Cytokines

IL-10

Recombinant ID:

3603

Gene of Interest

Gene Synonyms:

Il10;Il-10

Protein Names:

Interleukin-10 (IL-10) (Cytokine synthesis inhibitory factor) (CSIF)

Accession Data

Organism:

Mus musculus (Mouse)

Mass (kDa):

20641

Length (aa):

178

Proteomics (Proteome ID):

Interleukin-10 (IL-10) (Cytokine synthesis inhibitory factor) (CSIF)

Proteomics (Chromosome):

UP000000589

Sequence:

MPGSALLCCLLLLTGMRISRGQYSREDNNCTHFPVGQSHMLLELRTAFSQVKTFFQTKDQLDNILLTDSLMQDFKGYLGCQALSEMIQFYLVEVMPQAEKHGPEIKEHLNSLGEKLKTLRMRLRRCHRFLPCENKSKAVEQVKSDFNKLQDQGVYKAMNEFDIFINCIEAYMMIKMKS

Analysis Summary:

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (By similarity). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity) (PubMed:28473584). {ECO:0000250|UniProtKB:P22301, ECO:0000269|PubMed:28473584}.,Chain (1); Disulfide bond (2); Glycosylation (2); Helix (11); Signal peptide (1); Turn (1)

Reagent Data

Name:

Interleukin-10 (IL-10) (Cytokine synthesis inhibitory factor) (CSIF)

Subcategory:

Recombinant

Amino Acid Sequence:

Inquire

Species

Mouse

Tag:

Format:

Lyophilized

pH:

7.4-7.5

Formulation:

Sterile-filtered colorless solution

Buffer Volume

Standard

Buffer Solution:

PBS

Metal Chelating Agents

Purity:

> 98%

Determined:

SDS-PAGE

Stained:

Assay (Variable)

Validated:

RP-HPLC

Sample Handling

Storage:

-20°C

Stability:

This bioreagent is stable at 4°C (short-term) and -70°C(long-term). After reconstitution, sample may be stored at 4°C for 2-7 days and below -18°C for future use.

Preparation:

Reconstitute in sterile distilled H2O to no less than 100ug/ml; dilute reconstituted stock further in other aqueous solutions if needed. Please review COA for lot-specific instructions. Final measurements should be determined by the end-user for optimal performance.
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