Csf1r;Csfmr;Fms

Macrophage colony-stimulating factor 1 receptor (CSF-1 receptor) (CSF-1-R) (CSF-1R) (M-CSF-R) (EC 2.7.10.1) (Proto-oncogene c-Fms) (CD antigen CD115)

Mus musculus (Mouse)

109179

977

Pending Verification

Macrophage colony-stimulating factor 1 receptor (CSF-1 receptor) (CSF-1-R) (CSF-1R) (M-CSF-R) (EC 2.7.10.1) (Proto-oncogene c-Fms) (CD antigen CD115)

N/A

MUTAGEN 544 544 Y->F: No effect on binding to THOC5. {ECO:0000269|PubMed:10597251}.; MUTAGEN 559 559 Y->F: Reduced interaction with CBL. Prolonged signaling, due to reduced internalization and degradation. Reduced interaction with FYN. Promotes cell proliferation. Reduced autophosphorylation at Tyr-807. {ECO:0000269|PubMed:15297464, ECO:0000269|PubMed:17420255, ECO:0000269|PubMed:8007983}.; MUTAGEN 614 614 K->A: Loss of kinase activity. {ECO:0000269|PubMed:10597251, ECO:0000269|PubMed:8262059, ECO:0000269|PubMed:9312046}.; MUTAGEN 614 614 K->M: Loss of kinase activity. Abolishes binding to THOC5. {ECO:0000269|PubMed:10597251, ECO:0000269|PubMed:8262059, ECO:0000269|PubMed:9312046}.; MUTAGEN 697 697 Y->F: Abolishes interaction with GRB2. {ECO:0000269|PubMed:8262059, ECO:0000269|PubMed:9312046}.; MUTAGEN 706 706 Y->F: No effect on binding to THOC5. Slightly reduced enhancement of cell proliferation. {ECO:0000269|PubMed:10597251, ECO:0000269|PubMed:1652061}.; MUTAGEN 706 706 Y->G: Slightly impaired signaling. {ECO:0000269|PubMed:10597251, ECO:0000269|PubMed:1652061}.; MUTAGEN 721 721 Y->F: Abolishes interaction with PIK3R1. Strongly reduced phosphorylation of PLCG2. No effect on binding to THOC5. {ECO:0000269|PubMed:10597251, ECO:0000269|PubMed:21610095, ECO:0000269|PubMed:8262059, ECO:0000269|PubMed:9312046}.; MUTAGEN 807 807 Y->F: Reduced kinase activity. Strongly reduced phosphorylation of PLCG2. Diminishes binding to THOC5. {ECO:0000269|PubMed:10597251, ECO:0000269|PubMed:1652061, ECO:0000269|PubMed:9312046}.; MUTAGEN 807 807 Y->G: May alter protein folding or stability. Loss of kinase activity. No effect on interaction with PIK3R1. {ECO:0000269|PubMed:10597251, ECO:0000269|PubMed:1652061, ECO:0000269|PubMed:9312046}.

MELGPPLVLLLATVWHGQGAPVIEPSGPELVVEPGETVTLRCVSNGSVEWDGPISPYWTLDPESPGSTLTTRNATFKNTGTYRCTELEDPMAGSTTIHLYVKDPAHSWNLLAQEVTVVEGQEAVLPCLITDPALKDSVSLMREGGRQVLRKTVYFFSPWRGFIIRKAKVLDSNTYVCKTMVNGRESTSTGIWLKVNRVHPEPPQIKLEPSKLVRIRGEAAQIVCSATNAEVGFNVILKRGDTKLEIPLNSDFQDNYYKKVRALSLNAVDFQDAGIYSCVASNDVGTRTATMNFQVVESAYLNLTSEQSLLQEVSVGDSLILTVHADAYPSIQHYNWTYLGPFFEDQRKLEFITQRAIYRYTFKLFLNRVKASEAGQYFLMAQNKAGWNNLTFELTLRYPPEVSVTWMPVNGSDVLFCDVSGYPQPSVTWMECRGHTDRCDEAQALQVWNDTHPEVLSQKPFDKVIIQSQLPIGTLKHNMTYFCKTHNSVGNSSQYFRAVSLGQSKQLPDESLFTPVVVACMSVMSLLVLLLLLLLYKYKQKPKYQVRWKIIERYEGNSYTFIDPTQLPYNEKWEFPRNNLQFGKTLGAGAFGKVVEATAFGLGKEDAVLKVAVKMLKSTAHADEKEALMSELKIMSHLGQHENIVNLLGACTHGGPVLVITEYCCYGDLLNFLRRKAEAMLGPSLSPGQDSEGDSSYKNIHLEKKYVRRDSGFSSQGVDTYVEMRPVSTSSSDSFFKQDLDKEASRPLELWDLLHFSSQVAQGMAFLASKNCIHRDVAARNVLLTSGHVAKIGDFGLARDIMNDSNYVVKGNARLPVKWMAPESIFDCVYTVQSDVWSYGILLWEIFSLGLNPYPGILVNNKFYKLVKDGYQMAQPVFAPKNIYSIMQSCWDLEPTRRPTFQQICFLLQEQARLERRDQDYANLPSSGGSSGSDSGGGSSGGSSSEPEEESSSEHLACCEPGDIAQPLLQPNNYQFC

Tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines in response to IL34 and CSF1, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone and tooth development. Required for normal male and female fertility, and for normal development of milk ducts and acinar structures in the mammary gland during pregnancy. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration, and promotes cancer cell invasion. Activates several signaling pathways in response to ligand binding. Phosphorylates PIK3R1, PLCG2, GRB2, SLA2 and CBL. Activation of PLCG2 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, that then lead to the activation of protein kinase C family members, especially PRKCD. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leads to activation of the AKT1 signaling pathway. Activated CSF1R also mediates activation of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1, and of the SRC family kinases SRC, FYN and YES1. Activated CSF1R transmits signals both via proteins that directly interact with phosphorylated tyrosine residues in its intracellular domain, or via adapter proteins, such as GRB2. Promotes activation of STAT family members STAT3, STAT5A and/or STAT5B. Promotes tyrosine phosphorylation of SHC1 and INPP5D/SHIP-1. Receptor signaling is down-regulated by protein phosphatases, such as INPP5D/SHIP-1, that dephosphorylate the receptor and its downstream effectors, and by rapid internalization of the activated receptor. {ECO:0000269|PubMed:10958675, ECO:0000269|PubMed:11756160, ECO:0000269|PubMed:1652061, ECO:0000269|PubMed:16950670, ECO:0000269|PubMed:17353186, ECO:0000269|PubMed:17420255, ECO:0000269|PubMed:17420256, ECO:0000269|PubMed:17972959, ECO:0000269|PubMed:18814279, ECO:0000269|PubMed:20181277, ECO:0000269|PubMed:20504948, ECO:0000269|PubMed:21610095, ECO:0000269|PubMed:21727904, ECO:0000269|PubMed:8007983, ECO:0000269|PubMed:8262059, ECO:0000269|PubMed:9312046}.

Active site (1); Beta strand (28); Binding site (1); Chain (1); Disulfide bond (4); Domain (6); Glycosylation (9); Helix (4); Modified residue (9); Mutagenesis (10); Nucleotide binding (1); Region (2); Sequence conflict (11); Signal peptide (1); Topological domain (2); Transmembrane (1); Turn (1); Widely expressed. {ECO:0000269|PubMed:20504948}.

Macrophage colony-stimulating factor 1 receptor (CSF-1 receptor) (CSF-1-R) (CSF-1R) (M-CSF-R) (EC 2.7.10.1) (Proto-oncogene c-Fms) (CD antigen CD115)

Recombinant

HEK293

Lyophilized

7.4-7.5

Sterile-filtered colorless solution

1mg/ml

Standard

PBS

SDS-PAGE

> 98%

RP-HPLC

-20°C

This bioreagent is stable at 4°C (short-term) and -70°C(long-term). After reconstitution, sample may be stored at 4°C for 2-7 days and below -18°C for future use.

Reconstitute in sterile distilled H2O to no less than 100ug/ml; dilute reconstituted stock further in other aqueous solutions if needed. Please review COA for lot-specific instructions. Final measurements should be determined by the end-user for optimal performance.