Human Recombinant TF - His Tagged
Reference ID:KB-3677
Western Blot
Flow Cytometry
Gene of Interest
Gene Synonyms:TF;PRO1400
Protein Names:Serotransferrin (Transferrin) (Beta-1 metal-binding globulin) (Siderophilin)
Accession Data
Organism:Homo sapiens (Human)
Mass (kDa):770.64
Length (aa):698
Proteomics (Proteome ID):UP000005640
Proteomics (Chromosome): Chromosome 3
Polymorphism: Different polymorphic variants of transferrin are known. The sequence shown is the predominant electrophoretic variant (C1 or TF*C1).
Binding Site:BINDING 139 139 Carbonate 1.; BINDING 143 143 Carbonate 1.; BINDING 145 145 Carbonate 1; via amide nitrogen.; BINDING 146 146 Carbonate 1; via amide nitrogen.; BINDING 471 471 Carbonate 2. {ECO:0000255|PROSITE-ProRule:PRU00741}.; BINDING 475 475 Carbonate 2. {ECO:0000255|PROSITE-ProRule:PRU00741}.; BINDING 477 477 Carbonate 2; via amide nitrogen. {ECO:0000255|PROSITE-ProRule:PRU00741}.; BINDING 478 478 Carbonate 2; via amide nitrogen. {ECO:0000255|PROSITE-ProRule:PRU00741}.
Function [CC]:Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
Metal Binding:METAL 82 82 Iron 1.; METAL 114 114 Iron 1.; METAL 207 207 Iron 1.; METAL 268 268 Iron 1.; METAL 411 411 Iron 2. {ECO:0000255|PROSITE-ProRule:PRU00741}.; METAL 445 445 Iron 2. {ECO:0000255|PROSITE-ProRule:PRU00741}.; METAL 536 536 Iron 2. {ECO:0000255|PROSITE-ProRule:PRU00741}.; METAL 604 604 Iron 2. {ECO:0000255|PROSITE-ProRule:PRU00741}.
Tissue Specificity:Expressed by the liver and secreted in plasma.
Disease:Atransferrinemia (ATRAF) [MIM:209300]: A rare autosomal recessive disorder characterized by abnormal synthesis of transferrin leading to iron overload and microcytic hypochromic anemia. {ECO:0000269|PubMed:11110675, ECO:0000269|PubMed:15466165}. Note=The disease is caused by mutations affecting the gene represented in this entry.
Reagent Data
Region:Val 20 - Pro 698
Molecular Weight:76
Purification System:Chromatography
Formulation:Sterile-filtered colorless solution
Formulation Concentration:1 mg/ml
Buffer Volume:Standard
Buffer Solution:PBS
Endotoxin Level:< 1%
Aggregate Tested By:SDS-PAGE
Endotoxin Screened:< 0.1 ng/ug
Purity:> 98%
Determined: SDS-PAGE
Validated: RP-HPLC
Sample Handling
Stability:This bioreagent is stable at 4°C (short-term) and -70°C(long-term). After reconstitution, sample may be stored at 4°C for 2-7 days and below -18°C for future use.
Preparation:Reconstitute in sterile distilled H2O to no less than 100 ug/ml; dilute reconstituted stock further in other aqueous solutions if needed. Please review COA for lot-specific instructions. Final measurements should be determined by the end-user for optimal performance.