PH20
Human Recombinant SPAM1 - His Tagged
Reference ID:KB-3933
Western Blot
ELISA
FACS
Protein:Protein
Flow Cytometry
Gene of Interest
Gene Synonyms:SPAM1;HYAL3;PH20
Protein Names:Hyaluronidase PH-20 (Hyal-PH20) (EC 3.2.1.35) (Hyaluronoglucosaminidase PH-20) (Sperm adhesion molecule 1) (Sperm surface protein PH-20)
Accession Data
Organism:Homo sapiens (Human)
Mass (kDa):578.48
Length (aa):509
Sequence:MGVLKFKHIFFRSFVKSSGVSQIVFTFLLIPCCLTLNFRAPPVIPNVPFLWAWNAPSEFCLGKFDEPLDMSLFSFIGSPRINATGQGVTIFYVDRLGYYPYIDSITGVTVNGGIPQKISLQDHLDKAKKDITFYMPVDNLGMAVIDWEEWRPTWARNWKPKDVYKNRSIELVQQQNVQLSLTEATEKAKQEFEKAGKDFLVETIKLGKLLRPNHLWGYYLFPDCYNHHYKKPGYNGSCFNVEIKRNDDLSWLWNESTALYPSIYLNTQQSPVAATLYVRNRVREAIRVSKIPDAKSPLPVFAYTRIVFTDQVLKFLSQDELVYTFGETVALGASGIVIWGTLSIMRSMKSCLLLDNYMETILNPYIINVTLAAKMCSQVLCQEQGVCIRKNWNSSDYLHLNPDNFAIQLEKGGKFTVRGKPTLEDLEQFSEKFYCSCYSTLSCKEKADVKDTDAVDVCIADGVCIDAFLKPPMETEEPQIFYNASPSTLSATMFIVSILFLIISSVASL
Proteomics (Proteome ID):UP000005640
Proteomics (Chromosome): Chromosome 7
Active Site:ACT_SITE 148 148 Proton donor. {ECO:0000250}.
Catalytic Activity: Reaction=Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.; EC=3.2.1.35;
Function [CC]:Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid. {ECO:0000269|PubMed:8282124}.
Tissue Specificity:Testis. {ECO:0000269|PubMed:8234258}.
Mutagenesis:MUTAGEN 146 146 D->N: Reduces activity by 80%. {ECO:0000269|PubMed:9288901}.; MUTAGEN 148 148 E->Q: Loss of activity. {ECO:0000269|PubMed:9288901}.; MUTAGEN 211 211 R->G: Reduces activity by over 90%. {ECO:0000269|PubMed:9288901}.; MUTAGEN 284 284 E->Q: Loss of activity. {ECO:0000269|PubMed:9288901}.; MUTAGEN 287 287 R->T: Loss of activity. {ECO:0000269|PubMed:9288901}.
Reagent Data
Name:PH20
Subcategory:Enzyme
Region:Leu 36 - Tyr 482
Molecular Weight:52.1
Source:HEK293
Species:Human
Tag:His
Format:Lyophilized
Purification System:Chromatography
Formulation:Sterile-filtered colorless solution
Formulation Concentration:1 mg/ml
Buffer Volume:Standard
Buffer Solution:PBS
pH:7.4-7.5
Toxicity
Endotoxin Level:< 1%
Aggregate Tested By:SDS-PAGE
Endotoxin Screened:< 0.1 ng/ug
Purity:> 98%
Determined: SDS-PAGE
Stain:Blue
Chromotography:Anion-exchange
Validated: RP-HPLC
Sample Handling
Storage:-20°C
Stability:This bioreagent is stable at 4°C (short-term) and -70°C(long-term). After reconstitution, sample may be stored at 4°C for 2-7 days and below -18°C for future use.
Preparation:Reconstitute in sterile distilled H2O to no less than 100 ug/ml; dilute reconstituted stock further in other aqueous solutions if needed. Please review COA for lot-specific instructions. Final measurements should be determined by the end-user for optimal performance.