CD47
Rat Recombinant Cd47 - Fc Tagged
Reference ID:KB-3770
Western Blot
ELISA
FACS
Protein:Protein
Flow Cytometry
Gene of Interest
Gene Synonyms:Cd47
Protein Names:Leukocyte surface antigen CD47 (Integrin-associated protein) (IAP) (CD antigen CD47)
Accession Data
Organism:Rattus norvegicus (Rat)
Mass (kDa):329.95
Length (aa):303
Sequence:MWPLAAALLLGSCCCGSAQLLLSKVKSVEFTSCNDTVVIPCKVLNVEAQSTDEMFVKWKLNKSYIFIYDGNKNSTTREQNFTSAKISVSDLLKGIASLTMDTHEAVVGNYTCEVTELSREGKTVIELKNRPVSWFSTNEKILIVIFPILAILLFWGKFGILTLKYKSSHTNKRIILLLVAGLALTLIVVVGAILFIPGEKPVKNASGLGLIVISTGILILLQYNVFMTAFGMTSFTIAILITQVLGYVLAVVGMCLCIMACEPVHGPLLISGLGIIALAELLGLVYMKFVASNQRTIQPPRNN
Proteomics (Proteome ID):UP000002494
Proteomics (Chromosome): Chromosome 11
Function [CC]:Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection (By similarity). Plays an important role in memory formation and synaptic plasticity in the hippocampus. {ECO:0000250, ECO:0000269|PubMed:9592107}.
Induction:By ferric nitrilotriacetate, NMDA and amphetamine. {ECO:0000269|PubMed:9119739, ECO:0000269|PubMed:9592107}.
Tissue Specificity:Expressed in hippocampus. {ECO:0000269|PubMed:9592107}.
Reagent Data
Name:CD47
Class:Immune Checkpoint
Subcategory:Protein
Region:Gln 19 - Lys 140
Molecular Weight:40.4
Source:HEK293
Species:Rat
Tag:Fc
Format:Lyophilized
Purification System:Chromatography
Formulation:Sterile-filtered colorless solution
Formulation Concentration:1 mg/ml
Buffer Volume:Standard
Buffer Solution:PBS
pH:7.4-7.5
Toxicity
Endotoxin Level:< 1%
Aggregate Tested By:SDS-PAGE
Endotoxin Screened:< 0.1 ng/ug
Purity:> 98%
Determined: SDS-PAGE
Stain:Blue
Chromotography:Anion-exchange
Validated: RP-HPLC
Sample Handling
Storage:-20°C
Stability:This bioreagent is stable at 4°C (short-term) and -70°C(long-term). After reconstitution, sample may be stored at 4°C for 2-7 days and below -18°C for future use.
Preparation:Reconstitute in sterile distilled H2O to no less than 100 ug/ml; dilute reconstituted stock further in other aqueous solutions if needed. Please review COA for lot-specific instructions. Final measurements should be determined by the end-user for optimal performance.