GroES
Human Recombinant HSPE1
Reference ID:KB-2133
Western Blot
Gene of Interest
Gene Synonyms:HSPE1
Protein Names:10 kDa heat shock protein, mitochondrial (Hsp10) (10 kDa chaperonin) (Chaperonin 10) (CPN10) (Early-pregnancy factor) (EPF)
Accession Data
Organism:Homo sapiens (Human)
Mass (kDa):109.32
Length (aa):102
Sequence:MAGQAFRKFLPLFDRVLVERSAAETVTKGGIMLPEKSQGKVLQATVVAVGSGSKGKGGEIQPVSVKVGDKVLLPEYGGTKVVLDDKDYFLFRDGDILGKYVD
Proteomics (Proteome ID):UP000005640
Proteomics (Chromosome): Chromosome 2
Mass Spectrometry: Mass=10843.5; Mass_error=0.2; Method=Electrospray; Range=2-102; Evidence={ECO:0000269|PubMed:7912672};
Function [CC]:Co-chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp60, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix (PubMed:7912672, PubMed:1346131, PubMed:11422376). The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. In a cyclic reaction, Hsp60 ring complexes bind one unfolded substrate protein per ring, followed by the binding of ATP and association with 2 heptameric rings of the co-chaperonin Hsp10. This leads to sequestration of the substrate protein in the inner cavity of Hsp60 where, for a certain period of time, it can fold undisturbed by other cell components. Synchronous hydrolysis of ATP in all Hsp60 subunits results in the dissociation of the chaperonin rings and the release of ADP and the folded substrate protein (Probable). {ECO:0000269|PubMed:11422376, ECO:0000269|PubMed:1346131, ECO:0000269|PubMed:7912672, ECO:0000305|PubMed:25918392}.
Induction:By stress.
Reagent Data
Name:GroES
Class:Heat Shock
Subcategory:Protein
Molecular Weight:10
Source:E.Coli
Species:Human
Tag:
Amino Acid Sequence:MAGQAFRKFL PLFDRVLVER SAAETVTKGG IMLPEKSQGK VLQATVVAVG SGSKGKGGEI QPVSVKVGDK VLLPEYGGTK VVLDDKDYFL FRDGDILGKY VD
Bioactivity
Measured:1 x 10^4 Units/mg
Determined By:Assay (Variable)
Assay Profile:LOT-Specific (Check COA)
Format:Solution
Purification System:Chromatography
Formulation:Sterile-filtered colorless solution
Formulation Concentration:1 mg/ml
Buffer Volume:20 mM
Buffer Solution: Tris
pH:8
Stabilizers
NaCl:50 mM
Purity:> 98%
Determined: SDS-PAGE
Sample Handling
Storage:4°C
Stability:This bioreagent is stable at 4°C (short-term) and -70°C(long-term). After reconstitution, sample may be stored at 4°C for 2-7 days and below -18°C for future use.
Preparation:Reconstitute in sterile distilled H2O to no less than 100 ug/ml; dilute reconstituted stock further in other aqueous solutions if needed. Please review COA for lot-specific instructions. Final measurements should be determined by the end-user for optimal performance.