Human Recombinant OLFM4 - His Tagged
Reference ID:KB-3687
Western Blot
Flow Cytometry
Gene of Interest
Gene Synonyms:OLFM4;GW112;UNQ362/PRO698
Protein Names:Olfactomedin-4 (OLM4) (Antiapoptotic protein GW112) (G-CSF-stimulated clone 1 protein) (hGC-1) (hOLfD)
Accession Data
Organism:Homo sapiens (Human)
Mass (kDa):572.80
Length (aa):510
Proteomics (Proteome ID):UP000005640
Proteomics (Chromosome): Chromosome 13
Function [CC]:May promote proliferation of pancreatic cancer cells by favoring the transition from the S to G2/M phase. In myeloid leukemic cell lines, inhibits cell growth and induces cell differentiation and apoptosis. May play a role in the inhibition of EIF4EBP1 phosphorylation/deactivation. Facilitates cell adhesion, most probably through interaction with cell surface lectins and cadherin. {ECO:0000269|PubMed:16566923, ECO:0000269|PubMed:17270022, ECO:0000269|PubMed:20724538}.
Developmental Stage:Elevated expression during the early S phase of the cell cycle, followed by a gradual decrease during late S phase. {ECO:0000269|PubMed:17270022}.
Induction:By retinoic acid. This induction requires functional NFKB pathway. {ECO:0000269|PubMed:20724538}.
Tissue Specificity:Expressed during myeloid lineage development. Much higher expression in bone marrow neutrophils than in peripheral blood neutrophils (at protein level). Strongly expressed in the prostate, small intestine and colon and moderately expressed in the bone marrow and stomach. Overexpressed in some pancreatic cancer tissues. {ECO:0000269|PubMed:11867215, ECO:0000269|PubMed:16566923, ECO:0000269|PubMed:17270022, ECO:0000269|PubMed:20724538}.
Mutagenesis:MUTAGEN 83 83 C->A: Abolishes secretion. No effect on multimer formation. {ECO:0000269|PubMed:16566923}.; MUTAGEN 85 85 C->A: Abolishes secretion. No effect on multimer formation. {ECO:0000269|PubMed:16566923}.; MUTAGEN 226 226 C->A: No effect on secretion. Affects multimer formation. {ECO:0000269|PubMed:16566923}.; MUTAGEN 246 246 C->A: Abolishes secretion. No effect on multimer formation. {ECO:0000269|PubMed:16566923}.; MUTAGEN 437 437 C->A: Abolishes secretion. No effect on multimer formation. {ECO:0000269|PubMed:16566923}.
Reagent Data
Class:Growth Factor
Region:Asp 21 - Gln 510
Molecular Weight:56
Purification System:Chromatography
Formulation:Sterile-filtered colorless solution
Formulation Concentration:1 mg/ml
Buffer Volume:Standard
Buffer Solution:PBS
Endotoxin Level:< 1%
Aggregate Tested By:SDS-PAGE
Endotoxin Screened:< 0.1 ng/ug
Purity:> 98%
Determined: SDS-PAGE
Validated: RP-HPLC
Sample Handling
Stability:This bioreagent is stable at 4°C (short-term) and -70°C(long-term). After reconstitution, sample may be stored at 4°C for 2-7 days and below -18°C for future use.
Preparation:Reconstitute in sterile distilled H2O to no less than 100 ug/ml; dilute reconstituted stock further in other aqueous solutions if needed. Please review COA for lot-specific instructions. Final measurements should be determined by the end-user for optimal performance.