APCS
Human Recombinant APCS - His Tagged
Reference ID:KB-3939
Western Blot
ELISA
FACS
Protein:Protein
Flow Cytometry
Gene of Interest
Gene Synonyms:APCS;PTX2
Protein Names:Serum amyloid P-component (SAP) (9.5S alpha-1-glycoprotein) [Cleaved into: Serum amyloid P-component(1-203)]
Accession Data
Organism:Homo sapiens (Human)
Mass (kDa):253.87
Length (aa):223
Sequence:MNKPLLWISVLTSLLEAFAHTDLSGKVFVFPRESVTDHVNLITPLEKPLQNFTLCFRAYSDLSRAYSLFSYNTQGRDNELLVYKERVGEYSLYIGRHKVTSKVIEKFPAPVHICVSWESSSGIAEFWINGTPLVKKGLRQGYFVEAQPKIVLGQEQDSYGGKFDRSQSFVGEIGDLYMWDSVLPPENILSAYQGTPLPANILDWQALNYEIRGYVIIKPLVWV
Proteomics (Proteome ID):UP000005640
Proteomics (Chromosome): Chromosome 1
Mass Spectrometry: Mass=25462.5; Mass_error=1.1; Method=Electrospray; Range=20-223; Evidence={ECO:0000269|PubMed:15174148, ECO:0000269|PubMed:8202534}; Mass=25463; Mass_error=3; Method=MALDI; Range=20-223; Evidence={ECO:0000269|PubMed:15174148, ECO:0000269|PubMed:8202534}; Mass=1285.78; Method=MALDI; Range=213-223; Evidence={ECO:0000269|PubMed:15174148}; Mass=1186.71; Method=MALDI; Range=213-222; Evidence={ECO:0000269|PubMed:15174148};
Cofactor:Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Note=Binds 2 calcium ions per subunit.;
Function [CC]:Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. May also function as a calcium-dependent lectin.
Metal Binding:METAL 77 77 Calcium 1. {ECO:0000255|PROSITE-ProRule:PRU01172}.; METAL 78 78 Calcium 1. {ECO:0000255|PROSITE-ProRule:PRU01172}.; METAL 155 155 Calcium 1. {ECO:0000255|PROSITE-ProRule:PRU01172}.; METAL 155 155 Calcium 2. {ECO:0000255|PROSITE-ProRule:PRU01172}.; METAL 156 156 Calcium 1; via carbonyl oxygen. {ECO:0000255|PROSITE-ProRule:PRU01172}.; METAL 157 157 Calcium 1. {ECO:0000255|PROSITE-ProRule:PRU01172}.; METAL 157 157 Calcium 2. {ECO:0000255|PROSITE-ProRule:PRU01172}.; METAL 167 167 Calcium 2. {ECO:0000255|PROSITE-ProRule:PRU01172}.
Tissue Specificity:Found in serum and urine. {ECO:0000269|PubMed:15174148}.
Chemical Profile | ChEBI:Ca(2+) [CHEBI:29108]
ChEBI (Cofactor):Ca(2+) [CHEBI:29108]
Disease:Note=SAP is a precursor of amyloid component P which is found in basement membrane and associated with amyloid deposits.
Reagent Data
Name:APCS
Subcategory:Protein
Region:His 20 - Val 223
Molecular Weight:24
Source:HEK293
Species:Human
Tag:His
Format:Lyophilized
Purification System:Chromatography
Formulation:Sterile-filtered colorless solution
Formulation Concentration:1 mg/ml
Buffer Volume:Standard
Buffer Solution:PBS
pH:7.4-7.5
Toxicity
Endotoxin Level:< 1%
Aggregate Tested By:SDS-PAGE
Endotoxin Screened:< 0.1 ng/ug
Purity:> 98%
Determined: SDS-PAGE
Stain:Blue
Chromotography:Anion-exchange
Validated: RP-HPLC
Sample Handling
Storage:-20°C
Stability:This bioreagent is stable at 4°C (short-term) and -70°C(long-term). After reconstitution, sample may be stored at 4°C for 2-7 days and below -18°C for future use.
Preparation:Reconstitute in sterile distilled H2O to no less than 100 ug/ml; dilute reconstituted stock further in other aqueous solutions if needed. Please review COA for lot-specific instructions. Final measurements should be determined by the end-user for optimal performance.