Carboxypeptidase E
Mouse Recombinant Cpe - His Tagged
Reference ID:KB-3910
Western Blot
ELISA
FACS
Protein:Protein
Flow Cytometry
Gene of Interest
Gene Synonyms:Cpe
Protein Names:Carboxypeptidase E (CPE) (EC 3.4.17.10) (Carboxypeptidase H) (CPH) (Enkephalin convertase) (Prohormone-processing carboxypeptidase)
Accession Data
Organism:Mus musculus (Mouse)
Mass (kDa):532.56
Length (aa):476
Sequence:MAGRGGRVLLALCAALVAGGWLLTAEAQEPGAPAAGMRRRRRLQQEDGISFEYHRYPELREALVSVWLQCTAISRIYTVGRSFEGRELLVIELSDNPGVHEPGEPEFKYIGNMHGNEAVGRELLIFLAQYLCNEYQKGNETIVNLIHSTRIHIMPSLNPDGFEKAASQPGELKDWFVGRSNAQGIDLNRNFPDLDRIVYVNEKEGGPNNHLLKNLKKIVDQNSKLAPETKAVIHWIMDIPFVLSANLHGGDLVANYPYDETRSGTAHEYSSCPDDAIFQSLARAYSSFNPVMSDPNRPPCRKNDDDSSFVDGTTNGGAWYSVPGGMQDFNYLSSNCFEITVELSCEKFPPEETLKSYWEDNKNSLISYLEQIHRGVKGFVRDLQGNPIANATISVDGIDHDVTSAKDGDYWRLLAPGNYKLTASAPGYLAITKKVAVPFSPAVGVDFELESFSERKEEEKEELMEWWKMMSETLNF
Proteomics (Proteome ID):UP000000589
Proteomics (Chromosome): Chromosome 8
Active Site:ACT_SITE 342 342 Proton donor/acceptor. {ECO:0000250|UniProtKB:P14384}.
Catalytic Activity: Reaction=Release of C-terminal arginine or lysine residues from polypeptides.; EC=3.4.17.10;
Cofactor:Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000250|UniProtKB:P00730}; Note=Binds 1 zinc ion per subunit. {ECO:0000250|UniProtKB:P00730};
Function [CC]:Sorting receptor that directs prohormones to the regulated secretory pathway. Acts also as a prohormone processing enzyme in neuro/endocrine cells, removing dibasic residues from the C-terminal end of peptide hormone precursors after initial endoprotease cleavage. {ECO:0000269|PubMed:10896946, ECO:0000269|PubMed:16219686}.
Metal Binding:METAL 114 114 Zinc; catalytic. {ECO:0000250|UniProtKB:P00730}.; METAL 117 117 Zinc; catalytic. {ECO:0000250|UniProtKB:P00730}.; METAL 248 248 Zinc; catalytic. {ECO:0000250|UniProtKB:P00730}.
Chemical Profile | ChEBI:Zn(2+) [CHEBI:29105]
ChEBI (Cofactor):Zn(2+) [CHEBI:29105]
Disease:Note=Defects in Cpe are the cause of the fat phenotype. Mice homozygous for the fat mutation develop obesity and hyperglycemia that can be suppressed by treatment with exogenous insulin. {ECO:0000269|PubMed:7663508}.
Reagent Data
Name:Carboxypeptidase E
Subcategory:Enzyme
Region:Gln 28 - Phe 476
Molecular Weight:52
Source:HEK293
Species:Mouse
Tag:His
Format:Lyophilized
Purification System:Chromatography
Formulation:Sterile-filtered colorless solution
Formulation Concentration:1 mg/ml
Buffer Volume:Standard
Buffer Solution:PBS
pH:7.4-7.5
Toxicity
Endotoxin Level:< 1%
Aggregate Tested By:SDS-PAGE
Endotoxin Screened:< 0.1 ng/ug
Purity:> 98%
Determined: SDS-PAGE
Stain:Blue
Chromotography:Anion-exchange
Validated: RP-HPLC
Sample Handling
Storage:-20°C
Stability:This bioreagent is stable at 4°C (short-term) and -70°C(long-term). After reconstitution, sample may be stored at 4°C for 2-7 days and below -18°C for future use.
Preparation:Reconstitute in sterile distilled H2O to no less than 100 ug/ml; dilute reconstituted stock further in other aqueous solutions if needed. Please review COA for lot-specific instructions. Final measurements should be determined by the end-user for optimal performance.