
| CAP B (16% N-Methylimidazole/THF), ABI Oligonucleotide Synthesizer |
| DMT Removal Reagent (3% Dichloracetic Acid/Dichloromethane), ABI Oligonucleotide Synthesizer |
| DMT Removal Reagent (3% Trichloracetic Acid/Dichloromethane), ABI Oligonucleotide Synthesizer |
| Oxidation Solution (0.02M Iodine/Pyridine/H2O/THF), ABI Oligonucleotide Synthesizer |
| Oxidation Solution (0.1M Iodine/Pyridine/H2O/THF), ABI Oligonucleotide Synthesizer |
| Silanized Bottles for Beaucage Reagent, ABI Oligonucleotide Synthesizer |
| Sulfurizing Reagent (Beaucage Reagent) for Introduction of Phosphorothioate (S-oligonucleotide), ABI Oligonucleotide Synthesizer |
| Thiophenol/Triethylamine/Dioxane, ABI Oligonucleotide Synthesizer |
| G: Solvents/Reagents For H -phosphonate Chemistry |
|---|
| Acetonitrile/Pyridine (50:50), Anhydrous (Diluent for Monomers) |
| Acetonitrile/Pyridine (95:5), Anhydrous (Diluent for Activator) |
| Acetonitrile/Pyridine (wash solvent) |
| Activator for monomers and Capping Reagent (Adamantane catbonyl chloride) |
| Capping for ABI Synthesizers (Isopropyl Phosphite/Acetonitrile/Pyridine) |
| Dipentaflurophenyl Carbonate (Reagent for H-phosphonate) |
| Oxidation Solution 1 (Iodine in Pyridine/H2O/THF) |
| Oxidation Solution 2 (THF/H2O/TEA) |
| H: Deprotection solution for DNA Synthesis |
|---|
| Ammonia Free Oligo Deprotection Solution |
| Deprotection solution for Levulinyl |
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