Mouse Recombinant Pdcd1 - Fc Tagged
Reference ID:KB-3817
Western Blot
Flow Cytometry
Gene of Interest
Gene Synonyms:Pdcd1;Pd1
Protein Names:Programmed cell death protein 1 (Protein PD-1) (mPD-1) (CD antigen CD279)
Accession Data
Organism:Mus musculus (Mouse)
Mass (kDa):318.42
Length (aa):288
Proteomics (Proteome ID):UP000000589
Proteomics (Chromosome): Chromosome 1
Function [CC]:Inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self (PubMed:10485649, PubMed:11698646, PubMed:11209085, PubMed:21300912). Delivers inhibitory signals upon binding to ligands, such as CD274/PDCD1L1 and CD273/PDCD1LG2 (PubMed:11015443, PubMed:11224527, PubMed:22641383, PubMed:18287011, PubMed:18641123). Following T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation (PubMed:22641383). Suppresses T-cell activation through the recruitment of PTPN11/SHP-2: following ligand-binding, PDCD1 is phosphorylated within the ITSM motif, leading to the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta (PubMed:11698646, PubMed:22641383). The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and facilitate tumor survival (By similarity). {ECO:0000250|UniProtKB:Q15116, ECO:0000269|PubMed:10485649, ECO:0000269|PubMed:11015443, ECO:0000269|PubMed:11209085, ECO:0000269|PubMed:11224527, ECO:0000269|PubMed:11698646, ECO:0000269|PubMed:18287011, ECO:0000269|PubMed:18641123, ECO:0000269|PubMed:21300912, ECO:0000269|PubMed:22641383}.
Developmental Stage:Induced at programmed cell death. {ECO:0000269|PubMed:1396582}.
Tissue Specificity:Thymus-specific. {ECO:0000269|PubMed:1396582}.
Mutagenesis:MUTAGEN 225 225 Y->F: Reduced tyrosine phosphorylation. Decreased ability to mediate recruitment of PTPN11/SHP-2. {ECO:0000269|PubMed:11698646, ECO:0000269|PubMed:22641383}.; MUTAGEN 248 248 Y->F: Reduced tyrosine phosphorylation. Abolished ability to mediate recruitment of PTPN11/SHP-2. {ECO:0000269|PubMed:11698646, ECO:0000269|PubMed:22641383}.
Reagent Data
Class:Immune Checkpoint
Region:Leu 25 - Gln 167
Molecular Weight:43.2
Conjugation:Lysine side chain Biotin
Purification System:Chromatography
Formulation:Sterile-filtered colorless solution
Formulation Concentration:1 mg/ml
Buffer Volume:Standard
Buffer Solution:PBS
Endotoxin Level:< 1%
Aggregate Tested By:SDS-PAGE
Endotoxin Screened:< 0.1 ng/ug
Purity:> 98%
Determined: SDS-PAGE
Validated: RP-HPLC
Sample Handling
Stability:This bioreagent is stable at 4°C (short-term) and -70°C(long-term). After reconstitution, sample may be stored at 4°C for 2-7 days and below -18°C for future use.
Preparation:Reconstitute in sterile distilled H2O to no less than 100 ug/ml; dilute reconstituted stock further in other aqueous solutions if needed. Please review COA for lot-specific instructions. Final measurements should be determined by the end-user for optimal performance.