DPPIV
Human Recombinant DPP4
Reference ID:KB-3921
Western Blot
ELISA
FACS
Protein:Protein
Flow Cytometry
Gene of Interest
Gene Synonyms:DPP4;ADCP2;CD26
Protein Names:Dipeptidyl peptidase 4 (EC 3.4.14.5) (ADABP) (Adenosine deaminase complexing protein 2) (ADCP-2) (Dipeptidyl peptidase IV) (DPP IV) (T-cell activation antigen CD26) (TP103) (CD antigen CD26) [Cleaved into: Dipeptidyl peptidase 4 membrane form (Dipeptidyl peptidase IV membrane form); Dipeptidyl peptidase 4 soluble form (Dipeptidyl peptidase IV soluble form)]
Accession Data
Organism:Homo sapiens (Human)
Mass (kDa):882.79
Length (aa):766
Sequence:MKTPWKVLLGLLGAAALVTIITVPVVLLNKGTDDATADSRKTYTLTDYLKNTYRLKLYSLRWISDHEYLYKQENNILVFNAEYGNSSVFLENSTFDEFGHSINDYSISPDGQFILLEYNYVKQWRHSYTASYDIYDLNKRQLITEERIPNNTQWVTWSPVGHKLAYVWNNDIYVKIEPNLPSYRITWTGKEDIIYNGITDWVYEEEVFSAYSALWWSPNGTFLAYAQFNDTEVPLIEYSFYSDESLQYPKTVRVPYPKAGAVNPTVKFFVVNTDSLSSVTNATSIQITAPASMLIGDHYLCDVTWATQERISLQWLRRIQNYSVMDICDYDESSGRWNCLVARQHIEMSTTGWVGRFRPSEPHFTLDGNSFYKIISNEEGYRHICYFQIDKKDCTFITKGTWEVIGIEALTSDYLYYISNEYKGMPGGRNLYKIQLSDYTKVTCLSCELNPERCQYYSVSFSKEAKYYQLRCSGPGLPLYTLHSSVNDKGLRVLEDNSALDKMLQNVQMPSKKLDFIILNETKFWYQMILPPHFDKSKKYPLLLDVYAGPCSQKADTVFRLNWATYLASTENIIVASFDGRGSGYQGDKIMHAINRRLGTFEVEDQIEAARQFSKMGFVDNKRIAIWGWSYGGYVTSMVLGSGSGVFKCGIAVAPVSRWEYYDSVYTERYMGLPTPEDNLDHYRNSTVMSRAENFKQVEYLLIHGTADDNVHFQQSAQISKALVDVGVDFQAMWYTDEDHGIASSTAHQHIYTHMSHFIKQCFSLP
Proteomics (Proteome ID):UP000005640
Proteomics (Chromosome): Chromosome 2
Active Site:ACT_SITE 630 630 Charge relay system. {ECO:0000255|PROSITE-ProRule:PRU10084}.; ACT_SITE 708 708 Charge relay system. {ECO:0000255|PROSITE-ProRule:PRU10084}.; ACT_SITE 740 740 Charge relay system. {ECO:0000255|PROSITE-ProRule:PRU10084}.
Activity Regulation: Inhibited by GPC3 and diprotin A. {ECO:0000269|PubMed:17549790, ECO:0000269|PubMed:18708048}.
Catalytic Activity: Reaction=Release of an N-terminal dipeptide, Xaa-Yaa-|-Zaa-, from a polypeptide, preferentially when Yaa is Pro, provided Zaa is neither Pro nor hydroxyproline.; EC=3.4.14.5; Evidence={ECO:0000255|PROSITE-ProRule:PRU10084, ECO:0000269|PubMed:10593948};
Function [CC]:Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC. Its binding to CAV1 and CARD11 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion. In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May be involved in the promotion of lymphatic endothelial cells adhesion, migration and tube formation. When overexpressed, enhanced cell proliferation, a process inhibited by GPC3. Acts also as a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones. Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline. {ECO:0000269|PubMed:10570924, ECO:0000269|PubMed:10593948, ECO:0000269|PubMed:10900005, ECO:0000269|PubMed:10951221, ECO:0000269|PubMed:11772392, ECO:0000269|PubMed:14691230, ECO:0000269|PubMed:16651416, ECO:0000269|PubMed:17287217, ECO:0000269|PubMed:17549790, ECO:0000269|PubMed:18708048}.
Induction:Up-regulated by IL12/interleukin-12 on activated T-cells. IL12-activated cells expressed enhanced levels of DPP4 but not mRNAs. Down-regulated by TNF. Up-regulated in migratory endothelial cells and in the invasive endothelial cells in tumors. Induced by hypoxia (PubMed:16670267). {ECO:0000269|PubMed:10880264, ECO:0000269|PubMed:12676959, ECO:0000269|PubMed:16651416, ECO:0000269|PubMed:16670267, ECO:0000269|PubMed:9413751}.
Tissue Specificity:Expressed specifically in lymphatic vessels but not in blood vessels in the skin, small intestine, esophagus, ovary, breast and prostate glands. Not detected in lymphatic vessels in the lung, kidney, uterus, liver and stomach (at protein level). Expressed in the poorly differentiated crypt cells of the small intestine as well as in the mature villous cells. Expressed at very low levels in the colon. {ECO:0000269|PubMed:1677636, ECO:0000269|PubMed:18708048}.
Mutagenesis:MUTAGEN 85 85 N->A: Does not inhibit dipeptidyl peptidase activity, interaction with ADA and homodimer formation. {ECO:0000269|PubMed:14691230}.; MUTAGEN 92 92 N->A: Does not inhibit dipeptidyl peptidase activity, interaction with ADA and homodimer formation. {ECO:0000269|PubMed:14691230}.; MUTAGEN 150 150 N->A: Does not inhibit dipeptidyl peptidase activity, interaction with ADA and homodimer formation. {ECO:0000269|PubMed:14691230}.; MUTAGEN 205 205 E->K: Inhibits dipeptidyl peptidase activity. {ECO:0000269|PubMed:10570924}.; MUTAGEN 206 206 E->L: Inhibits dipeptidyl peptidase activity. {ECO:0000269|PubMed:10570924}.; MUTAGEN 219 219 N->A: Does not inhibit dipeptidyl peptidase activity, interaction with ADA and homodimer formation. {ECO:0000269|PubMed:14691230}.; MUTAGEN 229 229 N->A: Does not inhibit dipeptidyl peptidase activity, interaction with ADA and homodimer formation. {ECO:0000269|PubMed:14691230}.; MUTAGEN 281 281 N->A: Does not inhibit dipeptidyl peptidase activity, interaction with ADA and homodimer formation. {ECO:0000269|PubMed:14691230}.; MUTAGEN 321 321 N->A: Does not inhibit dipeptidyl peptidase activity, interaction with ADA and homodimer formation. {ECO:0000269|PubMed:14691230}.; MUTAGEN 520 520 N->A: Does not inhibit dipeptidyl peptidase activity, interaction with ADA and homodimer formation. {ECO:0000269|PubMed:14691230}.; MUTAGEN 685 685 N->A: Does not inhibit dipeptidyl peptidase activity, interaction with ADA and homodimer formation. {ECO:0000269|PubMed:14691230}.; MUTAGEN 750 750 H->A: Inhibits weakly homodimerization and dipeptidyl peptidase activity. {ECO:0000269|PubMed:15448155, ECO:0000269|PubMed:17287217}.; MUTAGEN 750 750 H->E: Inhibits strongly homodimerization, dipeptidyl peptidase activity, interaction with CARD11 and T-cell costimulation activity. {ECO:0000269|PubMed:15448155, ECO:0000269|PubMed:17287217}.
Miscellaneous [CC]:Level of plasma concentrations of the soluble form (SDPP) can be managed as a colon carcinoma diagnostic and prognostic marker.
Reagent Data
Name:DPPIV
Subcategory:Enzyme
Region:Asn 29 - Pro 766
Molecular Weight:85.4
Source:HEK293
Species:Human
Tag:
Format:Lyophilized
Purification System:Chromatography
Formulation:Sterile-filtered colorless solution
Formulation Concentration:1 mg/ml
Buffer Volume:Standard
Buffer Solution:PBS
pH:7.4-7.5
Toxicity
Endotoxin Level:< 1%
Aggregate Tested By:SDS-PAGE
Endotoxin Screened:< 0.1 ng/ug
Purity:> 98%
Determined: SDS-PAGE
Stain:Blue
Chromotography:Anion-exchange
Validated: RP-HPLC
Sample Handling
Storage:-20°C
Stability:This bioreagent is stable at 4°C (short-term) and -70°C(long-term). After reconstitution, sample may be stored at 4°C for 2-7 days and below -18°C for future use.
Preparation:Reconstitute in sterile distilled H2O to no less than 100 ug/ml; dilute reconstituted stock further in other aqueous solutions if needed. Please review COA for lot-specific instructions. Final measurements should be determined by the end-user for optimal performance.