LAG-3
Cynomolgus Recombinant Lag3 - Fc Tagged
Reference ID:KB-3800
Western Blot
ELISA
FACS
Protein:Protein
Flow Cytometry
Gene of Interest
Gene Synonyms:Lag3
Protein Names:Lymphocyte activation gene 3 protein (LAG-3) (Activation-induced cytidine deaminase-linked autoimmunity protein) (Aida) (CD antigen CD223) [Cleaved into: Secreted lymphocyte activation gene 3 protein (sLAG-3)]
Accession Data
Organism:Mus musculus (Mouse)
Mass (kDa):569.78
Length (aa):521
Sequence:MREDLLLGFLLLGLLWEAPVVSSGPGKELPVVWAQEGAPVHLPCSLKSPNLDPNFLRRGGVIWQHQPDSGQPTPIPALDLHQGMPSPRQPAPGRYTVLSVAPGGLRSGRQPLHPHVQLEERGLQRGDFSLWLRPALRTDAGEYHATVRLPNRALSCSLRLRVGQASMIASPSGVLKLSDWVLLNCSFSRPDRPVSVHWFQGQNRVPVYNSPRHFLAETFLLLPQVSPLDSGTWGCVLTYRDGFNVSITYNLKVLGLEPVAPLTVYAAEGSRVELPCHLPPGVGTPSLLIAKWTPPGGGPELPVAGKSGNFTLHLEAVGLAQAGTYTCSIHLQGQQLNATVTLAVITVTPKSFGLPGSRGKLLCEVTPASGKERFVWRPLNNLSRSCPGPVLEIQEARLLAERWQCQLYEGQRLLGATVYAAESSSGAHSARRISGDLKGGHLVLVLILGALSLFLLVAGAFGFHWWRKQLLLRRFSALEHGIQPFPAQRKIEELERELETEMGQEPEPEPEPQLEPEPRQL
Proteomics (Proteome ID):UP000000589
Proteomics (Chromosome): Chromosome 6
Function [CC]:Lymphocyte activation gene 3 protein: Inhibitory receptor on antigen activated T-cells (PubMed:12209638, PubMed:12421911, PubMed:12672063, PubMed:15100286, PubMed:15634887, PubMed:30580966). Delivers inhibitory signals upon binding to ligands, such as FGL1 (PubMed:30580966). FGL1 constitutes a major ligand of LAG3 and is responsible for LAG3 T-cell inhibitory function (PubMed:30580966). Following TCR engagement, LAG3 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation (PubMed:12209638, PubMed:12421911, PubMed:12672063, PubMed:15100286, PubMed:15634887). May inhibit antigen-specific T-cell activation in synergy with PDCD1/PD-1, possibly by acting as a coreceptor for PDCD1/PD-1 (PubMed:21300912). Negatively regulates the proliferation, activation, effector function and homeostasis of both CD8(+) and CD4(+) T-cells (PubMed:12209638, PubMed:12421911, PubMed:12672063, PubMed:15100286, PubMed:15634887). Also mediates immune tolerance: constitutively expressed on a subset of regulatory T-cells (Tregs) and contributes to their suppressive function (PubMed:15485628). Also acts as a negative regulator of plasmacytoid dendritic cell (pDCs) activation (PubMed:19201841). Binds MHC class II (MHC-II); the precise role of MHC-II-binding is however unclear (PubMed:12209638, PubMed:12421911, PubMed:15634887). {ECO:0000269|PubMed:12209638, ECO:0000269|PubMed:12421911, ECO:0000269|PubMed:12672063, ECO:0000269|PubMed:15100286, ECO:0000269|PubMed:15485628, ECO:0000269|PubMed:15634887, ECO:0000269|PubMed:19201841, ECO:0000269|PubMed:21300912, ECO:0000269|PubMed:30580966}.; Secreted lymphocyte activation gene 3 protein: May function as a ligand for MHC class II (MHC-II) on antigen-presenting cells (APC), promoting APC activation/maturation and driving Th1 immune response. {ECO:0000269|PubMed:11937541}.
Tissue Specificity:Primarily expressed in activated CD4(+) and CD8(+) T-cells (PubMed:12209638). Also expressed in a subset of regulatory T-cells (Tregs), such as natural CD4(+)CD25(+) Tregs (PubMed:15485628). Also expressed on plasmacytoid dendritic cells (pDCs) (PubMed:19201841). {ECO:0000269|PubMed:12209638, ECO:0000269|PubMed:15485628, ECO:0000269|PubMed:19201841}.
Mutagenesis:MUTAGEN 94 94 R->E: Decreased binding to MHC class II. {ECO:0000269|PubMed:12421911}.; MUTAGEN 95 95 Y->F: No significant effect on MHC class II-binding. {ECO:0000269|PubMed:12421911}.; MUTAGEN 121 121 R->A: No significant effect on MHC class II-binding. {ECO:0000269|PubMed:12421911}.; MUTAGEN 490 490 K->A: Abolished ability to inhibit T-cell activation. {ECO:0000269|PubMed:12421911}.
Reagent Data
Name:LAG-3
Class:Immune Checkpoint
Subcategory:Protein
Region:Ala 18 - His 449
Molecular Weight:73.7
Source:HEK293
Species:Cynomolgus
Tag:Fc
Format:Lyophilized
Purification System:Chromatography
Formulation:Sterile-filtered colorless solution
Formulation Concentration:1 mg/ml
Buffer Volume:Standard
Buffer Solution:PBS
pH:7.4-7.5
Toxicity
Endotoxin Level:< 1%
Aggregate Tested By:SDS-PAGE
Endotoxin Screened:< 0.1 ng/ug
Purity:> 98%
Determined: SDS-PAGE
Stain:Blue
Chromotography:Anion-exchange
Validated: RP-HPLC
Sample Handling
Storage:-20°C
Stability:This bioreagent is stable at 4°C (short-term) and -70°C(long-term). After reconstitution, sample may be stored at 4°C for 2-7 days and below -18°C for future use.
Preparation:Reconstitute in sterile distilled H2O to no less than 100 ug/ml; dilute reconstituted stock further in other aqueous solutions if needed. Please review COA for lot-specific instructions. Final measurements should be determined by the end-user for optimal performance.