Mouse Recombinant Pcsk9 - His Tagged
Reference ID:KB-3931
Western Blot
Flow Cytometry
Gene of Interest
Gene Synonyms:Pcsk9;Narc1
Protein Names:Proprotein convertase subtilisin/kexin type 9 (EC 3.4.21.-) (Neural apoptosis-regulated convertase 1) (NARC-1) (Proprotein convertase 9) (PC9) (Subtilisin/kexin-like protease PC9)
Accession Data
Organism:Mus musculus (Mouse)
Mass (kDa):748.23
Length (aa):694
Proteomics (Proteome ID):UP000000589
Proteomics (Chromosome): Chromosome 4
Active Site:ACT_SITE 189 189 Charge relay system. {ECO:0000250}.; ACT_SITE 229 229 Charge relay system. {ECO:0000250}.; ACT_SITE 389 389 Charge relay system. {ECO:0000250}.
Activity Regulation: Its proteolytic activity is autoinhibited by the non-covalent binding of the propeptide to the catalytic domain. Inhibited by EGTA.
Cofactor:Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence={ECO:0000305};
Function [CC]:Crucial player in the regulation of plasma cholesterol homeostasis. Binds to low-density lipid receptor family members: low density lipoprotein receptor (LDLR), very low density lipoprotein receptor (VLDLR), apolipoprotein E receptor (LRP1/APOER) and apolipoprotein receptor 2 (LRP8/APOER2), and promotes their degradation in intracellular acidic compartments. Acts via a non-proteolytic mechanism to enhance the degradation of the hepatic LDLR through a clathrin LDLRAP1/ARH-mediated pathway. May prevent the recycling of LDLR from endosomes to the cell surface or direct it to lysosomes for degradation. Can induce ubiquitination of LDLR leading to its subsequent degradation. Inhibits intracellular degradation of APOB via the autophagosome/lysosome pathway in a LDLR-independent manner. Involved in the disposal of non-acetylated intermediates of BACE1 in the early secretory pathway. Inhibits epithelial Na(+) channel (ENaC)-mediated Na(+) absorption by reducing ENaC surface expression primarily by increasing its proteasomal degradation. Regulates neuronal apoptosis via modulation of LRP8/APOER2 levels and related anti-apoptotic signaling pathways. {ECO:0000269|PubMed:22481440, ECO:0000269|PubMed:22580899}.
Site:SITE 155 156 Cleavage; by autolysis. {ECO:0000250}.; SITE 221 222 Cleavage; by furin and PCSK5. {ECO:0000250}.
Developmental Stage:In the embryo, expressed in the liver at 9 dpc, in the skin and transiently in the telencephalon at 12 dpc, and in the kidney, small intestine and cerebellum at 15 dpc.
Induction:Down-regulated following a high-cholesterol diet. {ECO:0000269|PubMed:12897189}.
Tissue Specificity:Hepatocytes, kidney mesenchymal cells, intestinal ileum, colon epithelia and embryonic brain telencephalon neurons.
Chemical Profile | ChEBI:Ca(2+) [CHEBI:29108]
ChEBI (Cofactor):Ca(2+) [CHEBI:29108]
Reagent Data
Region:Gln 35 - Gln 694
Molecular Weight:72
Purification System:Chromatography
Formulation:Sterile-filtered colorless solution
Formulation Concentration:1 mg/ml
Buffer Volume:Standard
Buffer Solution:PBS
Endotoxin Level:< 1%
Aggregate Tested By:SDS-PAGE
Endotoxin Screened:< 0.1 ng/ug
Purity:> 98%
Determined: SDS-PAGE
Validated: RP-HPLC
Sample Handling
Stability:This bioreagent is stable at 4°C (short-term) and -70°C(long-term). After reconstitution, sample may be stored at 4°C for 2-7 days and below -18°C for future use.
Preparation:Reconstitute in sterile distilled H2O to no less than 100 ug/ml; dilute reconstituted stock further in other aqueous solutions if needed. Please review COA for lot-specific instructions. Final measurements should be determined by the end-user for optimal performance.