Biomarkers
Carboxypeptidase M
Recombinant ID:
3208
Gene of Interest
Gene Synonyms:
CPM
Protein Names:
Carboxypeptidase M (CPM) (EC 3.4.17.12)
Accession Data
Organism:
Homo sapiens (Human)
Mass (kDa):
50514
Length (aa):
443
Metal Binding:
METAL 83 83 Zinc; catalytic. {ECO:0000244|PDB:1UWY, ECO:0000269|PubMed:15066430}.; METAL 86 86 Zinc; catalytic. {ECO:0000244|PDB:1UWY, ECO:0000269|PubMed:15066430}.; METAL 190 190 Zinc; catalytic. {ECO:0000244|PDB:1UWY, ECO:0000269|PubMed:15066430}.
Proteomics (Proteome ID):
Carboxypeptidase M (CPM) (EC 3.4.17.12)
Proteomics (Chromosome):
UP000005640
Disease:
N/A
Mutagenesis:
MUTAGEN 277 277 E->A: 5-fold decrease in substrate affinity. 22-fold decrease in specific affinity. 104-fold decrease in catalytic efficiency. Greatly reduced heat stability. {ECO:0000269|PubMed:12457462}.; MUTAGEN 277 277 E->Q: 2-fold decrease in substrate affinity. Small increase in specific affinity. Reduced heat stability by 50%. {ECO:0000269|PubMed:12457462}.; MUTAGEN 281 281 E->Q: Abolishes enzyme activity. {ECO:0000269|PubMed:12457462}.; MUTAGEN 423 423 S->A,T: Expressed on cell membrane. Released from membrane by PI-PLC. {ECO:0000269|PubMed:12457462}.; MUTAGEN 423 423 S->P: Little expression on cell membrane. Perinuclear localization. Not released from membrane by PI-PLC. {ECO:0000269|PubMed:12457462}.
Sequence:
MDFPCLWLGLLLPLVAALDFNYHRQEGMEAFLKTVAQNYSSVTHLHSIGKSVKGRNLWVLVVGRFPKEHRIGIPEFKYVANMHGDETVGRELLLHLIDYLVTSDGKDPEITNLINSTRIHIMPSMNPDGFEAVKKPDCYYSIGRENYNQYDLNRNFPDAFEYNNVSRQPETVAVMKWLKTETFVLSANLHGGALVASYPFDNGVQATGALYSRSLTPDDDVFQYLAHTYASRNPNMKKGDECKNKMNFPNGVTNGYSWYPLQGGMQDYNYIWAQCFEITLELSCCKYPREEKLPSFWNNNKASLIEYIKQVHLGVKGQVFDQNGNPLPNVIVEVQDRKHICPYRTNKYGEYYLLLLPGSYIINVTVPGHDPHITKVIIPEKSQNFSALKKDILLPFQGQLDSIPVSNPSCPMIPLYRNLPDHSAATKPSLFLFLVSLLHIFFK
Function [CC]:
Specifically removes C-terminal basic residues (Arg or Lys) from peptides and proteins. It is believed to play important roles in the control of peptide hormone and growth factor activity at the cell surface, and in the membrane-localized degradation of extracellular proteins. {ECO:0000269|PubMed:12457462}.
Analysis Summary:
Active site (1); Beta strand (18); Chain (1); Disulfide bond (3); Glycosylation (5); Helix (13); Lipidation (1); Metal binding (3); Mutagenesis (5); Natural variant (2); Propeptide (1); Signal peptide (1); Site (1); Turn (5)
Reagent Data
Name:
Carboxypeptidase M (CPM) (EC 3.4.17.12)
Subcategory:
Recombinant
Source:
HEK293
Species:
Format:
Lyophilized
pH:
7.4-7.5
Formulation:
Sterile-filtered colorless solution
Formulation Concentration:
1mg/ml
Buffer Volume:
Standard
Buffer Solution:
PBS
Metal Chelating Agents
Determined:
SDS-PAGE
Purity:
> 98%
Validated:
RP-HPLC
Sample Handling
Storage:
-20°C
Stability:
This bioreagent is stable at 4°C (short-term) and -70°C(long-term). After reconstitution, sample may be stored at 4°C for 2-7 days and below -18°C for future use.
Preparation:
Reconstitute in sterile distilled H2O to no less than 100ug/ml; dilute reconstituted stock further in other aqueous solutions if needed. Please review COA for lot-specific instructions. Final measurements should be determined by the end-user for optimal performance.
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