Recombinant Yeast inorganic Pyrophosphatase is an essential enzyme for hydrolyzing inorganic pyrophosphate during in vitro transcription of mRNA. Incorporating pyrophosphate in the reaction, the mRNA yield dramatically increases. We offer high quality pyrophosphatase suitable for lab-scale as well as industrial-scale applications.

Advantages:

• Manufactured in accordance with GMP rules and regulations
• 100% animal origin free manufacturing process
• Endotoxin and antibiotic free
• Complete QA documentation available

• Manufacture of mRNA vaccines
• Manufacture of mRNA therapeutics

EC Number:

3.6.1.1

Source:

Recombinant E.Coli

Molecular Weight:

34 kD

Cofactors:

Mg2+

Yeast inorganic Pyrophosphatase is expressed with an N-terminal His-Tag in Recombinant E.coli

Appearance:

Colorless to yellowish solution

Volume Activity:

NLT 600 U/mL

Unit Definition:

One unit of the Pyrophosphatase catalyzes the hydrolysis of 1 μmol inorganic pyrophosphate in 1 min at 25°C

Protein (OD280):

1.8-2.2 mg/mL

Purity:

NLT 95 %

Nonspecific Endocleases:

Not detectable

Nicking Activity:

Not detectable

RNase Activity:

Not detectable

Exonucleases:

Not detectable

Proteases:

Not detectable

Endotoxins:

NMT 10 EU/mL

Total Bioburden:

NMT 10 CFU/mL

E.coli Host Cell DNA:

NMT 50 ppm

E.coli Host Cell Protein:

NMT 10 ppm

Stability:

-15 to -25°C within specification range for 36 months from date of manufacture.

Storage:

Enzyme supplied in storage buffer with composition:

20 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 8 @ 25°C

Unit Definition:

One unit is the amount of enzyme that will generate 1 μmol of phosphate per minute from inorganic pyrophosphate under standard reaction conditions.

Notes on use:

• 1–3 units per ml pyrophosphatase should be added for a high yield in-vitro transcription reaction.
• Pyrophosphatase works well in any buffer containing Mg++ (1-10 mM)

Method of Analysis will be provided on Bulk Enzyme orders for customer’s in-house quality testing

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