LTA4H
Human Recombinant LTA4H - His Tagged
Reference ID:KB-3926
Western Blot
ELISA
FACS
Protein:Protein
Flow Cytometry
Gene of Interest
Gene Synonyms:LTA4H;LTA4
Protein Names:Leukotriene A-4 hydrolase (LTA-4 hydrolase) (EC 3.3.2.6) (Leukotriene A(4) hydrolase)
Accession Data
Organism:Homo sapiens (Human)
Mass (kDa):692.85
Length (aa):611
Sequence:MPEIVDTCSLASPASVCRTKHLHLRCSVDFTRRTLTGTAALTVQSQEDNLRSLVLDTKDLTIEKVVINGQEVKYALGERQSYKGSPMEISLPIALSKNQEIVIEISFETSPKSSALQWLTPEQTSGKEHPYLFSQCQAIHCRAILPCQDTPSVKLTYTAEVSVPKELVALMSAIRDGETPDPEDPSRKIYKFIQKVPIPCYLIALVVGALESRQIGPRTLVWSEKEQVEKSAYEFSETESMLKIAEDLGGPYVWGQYDLLVLPPSFPYGGMENPCLTFVTPTLLAGDKSLSNVIAHEISHSWTGNLVTNKTWDHFWLNEGHTVYLERHICGRLFGEKFRHFNALGGWGELQNSVKTFGETHPFTKLVVDLTDIDPDVAYSSVPYEKGFALLFYLEQLLGGPEIFLGFLKAYVEKFSYKSITTDDWKDFLYSYFKDKVDVLNQVDWNAWLYSPGLPPIKPNYDMTLTNACIALSQRWITAKEDDLNSFNATDLKDLSSHQLNEFLAQTLQRAPLPLGHIKRMQEVYNFNAINNSEIRFRWLRLCIQSKWEDAIPLALKMATEQGRMKFTRPLFKDLAAFDKSHDQAVRTYQEHKASMHPVTAMLVGKDLKVD
Pathway:Lipid metabolism; leukotriene B4 biosynthesis.
Proteomics (Proteome ID):UP000005640
Proteomics (Chromosome): Chromosome 12
Active Site:ACT_SITE 297 297 Proton acceptor. {ECO:0000244|PDB:1H19, ECO:0000269|PubMed:11675384, ECO:0000305|PubMed:12207002}.; ACT_SITE 384 384 Proton donor. {ECO:0000244|PDB:1H19, ECO:0000269|PubMed:11675384, ECO:0000305|PubMed:12207002}.
Activity Regulation: Inhibited by bestatin. Subject to suicide inhibition by leukotriene A4, due to the formation of a covalent adduct at Tyr-379. {ECO:0000269|PubMed:7667299}.
Catalytic Activity: Reaction=H2O + leukotriene A4 = leukotriene B4; Xref=Rhea:RHEA:22324, ChEBI:CHEBI:15377, ChEBI:CHEBI:57461, ChEBI:CHEBI:57463; EC=3.3.2.6; Evidence={ECO:0000269|PubMed:11917124, ECO:0000269|PubMed:12207002, ECO:0000269|PubMed:15078870, ECO:0000269|PubMed:18804029, ECO:0000269|PubMed:1897988, ECO:0000269|PubMed:7667299};
Cofactor:Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000269|PubMed:12207002, ECO:0000269|PubMed:15078870, ECO:0000269|PubMed:18804029}; Note=Binds 1 zinc ion per subunit. {ECO:0000269|PubMed:12207002, ECO:0000269|PubMed:15078870, ECO:0000269|PubMed:18804029};
Function [CC]:Epoxide hydrolase that catalyzes the final step in the biosynthesis of the proinflammatory mediator leukotriene B4. Has also aminopeptidase activity. {ECO:0000269|PubMed:11917124, ECO:0000269|PubMed:12207002, ECO:0000269|PubMed:15078870, ECO:0000269|PubMed:18804029, ECO:0000269|PubMed:1897988, ECO:0000269|PubMed:1975494, ECO:0000269|PubMed:2244921}.
Metal Binding:METAL 296 296 Zinc; catalytic. {ECO:0000244|PDB:1GW6, ECO:0000244|PDB:1H19, ECO:0000244|PDB:1HS6, ECO:0000244|PDB:1SQM, ECO:0000244|PDB:2R59, ECO:0000244|PDB:2VJ8, ECO:0000244|PDB:3B7R, ECO:0000244|PDB:3B7S, ECO:0000244|PDB:3B7T, ECO:0000244|PDB:3B7U, ECO:0000244|PDB:3CHO, ECO:0000244|PDB:3CHP, ECO:0000244|PDB:3CHQ, ECO:0000244|PDB:3CHR, ECO:0000244|PDB:3CHS, ECO:0000244|PDB:3FH5, ECO:0000244|PDB:3FH7, ECO:0000244|PDB:3FH8, ECO:0000244|PDB:3FHE, ECO:0000244|PDB:3FTS, ECO:0000244|PDB:3FTU, ECO:0000244|PDB:3FTV, ECO:0000244|PDB:3FTW, ECO:0000244|PDB:3FTX, ECO:0000244|PDB:3FTY, ECO:0000244|PDB:3FTZ, ECO:0000244|PDB:3FU0, ECO:0000244|PDB:3FU3, ECO:0000244|PDB:3FU5, ECO:0000244|PDB:3FU6, ECO:0000244|PDB:3FUD, ECO:0000244|PDB:3FUE, ECO:0000244|PDB:3FUF, ECO:0000244|PDB:3FUH, ECO:0000244|PDB:3FUI, ECO:0000244|PDB:3FUJ, ECO:0000244|PDB:3FUK, ECO:0000244|PDB:3FUL, ECO:0000244|PDB:3FUM, ECO:0000244|PDB:3FUN, ECO:0000244|PDB:3U9W, ECO:0000244|PDB:4DPR, ECO:0000244|PDB:4L2L, ECO:0000244|PDB:4MKT, ECO:0000244|PDB:4MS6, ECO:0000244|PDB:5AEN, ECO:0000269|PubMed:11175901, ECO:0000269|PubMed:11675384, ECO:0000269|PubMed:11917124, ECO:0000269|PubMed:12207002, ECO:0000269|PubMed:15078870, ECO:0000269|PubMed:18804029, ECO:0000269|PubMed:19618939}.; METAL 300 300 Zinc; catalytic. {ECO:0000244|PDB:1GW6, ECO:0000244|PDB:1H19, ECO:0000244|PDB:1HS6, ECO:0000244|PDB:1SQM, ECO:0000244|PDB:2R59, ECO:0000244|PDB:2VJ8, ECO:0000244|PDB:3B7R, ECO:0000244|PDB:3B7S, ECO:0000244|PDB:3B7T, ECO:0000244|PDB:3B7U, ECO:0000244|PDB:3CHO, ECO:0000244|PDB:3CHP, ECO:0000244|PDB:3CHQ, ECO:0000244|PDB:3CHR, ECO:0000244|PDB:3CHS, ECO:0000244|PDB:3FH5, ECO:0000244|PDB:3FH7, ECO:0000244|PDB:3FH8, ECO:0000244|PDB:3FHE, ECO:0000244|PDB:3FTS, ECO:0000244|PDB:3FTU, ECO:0000244|PDB:3FTV, ECO:0000244|PDB:3FTW, ECO:0000244|PDB:3FTX, ECO:0000244|PDB:3FTY, ECO:0000244|PDB:3FTZ, ECO:0000244|PDB:3FU0, ECO:0000244|PDB:3FU3, ECO:0000244|PDB:3FU5, ECO:0000244|PDB:3FU6, ECO:0000244|PDB:3FUD, ECO:0000244|PDB:3FUE, ECO:0000244|PDB:3FUF, ECO:0000244|PDB:3FUH, ECO:0000244|PDB:3FUI, ECO:0000244|PDB:3FUJ, ECO:0000244|PDB:3FUK, ECO:0000244|PDB:3FUL, ECO:0000244|PDB:3FUM, ECO:0000244|PDB:3FUN, ECO:0000244|PDB:3U9W, ECO:0000244|PDB:4DPR, ECO:0000244|PDB:4L2L, ECO:0000244|PDB:4MKT, ECO:0000244|PDB:4MS6, ECO:0000244|PDB:5AEN, ECO:0000269|PubMed:11175901, ECO:0000269|PubMed:11675384, ECO:0000269|PubMed:11917124, ECO:0000269|PubMed:12207002, ECO:0000269|PubMed:15078870, ECO:0000269|PubMed:18804029, ECO:0000269|PubMed:19618939}.; METAL 319 319 Zinc; catalytic. {ECO:0000244|PDB:1GW6, ECO:0000244|PDB:1H19, ECO:0000244|PDB:1HS6, ECO:0000244|PDB:1SQM, ECO:0000244|PDB:2R59, ECO:0000244|PDB:2VJ8, ECO:0000244|PDB:3B7R, ECO:0000244|PDB:3B7S, ECO:0000244|PDB:3B7T, ECO:0000244|PDB:3B7U, ECO:0000244|PDB:3CHO, ECO:0000244|PDB:3CHP, ECO:0000244|PDB:3CHQ, ECO:0000244|PDB:3CHR, ECO:0000244|PDB:3CHS, ECO:0000244|PDB:3FH5, ECO:0000244|PDB:3FH7, ECO:0000244|PDB:3FH8, ECO:0000244|PDB:3FHE, ECO:0000244|PDB:3FTS, ECO:0000244|PDB:3FTU, ECO:0000244|PDB:3FTV, ECO:0000244|PDB:3FTW, ECO:0000244|PDB:3FTX, ECO:0000244|PDB:3FTY, ECO:0000244|PDB:3FTZ, ECO:0000244|PDB:3FU0, ECO:0000244|PDB:3FU3, ECO:0000244|PDB:3FU5, ECO:0000244|PDB:3FU6, ECO:0000244|PDB:3FUD, ECO:0000244|PDB:3FUE, ECO:0000244|PDB:3FUF, ECO:0000244|PDB:3FUH, ECO:0000244|PDB:3FUI, ECO:0000244|PDB:3FUJ, ECO:0000244|PDB:3FUK, ECO:0000244|PDB:3FUL, ECO:0000244|PDB:3FUM, ECO:0000244|PDB:3FUN, ECO:0000244|PDB:3U9W, ECO:0000244|PDB:4DPR, ECO:0000244|PDB:4L2L, ECO:0000244|PDB:4MKT, ECO:0000244|PDB:4MS6, ECO:0000244|PDB:5AEN, ECO:0000269|PubMed:11175901, ECO:0000269|PubMed:11675384, ECO:0000269|PubMed:11917124, ECO:0000269|PubMed:12207002, ECO:0000269|PubMed:15078870, ECO:0000269|PubMed:18804029, ECO:0000269|PubMed:19618939}.
Site:SITE 376 376 Essential for epoxide hydrolase activity, but not for aminopeptidase activity.; SITE 379 379 Covalently modified during suicide inhibition by leukotrienes.
Tissue Specificity:Isoform 1 and isoform 2 are expressed in monocytes, lymphocytes, neutrophils, reticulocytes, platelets and fibroblasts.
Chemical Profile | ChEBI:H2O [CHEBI:15377]; Zn(2+) [CHEBI:29105]; leukotriene B4 [CHEBI:57461]; leukotriene A4 [CHEBI:57463]
ChEBI (Catalytic Activity):H2O [CHEBI:15377]; leukotriene B4 [CHEBI:57461]; leukotriene A4 [CHEBI:57463]
ChEBI (Cofactor):Zn(2+) [CHEBI:29105]
Mutagenesis:MUTAGEN 135 135 Q->A,L: Srongly increased epoxide hydrolase activity. {ECO:0000269|PubMed:11917124}.; MUTAGEN 135 135 Q->A: Strongly reduced aminopeptidase activity. Strongly decreased affinity for leukotriene. Abolishes epoxide hydrolase activity. {ECO:0000269|PubMed:11917124}.; MUTAGEN 137 137 Q->A: No loss of activity. {ECO:0000269|PubMed:11675384}.; MUTAGEN 137 137 Q->L: Aminopeptidase activity strongly impaired, but keeps LTA4 activity. {ECO:0000269|PubMed:11675384}.; MUTAGEN 137 137 Q->N: Aminopeptidase activity almost absent, but keeps LTA4 activity. {ECO:0000269|PubMed:11675384}.; MUTAGEN 140 140 H->Q: Aminopeptidase activity almost absent, but keeps LTA4 activity. {ECO:0000269|PubMed:11917124}.; MUTAGEN 269 269 G->A: No loss of activity. {ECO:0000269|PubMed:1881903}.; MUTAGEN 270 270 G->A: No loss of activity. {ECO:0000269|PubMed:11675384}.; MUTAGEN 271 271 M->L: No loss of activity. {ECO:0000269|PubMed:11675384}.; MUTAGEN 272 272 E->A,D: Complete loss of activity. {ECO:0000269|PubMed:11675384}.; MUTAGEN 272 272 E->Q: Loss of LTA4 activity, and aminopeptidase activity strongly impaired. {ECO:0000269|PubMed:11675384}.; MUTAGEN 273 273 N->A: No loss of activity. {ECO:0000269|PubMed:11675384}.; MUTAGEN 296 296 H->Y: Complete loss of activity. {ECO:0000269|PubMed:1881903}.; MUTAGEN 297 297 E->A: Loss of both activities. {ECO:0000269|PubMed:1357660, ECO:0000269|PubMed:1516710, ECO:0000269|PubMed:18804029}.; MUTAGEN 297 297 E->K: Loss of both activities. {ECO:0000269|PubMed:1357660, ECO:0000269|PubMed:1516710, ECO:0000269|PubMed:18804029}.; MUTAGEN 297 297 E->Q: Loss of aminopeptidase activity, but keeps LTA4 activity. {ECO:0000269|PubMed:1357660, ECO:0000269|PubMed:1516710, ECO:0000269|PubMed:18804029}.; MUTAGEN 300 300 H->L: Complete loss of activity. {ECO:0000269|PubMed:1881903}.; MUTAGEN 319 319 E->A: Complete loss of activity. {ECO:0000269|PubMed:1881903}.; MUTAGEN 372 372 D->N: No loss of activity. {ECO:0000269|PubMed:11917124}.; MUTAGEN 374 374 D->N: No loss of activity. {ECO:0000269|PubMed:11917124}.; MUTAGEN 376 376 D->A: Strongly reduced hydrolysis of peptides starting with Arg. Small effect on hydrolysis of peptides starting with Ala. Strongly reduced epoxide hydrolase activity. {ECO:0000269|PubMed:11917124, ECO:0000269|PubMed:18804029}.; MUTAGEN 376 376 D->E: Strongly reduced aminopeptidase activity. Abolishes epoxide hydrolase activity. {ECO:0000269|PubMed:11917124, ECO:0000269|PubMed:18804029}.; MUTAGEN 376 376 D->N: Abolishes aminopeptidase and epoxide hydrolase activity. {ECO:0000269|PubMed:11917124, ECO:0000269|PubMed:18804029}.; MUTAGEN 385 385 E->Q: Reduced aminopeptidase activity. Minor effect on epoxide hydrolase activity. {ECO:0000269|PubMed:11917124}.; MUTAGEN 564 564 R->A,K,M: Abolishes epoxide hydrolase activity. Reduced aminopeptidase activity. {ECO:0000269|PubMed:15078870}.; MUTAGEN 566 566 K->A,M: Strongly reduced affinity for peptide substrates. Reduced epoxide hydrolase and aminopeptidase activity. {ECO:0000269|PubMed:15078870}.; MUTAGEN 566 566 K->R: No effect on epoxide hydrolase and aminopeptidase activity. {ECO:0000269|PubMed:15078870}.
Reagent Data
Name:LTA4H
Subcategory:Enzyme
Region:Met 1 - Asp 611
Molecular Weight:70.1
Source:HEK293
Species:Human
Tag:His
Format:Lyophilized
Purification System:Chromatography
Formulation:Sterile-filtered colorless solution
Formulation Concentration:1 mg/ml
Buffer Volume:Standard
Buffer Solution:PBS
pH:7.4-7.5
Toxicity
Endotoxin Level:< 1%
Aggregate Tested By:SDS-PAGE
Endotoxin Screened:< 0.1 ng/ug
Purity:> 98%
Determined: SDS-PAGE
Stain:Blue
Chromotography:Anion-exchange
Validated: RP-HPLC
Sample Handling
Storage:-20°C
Stability:This bioreagent is stable at 4°C (short-term) and -70°C(long-term). After reconstitution, sample may be stored at 4°C for 2-7 days and below -18°C for future use.
Preparation:Reconstitute in sterile distilled H2O to no less than 100 ug/ml; dilute reconstituted stock further in other aqueous solutions if needed. Please review COA for lot-specific instructions. Final measurements should be determined by the end-user for optimal performance.