Annexin A1
Human Recombinant ANXA1
Reference ID:KB-1428
Western Blot
Gene of Interest
Gene Synonyms:ANXA1;ANX1;LPC1
Protein Names:Annexin A1 (Annexin I) (Annexin-1) (Calpactin II) (Calpactin-2) (Chromobindin-9) (Lipocortin I) (Phospholipase A2 inhibitory protein) (p35)
Accession Data
Organism:Homo sapiens (Human)
Mass (kDa):387.14
Length (aa):346
Sequence:MAMVSEFLKQAWFIENEEQEYVQTVKSSKGGPGSAVSPYPTFNPSSDVAALHKAIMVKGVDEATIIDILTKRNNAQRQQIKAAYLQETGKPLDETLKKALTGHLEEVVLALLKTPAQFDADELRAAMKGLGTDEDTLIEILASRTNKEIRDINRVYREELKRDLAKDITSDTSGDFRNALLSLAKGDRSEDFGVNEDLADSDARALYEAGERRKGTDVNVFNTILTTRSYPQLRRVFQKYTKYSKHDMNKVLDLELKGDIEKCLTAIVKCATSKPAFFAEKLHQAMKGVGTRHKALIRIMVSRSEIDMNDIKAFYQKMYGISLCQAILDETKGDYEKILVALCGGN
Proteomics (Proteome ID):UP000005640
Proteomics (Chromosome): Chromosome 9
Function [CC]:Plays important roles in the innate immune response as effector of glucocorticoid-mediated responses and regulator of the inflammatory process. Has anti-inflammatory activity (PubMed:8425544). Plays a role in glucocorticoid-mediated down-regulation of the early phase of the inflammatory response (By similarity). Promotes resolution of inflammation and wound healing (PubMed:25664854). Functions at least in part by activating the formyl peptide receptors and downstream signaling cascades (PubMed:15187149, PubMed:25664854). Promotes chemotaxis of granulocytes and monocytes via activation of the formyl peptide receptors (PubMed:15187149). Contributes to the adaptive immune response by enhancing signaling cascades that are triggered by T-cell activation, regulates differentiation and proliferation of activated T-cells (PubMed:17008549). Promotes the differentiation of T-cells into Th1 cells and negatively regulates differentiation into Th2 cells (PubMed:17008549). Has no effect on unstimulated T cells (PubMed:17008549). Promotes rearrangement of the actin cytoskeleton, cell polarization and cell migration (PubMed:15187149). Negatively regulates hormone exocytosis via activation of the formyl peptide receptors and reorganization of the actin cytoskeleton (PubMed:19625660). Has high affinity for Ca(2+) and can bind up to eight Ca(2+) ions (By similarity). Displays Ca(2+)-dependent binding to phospholipid membranes (PubMed:2532504, PubMed:8557678). Plays a role in the formation of phagocytic cups and phagosomes. Plays a role in phagocytosis by mediating the Ca(2+)-dependent interaction between phagosomes and the actin cytoskeleton (By similarity). {ECO:0000250|UniProtKB:P10107, ECO:0000250|UniProtKB:P19619, ECO:0000269|PubMed:15187149, ECO:0000269|PubMed:17008549, ECO:0000269|PubMed:19625660, ECO:0000269|PubMed:2532504, ECO:0000269|PubMed:25664854, ECO:0000269|PubMed:2936963, ECO:0000269|PubMed:8425544, ECO:0000269|PubMed:8557678}.
Metal Binding:METAL 59 59 Calcium 1; via carbonyl oxygen. {ECO:0000250|UniProtKB:P19619}.; METAL 60 60 Calcium 1; via carbonyl oxygen. {ECO:0000250|UniProtKB:P19619}.; METAL 62 62 Calcium 1. {ECO:0000250|UniProtKB:P19619}.; METAL 97 97 Calcium 2; via carbonyl oxygen. {ECO:0000250|UniProtKB:P19619}.; METAL 100 100 Calcium 2; via carbonyl oxygen. {ECO:0000250|UniProtKB:P19619}.; METAL 105 105 Calcium 2. {ECO:0000250|UniProtKB:P19619}.; METAL 127 127 Calcium 3; via carbonyl oxygen. {ECO:0000250|UniProtKB:P19619}.; METAL 129 129 Calcium 3; via carbonyl oxygen. {ECO:0000250|UniProtKB:P19619}.; METAL 131 131 Calcium 3; via carbonyl oxygen. {ECO:0000250|UniProtKB:P19619}.; METAL 132 132 Calcium 4; via carbonyl oxygen. {ECO:0000250|UniProtKB:P19619}.; METAL 134 134 Calcium 4. {ECO:0000250|UniProtKB:P19619}.; METAL 171 171 Calcium 3. {ECO:0000250|UniProtKB:P19619}.; METAL 210 210 Calcium 5; via carbonyl oxygen. {ECO:0000250|UniProtKB:P19619}.; METAL 213 213 Calcium 5; via carbonyl oxygen. {ECO:0000250|UniProtKB:P19619}.; METAL 215 215 Calcium 5; via carbonyl oxygen. {ECO:0000250|UniProtKB:P19619}.; METAL 253 253 Calcium 6. {ECO:0000250|UniProtKB:P19619}.; METAL 255 255 Calcium 5. {ECO:0000250|UniProtKB:P19619}.; METAL 256 256 Calcium 6; via carbonyl oxygen. {ECO:0000250|UniProtKB:P19619}.; METAL 261 261 Calcium 6. {ECO:0000250|UniProtKB:P19619}.; METAL 286 286 Calcium 7; via carbonyl oxygen. {ECO:0000250|UniProtKB:P19619}.; METAL 288 288 Calcium 7; via carbonyl oxygen. {ECO:0000250|UniProtKB:P19619}.; METAL 290 290 Calcium 7; via carbonyl oxygen. {ECO:0000250|UniProtKB:P19619}.; METAL 328 328 Calcium 8; via carbonyl oxygen. {ECO:0000250|UniProtKB:P19619}.; METAL 330 330 Calcium 7. {ECO:0000250|UniProtKB:P19619}.; METAL 331 331 Calcium 8; via carbonyl oxygen. {ECO:0000250|UniProtKB:P19619}.; METAL 336 336 Calcium 8. {ECO:0000250|UniProtKB:P19619}.
Tissue Specificity:Detected in resting neutrophils (PubMed:10772777). Detected in peripheral blood T-cells (PubMed:17008549). Detected in extracellular vesicles in blood serum from patients with inflammatory bowel disease, but not in serum from healthy donors (PubMed:25664854). Detected in placenta (at protein level) (PubMed:2532504). Detected in liver. {ECO:0000269|PubMed:2532504, ECO:0000269|PubMed:2936963}.
Mutagenesis:MUTAGEN 27 27 S->A: Abolishes secretion and modulation of exocytosis. {ECO:0000269|PubMed:19625660}.; MUTAGEN 34 34 S->A: No effect on secretion and modulation of exocytosis. {ECO:0000269|PubMed:19625660}.; MUTAGEN 45 45 S->A: Abolishes secretion and nearly abolishes modulation of exocytosis. {ECO:0000269|PubMed:19625660}.
Pharmaceutical Application:Rx: Peptides based on the N-terminal sequence might be used for the treatment of inflammation, e.g. in chronic bowel diseases and in rheumatoid arthritis. {ECO:0000305}.
Miscellaneous [CC]:Was originally identified as calcium and phospholipid binding protein that displays Ca(2+)-dependent binding to phospholipid membranes and can promote membrane aggregation in vitro. Was initially identified as inhibitor of phospholipase A2 activity (in vitro) (PubMed:2936963, PubMed:8425544). Inhibition of phospholipase activity is mediated via its phospholipid binding activity that limits the access of phospholipase to its substrates. {ECO:0000269|PubMed:2936963, ECO:0000269|PubMed:8425544, ECO:0000305}.
Reagent Data
Name:Annexin A1
Class:Annexin
Subcategory:Protein
Molecular Weight:38.7
Source:E.Coli
Species:Human
Tag:
Amino Acid Sequence:MAMVSEFLKQ AWFIENEEQE YVQTVKSSKG GPGSAVSPYP TFNPSSDVAA LHKAIMVKGV DEATIIDILT KRNNAQRQQI KAAYLQETGK PLDETLKKAL TGHLEEVVLA LLKTPAQFDA DELRAAMKGL GTDEDTLIEI LASRTNKEIR DINRVYREEL KRDLAKDITS DTSGDFRNAL LSLAKGDRSE DFGVNEDLAD SDARALYEAG ERRKGTDVNV FNTILTTRSY PQLRRVFQKY TKYSKHDMNK VLDLELKGDI EKCLTAIVKCATSKPAFFAE KLHQAMKGVG TRHKALIRIM VSRSEIDMND IKAFYQKMYG ISLCQAILDE TKGDYEKILV ALCGGN
Format:Solution
Formulation:Sterile-filtered colorless solution
Formulation Concentration:1 mg/ml
Buffer Volume:Standard
Buffer Solution:PBS
pH:7.4-7.5
Purity:> 95%
Determined: SDS-PAGE
Sample Handling
Storage:4°C
Stability:This bioreagent is stable at 4°C (short-term) and -70°C(long-term). After reconstitution, sample may be stored at 4°C for 2-7 days and below -18°C for future use.
Preparation:Reconstitute in sterile distilled H2O to no less than 100 ug/ml; dilute reconstituted stock further in other aqueous solutions if needed. Please review COA for lot-specific instructions. Final measurements should be determined by the end-user for optimal performance.