Carboxypeptidase A4
Human Recombinant CPA4 - His Tagged
Reference ID:KB-3908
Western Blot
ELISA
FACS
Protein:Protein
Flow Cytometry
Gene of Interest
Gene Synonyms:CPA4;CPA3;UNQ694/PRO1339
Protein Names:Carboxypeptidase A4 (EC 3.4.17.-) (Carboxypeptidase A3)
Accession Data
Organism:Homo sapiens (Human)
Mass (kDa):473.51
Length (aa):421
Sequence:MRWILFIGALIGSSICGQEKFFGDQVLRINVRNGDEISKLSQLVNSNNLKLNFWKSPSSFNRPVDVLVPSVSLQAFKSFLRSQGLEYAVTIEDLQALLDNEDDEMQHNEGQERSSNNFNYGAYHSLEAIYHEMDNIAADFPDLARRVKIGHSFENRPMYVLKFSTGKGVRRPAVWLNAGIHSREWISQATAIWTARKIVSDYQRDPAITSILEKMDIFLLPVANPDGYVYTQTQNRLWRKTRSRNPGSSCIGADPNRNWNASFAGKGASDNPCSEVYHGPHANSEVEVKSVVDFIQKHGNFKGFIDLHSYSQLLMYPYGYSVKKAPDAEELDKVARLAAKALASVSGTEYQVGPTCTTVYPASGSSIDWAYDNGIKFAFTFELRDTGTYGFLLPANQIIPTAEETWLGLKTIMEHVRDNLY
Proteomics (Proteome ID):UP000005640
Proteomics (Chromosome): Chromosome 7
Active Site:ACT_SITE 382 382 Proton donor/acceptor. {ECO:0000250|UniProtKB:P00732}.
Activity Regulation: Inhibited by interaction with the metallocarboxypeptidase inhibitor (MCPI) from N.versicolor that binds to the catalytic zinc ion. {ECO:0000269|PubMed:22294694}.
Binding Site:BINDING 239 239 Substrate. {ECO:0000250|UniProtKB:P00730}.; BINDING 360 360 Substrate. {ECO:0000250|UniProtKB:P00730}.
Cofactor:Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000269|PubMed:22294694}; Note=Binds 1 zinc ion per subunit. {ECO:0000269|PubMed:22294694};
Function [CC]:Metalloprotease that could be involved in the histone hyperacetylation pathway (PubMed:10383164). Releases a C-terminal amino acid, with preference for -Phe, -Leu, -Ile, -Met, -Tyr and -Val (PubMed:20385563). {ECO:0000269|PubMed:10383164, ECO:0000269|PubMed:20385563}.
Kinetics:BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=55.6 uM for 3-(2-furyl)acryloyl-Phe-Phe {ECO:0000269|PubMed:20385563}; KM=19.4 uM for 3-(2-furyl)acryloyl-Phe-Leu {ECO:0000269|PubMed:20385563}; KM=23.3 uM for 3-(2-furyl)acryloyl-Phe-Ile {ECO:0000269|PubMed:20385563}; KM=40.4 uM for 3-(2-furyl)acryloyl-Phe-Met {ECO:0000269|PubMed:20385563}; KM=57.3 uM for 3-(2-furyl)acryloyl-Phe-Val {ECO:0000269|PubMed:20385563}; KM=0.329 uM for neurotensin {ECO:0000269|PubMed:20385563}; KM=9.23 uM for Met-enkephalin-Arg-Phe {ECO:0000269|PubMed:20385563};
Metal Binding:METAL 181 181 Zinc; catalytic. {ECO:0000269|PubMed:22294694}.; METAL 184 184 Zinc; catalytic. {ECO:0000269|PubMed:22294694}.; METAL 308 308 Zinc; catalytic. {ECO:0000269|PubMed:22294694}.
Induction:Up-regulated by inhibitors of histone dacetylation. {ECO:0000269|PubMed:10383164}.
Tissue Specificity:Fetal expression in the adrenal gland, brain, heart, intestine, kidney, liver and lung. Except for fetal brain that shows no imprinting, expression was found preferentially from the maternal allele.
Chemical Profile | ChEBI:Zn(2+) [CHEBI:29105]
ChEBI (Cofactor):Zn(2+) [CHEBI:29105]
Reagent Data
Name:Carboxypeptidase A4
Subcategory:Enzyme
Region:Gly 17 - Tyr 421
Molecular Weight:46.4
Source:HEK293
Species:Human
Tag:His
Format:Lyophilized
Purification System:Chromatography
Formulation:Sterile-filtered colorless solution
Formulation Concentration:1 mg/ml
Buffer Volume:Standard
Buffer Solution:PBS
pH:7.4-7.5
Toxicity
Endotoxin Level:< 1%
Aggregate Tested By:SDS-PAGE
Endotoxin Screened:< 0.1 ng/ug
Purity:> 98%
Determined: SDS-PAGE
Stain:Blue
Chromotography:Anion-exchange
Validated: RP-HPLC
Sample Handling
Storage:-20°C
Stability:This bioreagent is stable at 4°C (short-term) and -70°C(long-term). After reconstitution, sample may be stored at 4°C for 2-7 days and below -18°C for future use.
Preparation:Reconstitute in sterile distilled H2O to no less than 100 ug/ml; dilute reconstituted stock further in other aqueous solutions if needed. Please review COA for lot-specific instructions. Final measurements should be determined by the end-user for optimal performance.