Human Recombinant PVR - Fc Tagged
Reference ID:KB-3745
Western Blot
Flow Cytometry
Gene of Interest
Gene Synonyms:PVR;PVS
Protein Names:Poliovirus receptor (Nectin-like protein 5) (NECL-5) (CD antigen CD155)
Accession Data
Organism:Homo sapiens (Human)
Mass (kDa):453.03
Length (aa):417
Proteomics (Proteome ID):UP000005640
Proteomics (Chromosome): Chromosome 19
Function [CC]:Mediates NK cell adhesion and triggers NK cell effector functions. Binds two different NK cell receptors: CD96 and CD226. These interactions accumulates at the cell-cell contact site, leading to the formation of a mature immunological synapse between NK cell and target cell. This may trigger adhesion and secretion of lytic granules and IFN-gamma and activate cytoxicity of activated NK cells. May also promote NK cell-target cell modular exchange, and PVR transfer to the NK cell. This transfer is more important in some tumor cells expressing a lot of PVR, and may trigger fratricide NK cell activation, providing tumors with a mechanism of immunoevasion. Plays a role in mediating tumor cell invasion and migration. {ECO:0000269|PubMed:15471548, ECO:0000269|PubMed:15607800}.; (Microbial infection) Acts as a receptor for poliovirus. May play a role in axonal transport of poliovirus, by targeting virion-PVR-containing endocytic vesicles to the microtubular network through interaction with DYNLT1. This interaction would drive the virus-containing vesicle to the axonal retrograde transport. {ECO:0000269|PubMed:2538245}.; (Microbial infection) Acts as a receptor for Pseudorabies virus. {ECO:0000269|PubMed:9616127}.; (Microbial infection) Is prevented to reach cell surface upon infection by Human cytomegalovirus /HHV-5, presumably to escape immune recognition of infected cell by NK cells. {ECO:0000269|PubMed:15640804}.
Kinetics:BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=72 nM for VTN;
Induction:Transcriptionally regulated by SHH. {ECO:0000269|PubMed:11983699}.
Mutagenesis:MUTAGEN 369 372 KCSR->ACSA: Partial loss of DYNLT1 binding. {ECO:0000269|PubMed:15194795}.
Miscellaneous [CC]:The V-type domain is necessary and sufficient for virus binding and uptake.
Reagent Data
Class:Immune Checkpoint
Region:Trp 21 - Asn 343
Molecular Weight:61.7
Purification System:Chromatography
Formulation:Sterile-filtered colorless solution
Formulation Concentration:1 mg/ml
Buffer Volume:Standard
Buffer Solution:PBS
Endotoxin Level:< 1%
Aggregate Tested By:SDS-PAGE
Endotoxin Screened:< 0.1 ng/ug
Purity:> 98%
Determined: SDS-PAGE
Validated: RP-HPLC
Sample Handling
Stability:This bioreagent is stable at 4°C (short-term) and -70°C(long-term). After reconstitution, sample may be stored at 4°C for 2-7 days and below -18°C for future use.
Preparation:Reconstitute in sterile distilled H2O to no less than 100 ug/ml; dilute reconstituted stock further in other aqueous solutions if needed. Please review COA for lot-specific instructions. Final measurements should be determined by the end-user for optimal performance.