HAO-1
Human Recombinant HAO1 - His Tagged
Reference ID:KB-3924
Western Blot
ELISA
FACS
Protein:Protein
Flow Cytometry
Gene of Interest
Gene Synonyms:HAO1;GOX1;HAOX1
Protein Names:Hydroxyacid oxidase 1 (HAOX1) (EC 1.1.3.15) (Glycolate oxidase) (GOX)
Accession Data
Organism:Homo sapiens (Human)
Mass (kDa):409.24
Length (aa):370
Sequence:MLPRLICINDYEQHAKSVLPKSIYDYYRSGANDEETLADNIAAFSRWKLYPRMLRNVAETDLSTSVLGQRVSMPICVGATAMQRMAHVDGELATVRACQSLGTGMMLSSWATSSIEEVAEAGPEALRWLQLYIYKDREVTKKLVRQAEKMGYKAIFVTVDTPYLGNRLDDVRNRFKLPPQLRMKNFETSTLSFSPEENFGDDSGLAAYVAKAIDPSISWEDIKWLRRLTSLPIVAKGILRGDDAREAVKHGLNGILVSNHGARQLDGVPATIDVLPEIVEAVEGKVEVFLDGGVRKGTDVLKALALGAKAVFVGRPIVWGLAFQGEKGVQDVLEILKEEFRLAMALSGCQNVKVIDKTLVRKNPLAVSKI
Pathway:Organic acid metabolism; glycolate degradation; 3-phospho-D-glycerate from glycolate: step 1/4.
Proteomics (Proteome ID):UP000005640
Proteomics (Chromosome): Chromosome 20
Active Site:ACT_SITE 260 260 Proton acceptor.
Binding Site:BINDING 26 26 Substrate.; BINDING 108 108 FMN. {ECO:0000255|PROSITE-ProRule:PRU00683, ECO:0000269|PubMed:18215067, ECO:0000269|PubMed:20054120, ECO:0000269|Ref.10}.; BINDING 130 130 FMN. {ECO:0000255|PROSITE-ProRule:PRU00683, ECO:0000269|PubMed:18215067, ECO:0000269|PubMed:20054120, ECO:0000269|Ref.10}.; BINDING 132 132 FMN. {ECO:0000255|PROSITE-ProRule:PRU00683, ECO:0000269|PubMed:18215067, ECO:0000269|PubMed:20054120, ECO:0000269|Ref.10}.; BINDING 132 132 Substrate.; BINDING 158 158 FMN. {ECO:0000255|PROSITE-ProRule:PRU00683, ECO:0000269|PubMed:18215067, ECO:0000269|PubMed:20054120, ECO:0000269|Ref.10}.; BINDING 167 167 Substrate.; BINDING 236 236 FMN. {ECO:0000255|PROSITE-ProRule:PRU00683, ECO:0000269|PubMed:18215067, ECO:0000269|PubMed:20054120, ECO:0000269|Ref.10}.; BINDING 258 258 FMN. {ECO:0000255|PROSITE-ProRule:PRU00683, ECO:0000269|PubMed:18215067, ECO:0000269|PubMed:20054120, ECO:0000269|Ref.10}.; BINDING 263 263 Substrate.; BINDING 315 315 FMN. {ECO:0000255|PROSITE-ProRule:PRU00683, ECO:0000269|PubMed:18215067, ECO:0000269|PubMed:20054120, ECO:0000269|Ref.10}.
Catalytic Activity: Reaction=a (2S)-2-hydroxycarboxylate + O2 = a 2-oxocarboxylate + H2O2; Xref=Rhea:RHEA:16789, ChEBI:CHEBI:15379, ChEBI:CHEBI:16240, ChEBI:CHEBI:35179, ChEBI:CHEBI:58123; EC=1.1.3.15; Evidence={ECO:0000269|PubMed:18215067};
Cofactor:Name=FMN; Xref=ChEBI:CHEBI:58210; Evidence={ECO:0000255|PROSITE-ProRule:PRU00683, ECO:0000269|PubMed:18215067};
Function [CC]:Has 2-hydroxyacid oxidase activity. Most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids, with high activity towards 2-hydroxy palmitate and 2-hydroxy octanoate. {ECO:0000269|PubMed:18215067}.
Kinetics:BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=141 uM for glycolate {ECO:0000269|PubMed:18215067}; KM=40 uM for 2-hydroxy octanoate {ECO:0000269|PubMed:18215067}; KM=2200 uM for glyoxylate {ECO:0000269|PubMed:18215067};
Nucleotide Binding:NP_BIND 291 315 FMN. {ECO:0000255|PROSITE-ProRule:PRU00683}.; NP_BIND 291 295 FMN binding. {ECO:0000255|PROSITE-ProRule:PRU00683, ECO:0000269|PubMed:18215067, ECO:0000269|PubMed:20054120, ECO:0000269|Ref.10}.
Tissue Specificity:Liver.
Chemical Profile | ChEBI:H2O2 [CHEBI:16240]; FMN [CHEBI:58210]; O2 [CHEBI:15379]; a (2S)-2-hydroxycarboxylate [CHEBI:58123]; a 2-oxocarboxylate [CHEBI:35179]
ChEBI (Catalytic Activity):H2O2 [CHEBI:16240]; O2 [CHEBI:15379]; a (2S)-2-hydroxycarboxylate [CHEBI:58123]; a 2-oxocarboxylate [CHEBI:35179]
ChEBI (Cofactor):FMN [CHEBI:58210]
Reagent Data
Name:HAO-1
Subcategory:Enzyme
Region:Leu 2 - Ile 370
Molecular Weight:41.7
Source:HEK293
Species:Human
Tag:His
Format:Lyophilized
Purification System:Chromatography
Formulation:Sterile-filtered colorless solution
Formulation Concentration:1 mg/ml
Buffer Volume:Standard
Buffer Solution:PBS
pH:7.4-7.5
Toxicity
Endotoxin Level:< 1%
Aggregate Tested By:SDS-PAGE
Endotoxin Screened:< 0.1 ng/ug
Purity:> 98%
Determined: SDS-PAGE
Stain:Blue
Chromotography:Anion-exchange
Validated: RP-HPLC
Sample Handling
Storage:-20°C
Stability:This bioreagent is stable at 4°C (short-term) and -70°C(long-term). After reconstitution, sample may be stored at 4°C for 2-7 days and below -18°C for future use.
Preparation:Reconstitute in sterile distilled H2O to no less than 100 ug/ml; dilute reconstituted stock further in other aqueous solutions if needed. Please review COA for lot-specific instructions. Final measurements should be determined by the end-user for optimal performance.