Cytokines
GFER
Recombinant ID:
3594
Gene of Interest
Gene Synonyms:
GFER;ALR;HERV1;HPO
Protein Names:
FAD-linked sulfhydryl oxidase ALR (EC 1.8.3.2) (Augmenter of liver regeneration) (hERV1) (Hepatopoietin)
Accession Data
Organism:
Homo sapiens (Human)
Mass (kDa):
23449
Length (aa):
205
Proteomics (Proteome ID):
FAD-linked sulfhydryl oxidase ALR (EC 1.8.3.2) (Augmenter of liver regeneration) (hERV1) (Hepatopoietin)
Proteomics (Chromosome):
UP000005640
Sequence:
MAAPGERGRFHGGNLFFLPGGARSEMMDDLATDARGRGAGRRDAAASASTPAQAPTSDSPVAEDASRRRPCRACVDFKTWMRTQQKRDTKFREDCPPDREELGRHSWAVLHTLAAYYPDLPTPEQQQDMAQFIHLFSKFYPCEECAEDLRKRLCRNHPDTRTRACFTQWLCHLHNEVNRKLGKPDFDCSKVDERWRDGWKDGSCD
Analysis Summary:
Isoform 1: FAD-dependent sulfhydryl oxidase that regenerates the redox-active disulfide bonds in CHCHD4/MIA40, a chaperone essential for disulfide bond formation and protein folding in the mitochondrial intermembrane space. The reduced form of CHCHD4/MIA40 forms a transient intermolecular disulfide bridge with GFER/ERV1, resulting in regeneration of the essential disulfide bonds in CHCHD4/MIA40, while GFER/ERV1 becomes re-oxidized by donating electrons to cytochrome c or molecular oxygen. {ECO:0000269|PubMed:19397338, ECO:0000269|PubMed:20593814, ECO:0000269|PubMed:21383138, ECO:0000269|PubMed:22224850, ECO:0000269|PubMed:23186364, ECO:0000269|PubMed:23676665}.; Isoform 2: May act as an autocrine hepatotrophic growth factor promoting liver regeneration.,Alternative sequence (1); Beta strand (1); Binding site (2); Chain (1); Disulfide bond (4); Domain (1); Erroneous initiation (4); Frameshift (1); Helix (7); Modified residue (1); Natural variant (2); Nucleotide binding (3); Sequence conflict (3),Ubiquitously expressed. Highest expression in the testis and liver and low expression in the muscle. {ECO:0000269|PubMed:19409522}.,H2O2 [CHEBI:16240]; O2 [CHEBI:15379]; R'C(R)S-S(R)CR' [CHEBI:17412]; R'C(R)SH [CHEBI:16520]; FAD [CHEBI:57692],H2O2 [CHEBI:16240]; O2 [CHEBI:15379]; R'C(R)S-S(R)CR' [CHEBI:17412]; R'C(R)SH [CHEBI:16520],FAD [CHEBI:57692],Myopathy, mitochondrial progressive, with congenital cataract, hearing loss and developmental delay (MPMCHD) [MIM:613076]: A disease characterized by progressive myopathy and partial combined respiratory-chain deficiency, congenital cataract, sensorineural hearing loss, and developmental delay. {ECO:0000269|PubMed:19409522, ECO:0000269|PubMed:20593814}. Note=The disease is caused by mutations affecting the gene represented in this entry.
Reagent Data
Name:
FAD-linked sulfhydryl oxidase ALR (EC 1.8.3.2) (Augmenter of liver regeneration) (hERV1) (Hepatopoietin)
Subcategory:
Recombinant
Amino Acid Sequence:
Inquire
Species
Human
Tag:
Format:
Lyophilized
pH:
7.4-7.5
Formulation:
Sterile-filtered colorless solution
Buffer Volume
Standard
Buffer Solution:
PBS
Metal Chelating Agents
Purity:
> 98%
Determined:
SDS-PAGE
Stained:
Assay (Variable)
Validated:
RP-HPLC
Sample Handling
Storage:
-20°C
Stability:
This bioreagent is stable at 4°C (short-term) and -70°C(long-term). After reconstitution, sample may be stored at 4°C for 2-7 days and below -18°C for future use.
Preparation:
Reconstitute in sterile distilled H2O to no less than 100ug/ml; dilute reconstituted stock further in other aqueous solutions if needed. Please review COA for lot-specific instructions. Final measurements should be determined by the end-user for optimal performance.
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