Furin
Human Recombinant FURIN - His Tagged
Reference ID:KB-3923
Western Blot
ELISA
FACS
Protein:Protein
Flow Cytometry
Gene of Interest
Gene Synonyms:FURIN;FUR;PACE;PCSK3
Protein Names:Furin (EC 3.4.21.75) (Dibasic-processing enzyme) (Paired basic amino acid residue-cleaving enzyme) (PACE)
Accession Data
Organism:Homo sapiens (Human)
Mass (kDa):866.78
Length (aa):794
Sequence:MELRPWLLWVVAATGTLVLLAADAQGQKVFTNTWAVRIPGGPAVANSVARKHGFLNLGQIFGDYYHFWHRGVTKRSLSPHRPRHSRLQREPQVQWLEQQVAKRRTKRDVYQEPTDPKFPQQWYLSGVTQRDLNVKAAWAQGYTGHGIVVSILDDGIEKNHPDLAGNYDPGASFDVNDQDPDPQPRYTQMNDNRHGTRCAGEVAAVANNGVCGVGVAYNARIGGVRMLDGEVTDAVEARSLGLNPNHIHIYSASWGPEDDGKTVDGPARLAEEAFFRGVSQGRGGLGSIFVWASGNGGREHDSCNCDGYTNSIYTLSISSATQFGNVPWYSEACSSTLATTYSSGNQNEKQIVTTDLRQKCTESHTGTSASAPLAAGIIALTLEANKNLTWRDMQHLVVQTSKPAHLNANDWATNGVGRKVSHSYGYGLLDAGAMVALAQNWTTVAPQRKCIIDILTEPKDIGKRLEVRKTVTACLGEPNHITRLEHAQARLTLSYNRRGDLAIHLVSPMGTRSTLLAARPHDYSADGFNDWAFMTTHSWDEDPSGEWVLEIENTSEANNYGTLTKFTLVLYGTAPEGLPVPPESSGCKTLTSSQACVVCEEGFSLHQKSCVQHCPPGFAPQVLDTHYSTENDVETIRASVCAPCHASCATCQGPALTDCLSCPSHASLDPVEQTCSRQSQSSRESPPQQQPPRLPPEVEAGQRLRAGLLPSHLPEVVAGLSCAFIVLVFVTVFLVLQLRSGFSFRGVKVYTMDRGLISYKGLPPEAWQEECPSDSEEDEGRGERTAFIKDQSAL
Proteomics (Proteome ID):UP000005640
Proteomics (Chromosome): Chromosome 15
Active Site:ACT_SITE 153 153 Charge relay system. {ECO:0000255|PROSITE-ProRule:PRU10080}.; ACT_SITE 194 194 Charge relay system. {ECO:0000255|PROSITE-ProRule:PRU10081}.; ACT_SITE 368 368 Charge relay system. {ECO:0000255|PROSITE-ProRule:PRU10082}.
Activity Regulation: Inhibited by the not secondly cleaved propeptide (PubMed:9130696, PubMed:11799113). Inhibited by m-guanidinomethyl-phenylacetyl-Arg-Val-Arg-(amidomethyl)-benzamidine (m-guanidinomethyl-Phac-RVR-Amb) and 4-guanidinomethyl-phenylacteyl-Arg-Tle-Arg-4-amidinobenzylamide (MI-1148) (PubMed:24666235, PubMed:25974265). {ECO:0000269|PubMed:11799113, ECO:0000269|PubMed:24666235, ECO:0000269|PubMed:25974265, ECO:0000269|PubMed:9130696}.
Binding Site:BINDING 154 154 Substrate. {ECO:0000244|PDB:4OMC, ECO:0000244|PDB:4OMD, ECO:0000244|PDB:4RYD, ECO:0000305|PubMed:24666235, ECO:0000305|PubMed:25974265}.; BINDING 236 236 Substrate. {ECO:0000244|PDB:4OMC, ECO:0000244|PDB:4OMD, ECO:0000244|PDB:4RYD, ECO:0000305|PubMed:24666235, ECO:0000305|PubMed:25974265}.; BINDING 264 264 Substrate. {ECO:0000244|PDB:4OMC, ECO:0000244|PDB:4OMD, ECO:0000244|PDB:4RYD, ECO:0000305|PubMed:24666235, ECO:0000305|PubMed:25974265}.; BINDING 306 306 Substrate. {ECO:0000244|PDB:4OMC, ECO:0000244|PDB:4OMD, ECO:0000244|PDB:4RYD, ECO:0000305|PubMed:24666235, ECO:0000305|PubMed:25974265}.; BINDING 308 308 Substrate. {ECO:0000244|PDB:4OMC, ECO:0000244|PDB:4OMD, ECO:0000244|PDB:4RYD, ECO:0000305|PubMed:24666235, ECO:0000305|PubMed:25974265}.; BINDING 368 368 Substrate. {ECO:0000244|PDB:4OMC, ECO:0000244|PDB:4OMD, ECO:0000244|PDB:4RYD, ECO:0000305|PubMed:24666235, ECO:0000305|PubMed:25974265}.
Catalytic Activity: Reaction=Release of mature proteins from their proproteins by cleavage of -Arg-Xaa-Yaa-Arg-|-Zaa- bonds, where Xaa can be any amino acid and Yaa is Arg or Lys. Releases albumin, complement component C3 and von Willebrand factor from their respective precursors.; EC=3.4.21.75; Evidence={ECO:0000269|PubMed:11799113, ECO:0000269|PubMed:1629222, ECO:0000269|PubMed:1713771, ECO:0000269|PubMed:2251280, ECO:0000269|PubMed:24666235, ECO:0000269|PubMed:25974265, ECO:0000269|PubMed:7592877, ECO:0000269|PubMed:7690548, ECO:0000269|PubMed:7737999, ECO:0000269|PubMed:9130696};
Cofactor:Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence={ECO:0000269|PubMed:24666235, ECO:0000269|PubMed:25974265, ECO:0000269|PubMed:9130696}; Note=Binds 3 calcium ions per subunit. {ECO:0000269|PubMed:24666235, ECO:0000269|PubMed:25974265, ECO:0000269|PubMed:9130696};
Function [CC]:Ubiquitous endoprotease within constitutive secretory pathways capable of cleavage at the RX(K/R)R consensus motif (PubMed:11799113, PubMed:1629222, PubMed:1713771, PubMed:2251280, PubMed:24666235, PubMed:25974265, PubMed:7592877, PubMed:7690548, PubMed:9130696). Mediates processing of TGFB1, an essential step in TGF-beta-1 activation (PubMed:7737999). {ECO:0000269|PubMed:11799113, ECO:0000269|PubMed:1629222, ECO:0000269|PubMed:1713771, ECO:0000269|PubMed:2251280, ECO:0000269|PubMed:24666235, ECO:0000269|PubMed:25974265, ECO:0000269|PubMed:7592877, ECO:0000269|PubMed:7690548, ECO:0000269|PubMed:7737999, ECO:0000269|PubMed:9130696}.; (Microbial infection) Probably cleaves and activates anthrax and diphtheria toxins.; (Microbial infection) Required for H7N1 and H5N1 influenza virus infection probably by cleaving hemagglutinin. {ECO:0000269|PubMed:25974265}.; (Microbial infection) Able to cleave S.pneumoniae serine-rich repeat protein PsrP. {ECO:0000269|PubMed:27582320}.
Metal Binding:METAL 115 115 Calcium 1. {ECO:0000244|PDB:4OMC, ECO:0000244|PDB:4OMD, ECO:0000244|PDB:4RYD, ECO:0000269|PubMed:24666235, ECO:0000269|PubMed:25974265}.; METAL 162 162 Calcium 1. {ECO:0000244|PDB:4OMC, ECO:0000244|PDB:4OMD, ECO:0000244|PDB:4RYD, ECO:0000269|PubMed:24666235, ECO:0000269|PubMed:25974265}.; METAL 174 174 Calcium 2. {ECO:0000244|PDB:4OMC, ECO:0000244|PDB:4OMD, ECO:0000244|PDB:4RYD, ECO:0000269|PubMed:24666235, ECO:0000269|PubMed:25974265}.; METAL 179 179 Calcium 2. {ECO:0000244|PDB:4OMC, ECO:0000244|PDB:4OMD, ECO:0000244|PDB:4RYD, ECO:0000269|PubMed:24666235, ECO:0000269|PubMed:25974265}.; METAL 181 181 Calcium 2; via carbonyl oxygen. {ECO:0000244|PDB:4OMC, ECO:0000244|PDB:4OMD, ECO:0000244|PDB:4RYD, ECO:0000269|PubMed:24666235, ECO:0000269|PubMed:25974265}.; METAL 205 205 Calcium 1; via carbonyl oxygen. {ECO:0000244|PDB:4OMC, ECO:0000244|PDB:4OMD, ECO:0000244|PDB:4RYD, ECO:0000269|PubMed:24666235, ECO:0000269|PubMed:25974265}.; METAL 208 208 Calcium 1. {ECO:0000244|PDB:4OMC, ECO:0000244|PDB:4OMD, ECO:0000244|PDB:4RYD, ECO:0000269|PubMed:24666235, ECO:0000269|PubMed:25974265}.; METAL 210 210 Calcium 1; via carbonyl oxygen. {ECO:0000244|PDB:4OMC, ECO:0000244|PDB:4OMD, ECO:0000244|PDB:4RYD, ECO:0000269|PubMed:24666235, ECO:0000269|PubMed:25974265}.; METAL 212 212 Calcium 1; via carbonyl oxygen. {ECO:0000244|PDB:4OMC, ECO:0000244|PDB:4OMD, ECO:0000244|PDB:4RYD, ECO:0000269|PubMed:24666235, ECO:0000269|PubMed:25974265}.; METAL 258 258 Calcium 3. {ECO:0000244|PDB:4OMC, ECO:0000244|PDB:4OMD, ECO:0000244|PDB:4RYD, ECO:0000269|PubMed:24666235, ECO:0000269|PubMed:25974265}.; METAL 301 301 Calcium 3. {ECO:0000244|PDB:4OMC, ECO:0000244|PDB:4OMD, ECO:0000244|PDB:4RYD, ECO:0000269|PubMed:24666235, ECO:0000269|PubMed:25974265}.; METAL 331 331 Calcium 3. {ECO:0000244|PDB:4OMC, ECO:0000244|PDB:4OMD, ECO:0000244|PDB:4RYD, ECO:0000269|PubMed:24666235, ECO:0000269|PubMed:25974265}.
Site:SITE 75 76 Cleavage, second; by autolysis. {ECO:0000269|PubMed:9130696}.; SITE 107 108 Cleavage, first; by autolysis. {ECO:0000269|PubMed:1629222, ECO:0000269|PubMed:9130696}.
Tissue Specificity:Seems to be expressed ubiquitously. {ECO:0000269|PubMed:1713771}.
Chemical Profile | ChEBI:Ca(2+) [CHEBI:29108]
ChEBI (Cofactor):Ca(2+) [CHEBI:29108]
Mutagenesis:MUTAGEN 72 72 V->R: Loss of catalytic activity and propeptide second cleavage and removal. Abnormal accumulation in the early secretory pathway. {ECO:0000269|PubMed:11799113}.; MUTAGEN 75 75 R->A: Loss of catalytic activity and, propeptide second cleavage and removal. Normal trafficking to the Golgi. {ECO:0000269|PubMed:11799113}.; MUTAGEN 153 153 D->N: Loss of catalytic activity and propeptide first cleavage. Abnormal accumulation in the early secretory pathway. {ECO:0000269|PubMed:11799113, ECO:0000269|PubMed:9130696}.; MUTAGEN 773 775 SDS->DDD: Phosphomimetic mutant. Localization in early endosome is increased. {ECO:0000269|PubMed:8846780}.; MUTAGEN 773 773 S->A: Slight reduction in phosphorylation. Loss of phosphorylation and abnormal accumulation in the early secretory pathway; when associated with A-775. {ECO:0000269|PubMed:8846780}.; MUTAGEN 775 775 S->A: Slight reduction in phosphorylation. Loss of phosphorylation and abnormal accumulation in the early secretory pathway; when associated with A-773. {ECO:0000269|PubMed:8846780}.
Reagent Data
Name:Furin
Subcategory:Enzyme
Region:Gln 25 - Gly 577
Molecular Weight:61.5
Source:HEK293
Species:Human
Tag:His
Format:Lyophilized
Purification System:Chromatography
Formulation:Sterile-filtered colorless solution
Formulation Concentration:1 mg/ml
Buffer Volume:Standard
Buffer Solution:PBS
pH:7.4-7.5
Toxicity
Endotoxin Level:< 1%
Aggregate Tested By:SDS-PAGE
Endotoxin Screened:< 0.1 ng/ug
Purity:> 98%
Determined: SDS-PAGE
Stain:Blue
Chromotography:Anion-exchange
Validated: RP-HPLC
Sample Handling
Storage:-20°C
Stability:This bioreagent is stable at 4°C (short-term) and -70°C(long-term). After reconstitution, sample may be stored at 4°C for 2-7 days and below -18°C for future use.
Preparation:Reconstitute in sterile distilled H2O to no less than 100 ug/ml; dilute reconstituted stock further in other aqueous solutions if needed. Please review COA for lot-specific instructions. Final measurements should be determined by the end-user for optimal performance.