CCL2, biotinylated
Human Recombinant CCL2 - Biotin Tagged
Reference ID:KB-1649
Western Blot
Gene of Interest
Gene Synonyms:CCL2;MCP1;SCYA2
Protein Names:C-C motif chemokine 2 (HC11) (Monocyte chemoattractant protein 1) (Monocyte chemotactic and activating factor) (MCAF) (Monocyte chemotactic protein 1) (MCP-1) (Monocyte secretory protein JE) (Small-inducible cytokine A2)
Accession Data
Organism:Homo sapiens (Human)
Mass (kDa):110.25
Length (aa):99
Sequence:MKVSAALLCLLLIAATFIPQGLAQPDAINAPVTCCYNFTNRKISVQRLASYRRITSSKCPKEAVIFKTIVAKEICADPKQKWVQDSMDHLDKQTQTPKT
Proteomics (Proteome ID):UP000005640
Proteomics (Chromosome): Chromosome 17
Polymorphism: Genetic variations in CCL2 determine Mycobacterium tuberculosis susceptibility [MIM:607948]. {ECO:0000269|PubMed:16352737}.
Function [CC]:Acts as a ligand for C-C chemokine receptor CCR2 (PubMed:9837883, PubMed:10587439, PubMed:10529171). Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions (PubMed:9837883, PubMed:10587439). Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils (PubMed:8627182, PubMed:9792674, PubMed:8195247). May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis (PubMed:8107690). {ECO:0000269|PubMed:10529171, ECO:0000269|PubMed:10587439, ECO:0000269|PubMed:8107690, ECO:0000269|PubMed:8195247, ECO:0000269|PubMed:8627182, ECO:0000269|PubMed:9792674, ECO:0000269|PubMed:9837883}.
Site:SITE 31 31 Involved in dimerization.; SITE 36 36 Involved in dimerization, receptor binding and signaling.; SITE 41 41 Involved in GAG binding.; SITE 42 42 Involved in GAG binding.; SITE 47 47 Involved in GAG binding and receptor binding.; SITE 58 58 Involved in dimerization.; SITE 61 61 Involved in dimerization.; SITE 72 72 Involved in GAG binding and receptor binding.; SITE 81 81 Involved in GAG binding.; SITE 89 89 Involved in GAG binding.
Induction:Up-regulated upon hypertonic conditions (PubMed:23233732). In pancreatic islets, secretion is stimulated by IL1B (PubMed:23955712). {ECO:0000269|PubMed:23233732, ECO:0000269|PubMed:23955712}.
Tissue Specificity:Expressed in the seminal plasma, endometrial fluid and follicular fluid (at protein level) (PubMed:23765988). Expressed in monocytes (PubMed:2513477). {ECO:0000269|PubMed:23765988, ECO:0000269|PubMed:2513477}.
Mutagenesis:MUTAGEN 24 91 Missing: 83% reduction in activity.; MUTAGEN 24 85 Missing: 90% reduction in activity.; MUTAGEN 24 24 Missing: Loss of activity.; MUTAGEN 25 31 Missing: Loss of signaling. {ECO:0000269|PubMed:10587439}.; MUTAGEN 26 26 D->A: Reduction in activity. {ECO:0000269|PubMed:10587439}.; MUTAGEN 28 28 I->A: Slight reduction in activity. {ECO:0000269|PubMed:10587439}.; MUTAGEN 29 29 N->A: 50% reduction in activity. {ECO:0000269|PubMed:10587439}.; MUTAGEN 31 31 P->A: Loss of dimerization; slight reduction of activity. {ECO:0000269|PubMed:10587439, ECO:0000269|PubMed:9837883}.; MUTAGEN 32 32 V->A: Slight reduction in activity. {ECO:0000269|PubMed:10587439, ECO:0000269|PubMed:9837883}.; MUTAGEN 32 32 V->E: Slight reduction in affinity. {ECO:0000269|PubMed:10587439, ECO:0000269|PubMed:9837883}.; MUTAGEN 33 33 T->A: Slight reduction in activity. {ECO:0000269|PubMed:10587439, ECO:0000269|PubMed:9837883}.; MUTAGEN 33 33 T->E: Slight reduction in affinity. {ECO:0000269|PubMed:10587439, ECO:0000269|PubMed:9837883}.; MUTAGEN 36 36 Y->A: Loss of activity. {ECO:0000269|PubMed:9837883}.; MUTAGEN 47 47 R->F: 95% reduction in activity; strong reduction of receptor binding.; MUTAGEN 50 50 S->Q: 40% reduction in activity.; MUTAGEN 51 51 Y->D: Loss of activity.; MUTAGEN 53 53 R->L: Loss of activity.; MUTAGEN 79 79 K->A: No effect on heparin binding.; MUTAGEN 81 81 K->A: Strongly reduces heparin binding. {ECO:0000269|PubMed:9792674}.; MUTAGEN 89 89 H->A: Strongly reduces heparin binding. {ECO:0000269|PubMed:9792674}.; MUTAGEN 91 91 D->L: 90% reduction in activity.; MUTAGEN 95 99 Missing: No effect on heparin binding. {ECO:0000269|PubMed:9792674}.
Reagent Data
Name:CCL2, biotinylated
Class:Chemokine
Subcategory:Protein
Region:24 - 99
Molecular Weight:11.084
Source:E.Coli
Species:Human
Tag:Biotin
Amino Acid Sequence:QPDAINAPVTCCYNFTNRKISVQRLASYRRITSSKCPKEAVIFKTIVAK EICADPKQKWVQDSIDHLDKQTQTPKT
Bioactivity
Measured:Fully biologically active
Determined By:Migration Assay
Assay Profile: recombinant CCR2-expressing cells
EC50=0.25-1.2 nM
Format:Lyophilized
Buffer Volume:Standard
Buffer Solution:PBS
pH:7.4-7.5
Toxicity
Endotoxin Screened:< 0.01 EU per 1ug of protein by LAL method
Purity:> 97%
Determined: SDS-PAGE
Sample Handling
Storage:-20°C
Stability:This bioreagent is stable at 4°C (short-term) and -70°C(long-term). After reconstitution, sample may be stored at 4°C for 2-7 days and below -18°C for future use.
Preparation:Reconstitute in sterile distilled H2O to no less than 100 ug/ml; dilute reconstituted stock further in other aqueous solutions if needed. Please review COA for lot-specific instructions. Final measurements should be determined by the end-user for optimal performance.