CX3CL1
Human Recombinant CX3CL1 - His Tagged
Reference ID:KB-3585
Western Blot
ELISA
FACS
Protein:Protein
Flow Cytometry
Gene of Interest
Gene Synonyms:CX3CL1;FKN;NTT;SCYD1;A-152E5.2
Protein Names:Fractalkine (C-X3-C motif chemokine 1) (CX3C membrane-anchored chemokine) (Neurotactin) (Small-inducible cytokine D1) [Cleaved into: Processed fractalkine]
Accession Data
Organism:Homo sapiens (Human)
Mass (kDa):422.03
Length (aa):397
Sequence:MAPISLSWLLRLATFCHLTVLLAGQHHGVTKCNITCSKMTSKIPVALLIHYQQNQASCGKRAIILETRQHRLFCADPKEQWVKDAMQHLDRQAAALTRNGGTFEKQIGEVKPRTTPAAGGMDESVVLEPEATGESSSLEPTPSSQEAQRALGTSPELPTGVTGSSGTRLPPTPKAQDGGPVGTELFRVPPVSTAATWQSSAPHQPGPSLWAEAKTSEAPSTQDPSTQASTASSPAPEENAPSEGQRVWGQGQSPRPENSLEREEMGPVPAHTDAFQDWGPGSMAHVSVVPVSSEGTPSREPVASGSWTPKAEEPIHATMDPQRLGVLITPVPDAQAATRRQAVGLLAFLGLLFCLGVAMFTYQSLQGCPRKMAGEMAEGLRYIPRSCGSNSYVLVPV
Proteomics (Proteome ID):UP000005640
Proteomics (Chromosome): Chromosome 16
Function [CC]:Acts as a ligand for both CX3CR1 and integrins. Binds to CX3CR1 (PubMed:23125415, PubMed:9931005, PubMed:21829356). Binds to integrins ITGAV:ITGB3 and ITGA4:ITGB1. Can activate integrins in both a CX3CR1-dependent and CX3CR1-independent manner. In the presence of CX3CR1, activates integrins by binding to the classical ligand-binding site (site 1) in integrins. In the absence of CX3CR1, binds to a second site (site 2) in integrins which is distinct from site 1 and enhances the binding of other integrin ligands to site 1 (PubMed:23125415, PubMed:24789099). The soluble form is chemotactic for T-cells and monocytes and not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium (PubMed:9024663, PubMed:9177350). {ECO:0000269|PubMed:21829356, ECO:0000269|PubMed:23125415, ECO:0000269|PubMed:24789099, ECO:0000269|PubMed:9024663, ECO:0000269|PubMed:9177350, ECO:0000269|PubMed:9931005}.
Site:SITE 339 340 Cleavage; to produce soluble form. {ECO:0000255}.
Induction:By TNF and IL1/interleukin-1 in pulmonary endothelial cells and umbilical vein endothelial cells. {ECO:0000269|PubMed:9024663}.
Tissue Specificity:Expressed in the seminal plasma, endometrial fluid and follicular fluid (at protein level). Small intestine, colon, testis, prostate, heart, brain, lung, skeletal muscle, kidney and pancreas. Most abundant in the brain and heart. {ECO:0000269|PubMed:23765988, ECO:0000269|PubMed:9024663, ECO:0000269|PubMed:9177350}.
Mutagenesis:MUTAGEN 60 60 K->A: Reduced binding to integrin ITGAV:ITGB3, but no effect on binding to CX3CR1; when associated with A-61. {ECO:0000269|PubMed:23125415}.; MUTAGEN 60 60 K->E: Dominant-negative mutant, reduced binding to integrins ITGAV:ITGB3 and ITGA4:ITGB1, no effect on binding to CX3CR1, defective in ternary complex formation, integrin activation and fractalkine signaling and suppression of leukocyte recruitment in the peritonitis model in vivo; when associated with E-61. {ECO:0000269|PubMed:23125415, ECO:0000269|PubMed:24789099}.; MUTAGEN 61 61 R->A: Reduced binding to integrin ITGAV:ITGB3, but no effect on binding to CX3CR1; when associated with A-60. {ECO:0000269|PubMed:23125415}.; MUTAGEN 61 61 R->E: Dominant-negative mutant, reduced binding to integrins ITGAV:ITGB3 and ITGA4:ITGB1, no effect on binding to CX3CR1, defective in ternary complex formation, integrin activation and fractalkine signaling and suppression of leukocyte recruitment in the peritonitis model in vivo; when associated with E-60. {ECO:0000269|PubMed:23125415, ECO:0000269|PubMed:24789099}.; MUTAGEN 71 71 R->A: Loss of binding to CX3CR1 and ability to induce chemotaxis but no effect on binding to integrins. {ECO:0000269|PubMed:23125415}.; MUTAGEN 78 78 K->A: Little or no effect on binding to integrin ITGAV:ITGB3. {ECO:0000269|PubMed:23125415}.; MUTAGEN 78 78 K->A: Little or no effect on binding to integrin ITGAV:ITGB3; when associated with A-83. {ECO:0000269|PubMed:23125415}.; MUTAGEN 83 83 K->A: Little or no effect on binding to integrin ITGAV:ITGB3; when associated with A-78. {ECO:0000269|PubMed:23125415}.
Reagent Data
Name:CX3CL1
Class:Chemokine
Subcategory:Protein
Region:Gln 25 - Gln 341
Molecular Weight:35.5
Source:HEK293
Species:Human
Tag:His
Format:Lyophilized
Purification System:Chromatography
Formulation:Sterile-filtered colorless solution
Formulation Concentration:1 mg/ml
Buffer Volume:Standard
Buffer Solution:PBS
pH:7.4-7.5
Toxicity
Endotoxin Level:< 1%
Aggregate Tested By:SDS-PAGE
Endotoxin Screened:< 0.1 ng/ug
Purity:> 98%
Determined: SDS-PAGE
Stain:Blue
Chromotography:Anion-exchange
Validated: RP-HPLC
Sample Handling
Storage:-20°C
Stability:This bioreagent is stable at 4°C (short-term) and -70°C(long-term). After reconstitution, sample may be stored at 4°C for 2-7 days and below -18°C for future use.
Preparation:Reconstitute in sterile distilled H2O to no less than 100 ug/ml; dilute reconstituted stock further in other aqueous solutions if needed. Please review COA for lot-specific instructions. Final measurements should be determined by the end-user for optimal performance.