BACE-1
Human Recombinant BACE1 - His Tagged
Reference ID:KB-3905
Western Blot
ELISA
FACS
Protein:Protein
Flow Cytometry
Gene of Interest
Gene Synonyms:BACE1;BACE;KIAA1149
Protein Names:Beta-secretase 1 (EC 3.4.23.46) (Aspartyl protease 2) (ASP2) (Asp 2) (Beta-site amyloid precursor protein cleaving enzyme 1) (Beta-site APP cleaving enzyme 1) (Memapsin-2) (Membrane-associated aspartic protease 2)
Accession Data
Organism:Homo sapiens (Human)
Mass (kDa):557.64
Length (aa):501
Sequence:MAQALPWLLLWMGAGVLPAHGTQHGIRLPLRSGLGGAPLGLRLPRETDEEPEEPGRRGSFVEMVDNLRGKSGQGYYVEMTVGSPPQTLNILVDTGSSNFAVGAAPHPFLHRYYQRQLSSTYRDLRKGVYVPYTQGKWEGELGTDLVSIPHGPNVTVRANIAAITESDKFFINGSNWEGILGLAYAEIARPDDSLEPFFDSLVKQTHVPNLFSLQLCGAGFPLNQSEVLASVGGSMIIGGIDHSLYTGSLWYTPIRREWYYEVIIVRVEINGQDLKMDCKEYNYDKSIVDSGTTNLRLPKKVFEAAVKSIKAASSTEKFPDGFWLGEQLVCWQAGTTPWNIFPVISLYLMGEVTNQSFRITILPQQYLRPVEDVATSQDDCYKFAISQSSTGTVMGAVIMEGFYVVFDRARKRIGFAVSACHVHDEFRTAAVEGPFVTLDMEDCGYNIPQTDESTLMTIAYVMAAICALFMLPLCLMVCQWRCLRCLRQQHDDFADDISLLK
Proteomics (Proteome ID):UP000005640
Proteomics (Chromosome): Chromosome 11
Active Site:ACT_SITE 93 93 {ECO:0000255|PROSITE-ProRule:PRU10094}.; ACT_SITE 289 289 {ECO:0000255|PROSITE-ProRule:PRU10094}.
Activity Regulation: Inhibited by RTN3 and RTN4. {ECO:0000269|PubMed:15286784, ECO:0000269|PubMed:16965550}.
Catalytic Activity: Reaction=Broad endopeptidase specificity. Cleaves Glu-Val-Asn-Leu-|-Asp-Ala-Glu-Phe in the Swedish variant of Alzheimer's amyloid precursor protein.; EC=3.4.23.46; Evidence={ECO:0000269|PubMed:10677483};
Function [CC]:Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. Cleaves APP with much more catalytic efficiency than for the wild-type. {ECO:0000269|PubMed:10656250, ECO:0000269|PubMed:10677483, ECO:0000269|PubMed:20354142}.
Kinetics:BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=15.2 mM for APP cleaved by PSEN1 (at pH4) {ECO:0000269|PubMed:10677483}; KM=1 mM for APP Swedish variant (at pH4) {ECO:0000269|PubMed:10677483}; Note=kcat is 0.67 sec(-1) and 2.45 sec(-1) for APP cleaved by PSEN1 and APP Swedish variant, respectively (PubMed:10677483). {ECO:0000269|PubMed:10677483};
Induction:Up-regulated by the Ca(2+)-regulated transcription factor NFATC4. {ECO:0000269|PubMed:25663301}.
Tissue Specificity:Expressed at high levels in the brain and pancreas. In the brain, expression is highest in the substantia nigra, locus coruleus and medulla oblongata. {ECO:0000269|PubMed:10677483, ECO:0000269|PubMed:11083922}.
Mutagenesis:MUTAGEN 93 93 D->N: Decreases beta-cleaved soluble APP production. {ECO:0000269|PubMed:10656250}.; MUTAGEN 284 284 D->N: Almost abolishes beta-cleaved soluble APP production. {ECO:0000269|PubMed:10656250}.; MUTAGEN 498 498 S->A: No effect on endocytosis from the cell surface. Increases recycling from endosomes to the cell surface. {ECO:0000269|PubMed:15615712, ECO:0000269|PubMed:15886016}.; MUTAGEN 498 498 S->D: No effect on endocytosis form the cell surface. Decreases recycling from endosomes to the cell surface. {ECO:0000269|PubMed:15886016}.; MUTAGEN 499 500 LL->AA: Impairs endocytosis and produces a delayed retrograde transport to the trans-Golgi network and delivery to the lysosmes, decreasinf its degradation. Disrupts location to late endosomes and lysosomes. Locates mainly at the cell surface. No effect on degradation regulated by GGA3. Effects on protein stability and defective internalization increases; when associated with R-501. {ECO:0000269|PubMed:15615712, ECO:0000269|PubMed:16033761, ECO:0000269|PubMed:20484053, ECO:0000269|PubMed:23109336}.; MUTAGEN 501 501 K->R: Inhibits ubiquitination. No effect on endocytosis rate. Induced protein stability and acculmulation in early and late endosomes, lysosomes and cell membrane. Effects on protein stability and defective internalization increases; when associated with A-499-500-A. {ECO:0000269|PubMed:20484053, ECO:0000269|PubMed:23109336, ECO:0000269|PubMed:27302062}.
Reagent Data
Name:BACE-1
Subcategory:Enzyme
Region:Thr 22 - Thr 457
Molecular Weight:49.2
Source:HEK293
Species:Human
Tag:His
Format:Lyophilized
Purification System:Chromatography
Formulation:Sterile-filtered colorless solution
Formulation Concentration:1 mg/ml
Buffer Volume:Standard
Buffer Solution:PBS
pH:7.4-7.5
Toxicity
Endotoxin Level:< 1%
Aggregate Tested By:SDS-PAGE
Endotoxin Screened:< 0.1 ng/ug
Purity:> 98%
Determined: SDS-PAGE
Stain:Blue
Chromotography:Anion-exchange
Validated: RP-HPLC
Sample Handling
Storage:-20°C
Stability:This bioreagent is stable at 4°C (short-term) and -70°C(long-term). After reconstitution, sample may be stored at 4°C for 2-7 days and below -18°C for future use.
Preparation:Reconstitute in sterile distilled H2O to no less than 100 ug/ml; dilute reconstituted stock further in other aqueous solutions if needed. Please review COA for lot-specific instructions. Final measurements should be determined by the end-user for optimal performance.